LOCUS       sequence-261016-        5403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1010 encoding truncated human cytosine deaminase, hAIDx, at the 
            C-terminus. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: 1010-AIDx-CP
FEATURES             Location/Qualifiers
     source          1..5403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4183..4203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4219..4239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4861..4908
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4909..4932
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4942..4998
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggtaccgcca ccatgaatgg tgactataaa gtgtatgatg ttcgtaagat gatcgcaaag
       61 tccgagcagg agattggcaa ggcaaccgct aagtattttt tttacagcaa catcatgaac
      121 ttctttaaga ccgagattac cctggccaac ggtgaaatcc gtaaacgccc gctgattgaa
      181 accaacggtg aaaccggcga aatcgtgtgg gacaagggcc gtgattttgc gaccgtgcgc
      241 aaagtgctgt ccatgccgca ggtgaacatt gtgaagaaaa ccgaggtgca gaccggcggt
      301 ttcagcaagg aaagcattct gccgaaacgt aacagcgaca agctgatcgc ccgcaagaaa
      361 gattgggacc cgaagaaata cggcggtttc gatagcccga ccgtggctta tagcgtgctg
      421 gtggtggcca aagtggagaa gggtaaaagc aagaaactga agagcgtgaa agaactgctg
      481 ggcatcacca ttatggagcg tagcagcttc gaaaagaacc cgatcgactt tctggaggcg
      541 aagggttaca aggaagtgaa gaaagatctg atcattaagc tgccgaaata cagcctgttt
      601 gagctggaaa atggtcgtaa acgtatgctg gccagcgccg gcgagctgca aaagggcaac
      661 gaactggcgc tgccgagcaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
      721 ctgaaaggta gcccggagga caacgaacag aagcagctgt ttgtggagca gcacaaacac
      781 tatctggatg agatcattga acagatcagc gagttcagca agcgtgtgat tctggcggac
      841 gctaacctgg ataaagtgct gagcgcctac aacaaacacc gtgacaagcc gatccgcgag
      901 caggcggaaa acatcattca cctgttcacc ctgaccaacc tgggtgcgcc ggccgcgttc
      961 aagtactttg acaccaccat tgatcgtaaa cgctatacca gcaccaagga ggtgctggat
     1021 gctaccctga tccaccagag cattaccggc ctgtacgaaa cccgtattga tctgagccag
     1081 ctgggcggtg acggcggtag cggcggtagc ggcggtagcg gcggtagcgg cggtagcggc
     1141 ggtagcggcg gtgacaagaa atatagcatc ggtctggcga ttggcaccaa cagcgtgggt
     1201 tgggctgtga tcaccgatga gtacaaggtg ccgagcaaga agttcaaggt gctgggcaac
     1261 accgaccgcc acagcatcaa gaaaaacctg attggtgccc tgctgtttga tagcggtgaa
     1321 accgccgagg ccacccgtct gaagcgcacc gcccgtcgcc gttacacccg tcgtaaaaac
     1381 cgtatctgct acctgcaaga gattttcagc aacgaaatgg ccaaagtgga cgatagcttc
     1441 tttcaccgcc tggaggagag cttcctggtg gaggaagaca agaaacacga gcgtcacccg
     1501 atctttggca acattgtgga tgaggtggct taccacgaaa agtatccgac catctaccac
     1561 ctgcgtaaga aactggtgga tagcaccgac aaggccgatc tgcgcctgat ctatctggct
     1621 ctggcccaca tgatcaagtt ccgtggtcac tttctgatcg agggcgacct gaacccggat
     1681 aacagcgatg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgtttgag
     1741 gaaaacccga ttaacgccag cggtgtggac gcgaaggcta tcctgagcgc gcgtctgagc
     1801 aaaagccgcc gtctggagaa cctgatcgcg cagctgccgg gtgaaaagaa aaacggtctg
     1861 tttggcaacc tgattgctct gagcctgggc ctgaccccga acttcaaaag caactttgac
     1921 ctggcggagg atgctaagct gcaactgagc aaggatacct atgacgatga cctggacaac
     1981 ctgctggcgc agatcggcga ccagtacgct gatctgttcc tggctgccaa gaacctgagc
     2041 gatgcgatcc tgctgagcga cattctgcgt gtgaacaccg agattaccaa ggccccgctg
     2101 agcgcgagca tgatcaaacg ctatgatgaa caccaccagg acctgaccct gctgaaagct
     2161 ctggtgcgtc agcagctgcc ggagaagtac aaagaaatct tctttgacca gagcaagaac
     2221 ggttacgccg gctatattga tggcggtgcg agccaggagg agttctacaa gtttatcaaa
     2281 ccgattctgg agaaaatgga cggcaccgag gaactgctgg tgaagctgaa ccgtgaagat
     2341 ctgctgcgta aacagcgcac cttcgacaac ggtagcatcc cgcaccagat tcacctgggt
     2401 gagctgcacg ccatcctgcg ccgtcaggaa gatttctacc cgtttctgaa ggacaaccgt
