LOCUS       sequence-261018-        5400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1058 encoding truncated human cytosine deaminase, hAIDx, at the 
            C-terminus. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: 1058-AIDx-CP
FEATURES             Location/Qualifiers
     source          1..5400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4180..4200
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4216..4236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4858..4905
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4906..4929
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4939..4995
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggtaccgcca ccatgaatcg taaacgcccg ctgattgaaa ccaacggtga aaccggcgaa
       61 atcgtgtggg acaagggccg tgattttgcg accgtgcgca aagtgctgtc catgccgcag
      121 gtgaacattg tgaagaaaac cgaggtgcag accggcggtt tcagcaagga aagcattctg
      181 ccgaaacgta acagcgacaa gctgatcgcc cgcaagaaag attgggaccc gaagaaatac
      241 ggcggtttcg atagcccgac cgtggcttat agcgtgctgg tggtggccaa agtggagaag
      301 ggtaaaagca agaaactgaa gagcgtgaaa gaactgctgg gcatcaccat tatggagcgt
      361 agcagcttcg aaaagaaccc gatcgacttt ctggaggcga agggttacaa ggaagtgaag
      421 aaagatctga tcattaagct gccgaaatac agcctgtttg agctggaaaa tggtcgtaaa
      481 cgtatgctgg ccagcgccgg cgagctgcaa aagggcaacg aactggcgct gccgagcaaa
      541 tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaaaggtag cccggaggac
      601 aacgaacaga agcagctgtt tgtggagcag cacaaacact atctggatga gatcattgaa
      661 cagatcagcg agttcagcaa gcgtgtgatt ctggcggacg ctaacctgga taaagtgctg
      721 agcgcctaca acaaacaccg tgacaagccg atccgcgagc aggcggaaaa catcattcac
      781 ctgttcaccc tgaccaacct gggtgcgccg gccgcgttca agtactttga caccaccatt
      841 gatcgtaaac gctataccag caccaaggag gtgctggatg ctaccctgat ccaccagagc
      901 attaccggcc tgtacgaaac ccgtattgat ctgagccagc tgggcggtga cggcggtagc
      961 ggcggtagcg gcggtagcgg cggtagcggc ggtagcggcg gtagcggcgg tgacaagaaa
     1021 tatagcatcg gtctggcgat tggcaccaac agcgtgggtt gggctgtgat caccgatgag
     1081 tacaaggtgc cgagcaagaa gttcaaggtg ctgggcaaca ccgaccgcca cagcatcaag
     1141 aaaaacctga ttggtgccct gctgtttgat agcggtgaaa ccgccgaggc cacccgtctg
     1201 aagcgcaccg cccgtcgccg ttacacccgt cgtaaaaacc gtatctgcta cctgcaagag
     1261 attttcagca acgaaatggc caaagtggac gatagcttct ttcaccgcct ggaggagagc
     1321 ttcctggtgg aggaagacaa gaaacacgag cgtcacccga tctttggcaa cattgtggat
     1381 gaggtggctt accacgaaaa gtatccgacc atctaccacc tgcgtaagaa actggtggat
     1441 agcaccgaca aggccgatct gcgcctgatc tatctggctc tggcccacat gatcaagttc
     1501 cgtggtcact ttctgatcga gggcgacctg aacccggata acagcgatgt ggacaagctg
     1561 ttcatccagc tggtgcagac ctacaaccag ctgtttgagg aaaacccgat taacgccagc
     1621 ggtgtggacg cgaaggctat cctgagcgcg cgtctgagca aaagccgccg tctggagaac
     1681 ctgatcgcgc agctgccggg tgaaaagaaa aacggtctgt ttggcaacct gattgctctg
     1741 agcctgggcc tgaccccgaa cttcaaaagc aactttgacc tggcggagga tgctaagctg
     1801 caactgagca aggataccta tgacgatgac ctggacaacc tgctggcgca gatcggcgac