     2461 gagaagatcg aaaaaattct gaccttccgc attccgtact atgtgggtcc gctggctcgt
     2521 ggcaacagcc gctttgcctg gatgacccgc aagagcgagg aaaccatcac cccgtggaac
     2581 ttcgaggaag tggtggacaa aggcgccagc gcgcagagct tcattgagcg tatgaccaac
     2641 tttgataaga acctgccgaa cgaaaaagtg ctgccgaagc acagcctgct gtacgagtat
     2701 ttcaccgtgt acaacgaact gaccaaggtg aaatatgtga ccgagggtat gcgcaagccg
     2761 gccttcctga gcggcgaaca gaagaaagcg atcgtggacc tgctgtttaa aaccaaccgt
     2821 aaggtgaccg tgaagcagct gaaagaggat tatttcaaga aaattgaatg ctttgacagc
     2881 gtggagatca gcggtgtgga agatcgtttt aacgcgagcc tgggcaccta ccacgacctg
     2941 ctgaaaatca ttaaggataa agacttcctg gataacgagg aaaacgagga catcctggaa
     3001 gatattgtgc tgaccctgac cctgtttgag gaccgtgaaa tgatcgagga acgcctgaag
     3061 acctatgccc acctgttcga tgacaaagtg atgaaacagc tgaagcgccg tcgctacacc
     3121 ggttggggcc gtctgagccg caaactgatc aacggtattc gcgataaaca gagcggcaag
     3181 accatcctgg attttctgaa gagcgacggt ttcgcgaacc gtaactttat gcagctgatt
     3241 cacgatgaca gcctgacctt caaagaggac atccagaagg cgcaggtgag cggtcagggc
     3301 gatagcctgc acgaacatat tgctaacctg gccggtagcc cggccatcaa gaagggcatc
     3361 ctgcaaaccg tgaaggtggt ggacgagctg gtgaaagtga tgggtcgtca caagccggaa
     3421 aacatcgtga ttgagatggc ccgcgaaaac cagaccaccc agaaaggcca gaagaacagc
     3481 cgtgagcgca tgaagcgtat cgaggaaggc atcaaggaac tgggcagcca gatcctgaag
     3541 gagcacccgg tggaaaacac ccagctgcaa aacgagaaac tgtacctgta ctacctgcaa
     3601 aacggtcgtg acatgtatgt ggatcaggaa ctggacatta accgcctgag cgattatgac
     3661 gtggatgcga tcgtgccgca gagcttcctg aaggatgaca gcattgacaa caaagtgctg
     3721 acccgtagcg ataaaaaccg cggcaagagc gacaacgtgc cgagcgagga agtggtgaag
     3781 aaaatgaaaa actactggcg tcagctgctg aacgccaaac tgatcaccca gcgtaagttt
     3841 gacaacctga ccaaagctga gcgcggcggt ctgagcgaac tggataaagc cggtttcatt
     3901 aagcgtcagc tggtggaaac ccgccagatc accaagcacg tggcgcagat tctggacagc
     3961 cgcatgaaca ccaagtatga cgagaacgat aaactgatcc gtgaagtgaa agtgatcacc
     4021 ctgaagagca aactggtgag cgattttcgt aaagacttcc agttttacaa ggtgcgcgag
     4081 atcaacaact accaccacgc tcacgacgcc tatctgaacg cggtggtggg caccgccctg
     4141 atcaagaagt acccgaagct ggagagcgag ttcgtgtacg gcccgaagaa aaagcgtaaa
     4201 gtgggtagca gcggcagccc gaaaaagaaa cgcaaagtgg gtagcgacgc tctggatgac
     4261 ttcgacctgg atatgctggg cagcgacgcc ctggatgact ttggcggtgg cagcatggat
     4321 agcctgctga tgaaccgtcg caaattcctg tatcagttta agaacgtgcg ttgggctaaa
     4381 ggccgtcgcg aaacctacct gtgctatgtg gtgaaacgtc gcgatagcgc caccagcttc
     4441 agcctggact ttggttacct gcgcaacaag aacggctgcc acgtggagct gctgttcctg
     4501 cgttacatta gcgactggga cctggaccca ggccgttgct atcgcgtgac ctggtttacc
     4561 agctggagcc cgtgctacga ttgcgctcgt catgtggcgg acttcctgcg tggtaaccca
     4621 aacctgagcc tgcgtatctt caccgcgcgc ctgtactttt gcgaggaccg taaggctgag
     4681 ccggaaggtc tgcgtcgcct gcaccgtgcg ggtgtgcaga tcgcgatcat gaccttcaaa
     4741 gattactttt attgctggaa caccttcgtg gagaaccacg aacgcacctt taaggcctgg
     4801 gagggtctgc acgaaaacag cgtgcgtctg agccgccagc tgcgccgcat cctgctgcca
     4861 aagcgtccgg cggctaccaa gaaagcgggc caggctaaga aaaagaaaga ctacaaagat
     4921 gacgatgaca agggtagcgg cgctaccaac ttcagcctgc tgaagcaggc gggcgacgtg
     4981 gaggaaaatc cgggcccgat ggctaaaccg ctgagccagg aggaaagcac cctgatcgag
     5041 cgcgctaccg ccaccattaa cagcatcccg attagcgaag actatagcgt ggccagcgcc
     5101 gcgctgagca gcgatggtcg tattttcacc ggcgtgaacg tgtaccactt taccggtggc
     5161 ccgtgcgccg agctggtggt gctgggcacc gccgccgccg ccgccgccgg caacctgacc
     5221 tgcatcgtgg cgattggtaa cgaaaatcgt ggcatcctga gcccgtgcgg ccgttgccgc
     5281 caggtgctgc tggacctgca tccgggcatc aaggcgattg tgaaggatag cgacggccaa
     5341 ccgaccgccg tgggtattcg tgaactgctg ccatccggct atgtttggga gggctaagga
     5401 tcc
//