     1861 cagtacgctg atctgttcct ggctgccaag aacctgagcg atgcgatcct gctgagcgac
     1921 attctgcgtg tgaacaccga gattaccaag gccccgctga gcgcgagcat gatcaaacgc
     1981 tatgatgaac accaccagga cctgaccctg ctgaaagctc tggtgcgtca gcagctgccg
     2041 gagaagtaca aagaaatctt ctttgaccag agcaagaacg gttacgccgg ctatattgat
     2101 ggcggtgcga gccaggagga gttctacaag tttatcaaac cgattctgga gaaaatggac
     2161 ggcaccgagg aactgctggt gaagctgaac cgtgaagatc tgctgcgtaa acagcgcacc
     2221 ttcgacaacg gtagcatccc gcaccagatt cacctgggtg agctgcacgc catcctgcgc
     2281 cgtcaggaag atttctaccc gtttctgaag gacaaccgtg agaagatcga aaaaattctg
     2341 accttccgca ttccgtacta tgtgggtccg ctggctcgtg gcaacagccg ctttgcctgg
     2401 atgacccgca agagcgagga aaccatcacc ccgtggaact tcgaggaagt ggtggacaaa
     2461 ggcgccagcg cgcagagctt cattgagcgt atgaccaact ttgataagaa cctgccgaac
     2521 gaaaaagtgc tgccgaagca cagcctgctg tacgagtatt tcaccgtgta caacgaactg
     2581 accaaggtga aatatgtgac cgagggtatg cgcaagccgg ccttcctgag cggcgaacag
     2641 aagaaagcga tcgtggacct gctgtttaaa accaaccgta aggtgaccgt gaagcagctg
     2701 aaagaggatt atttcaagaa aattgaatgc tttgacagcg tggagatcag cggtgtggaa
     2761 gatcgtttta acgcgagcct gggcacctac cacgacctgc tgaaaatcat taaggataaa
     2821 gacttcctgg ataacgagga aaacgaggac atcctggaag atattgtgct gaccctgacc
     2881 ctgtttgagg accgtgaaat gatcgaggaa cgcctgaaga cctatgccca cctgttcgat
     2941 gacaaagtga tgaaacagct gaagcgccgt cgctacaccg gttggggccg tctgagccgc
     3001 aaactgatca acggtattcg cgataaacag agcggcaaga ccatcctgga ttttctgaag
     3061 agcgacggtt tcgcgaaccg taactttatg cagctgattc acgatgacag cctgaccttc
     3121 aaagaggaca tccagaaggc gcaggtgagc ggtcagggcg atagcctgca cgaacatatt
     3181 gctaacctgg ccggtagccc ggccatcaag aagggcatcc tgcaaaccgt gaaggtggtg
     3241 gacgagctgg tgaaagtgat gggtcgtcac aagccggaaa acatcgtgat tgagatggcc
     3301 cgcgaaaacc agaccaccca gaaaggccag aagaacagcc gtgagcgcat gaagcgtatc
     3361 gaggaaggca tcaaggaact gggcagccag atcctgaagg agcacccggt ggaaaacacc
     3421 cagctgcaaa acgagaaact gtacctgtac tacctgcaaa acggtcgtga catgtatgtg
     3481 gatcaggaac tggacattaa ccgcctgagc gattatgacg tggatgcgat cgtgccgcag
     3541 agcttcctga aggatgacag cattgacaac aaagtgctga cccgtagcga taaaaaccgc
     3601 ggcaagagcg acaacgtgcc gagcgaggaa gtggtgaaga aaatgaaaaa ctactggcgt
     3661 cagctgctga acgccaaact gatcacccag cgtaagtttg acaacctgac caaagctgag
     3721 cgcggcggtc tgagcgaact ggataaagcc ggtttcatta agcgtcagct ggtggaaacc
     3781 cgccagatca ccaagcacgt ggcgcagatt ctggacagcc gcatgaacac caagtatgac
     3841 gagaacgata aactgatccg tgaagtgaaa gtgatcaccc tgaagagcaa actggtgagc
     3901 gattttcgta aagacttcca gttttacaag gtgcgcgaga tcaacaacta ccaccacgct
     3961 cacgacgcct atctgaacgc ggtggtgggc accgccctga tcaagaagta cccgaagctg
     4021 gagagcgagt tcgtgtacgg cgactataaa gtgtatgatg ttcgtaagat gatcgcaaag
     4081 tccgagcagg agattggcaa ggcaaccgct aagtattttt tttacagcaa catcatgaac
     4141 ttctttaaga ccgagattac cctggccaac ggtgaaatcc cgaagaaaaa gcgtaaagtg
     4201 ggtagcagcg gcagcccgaa aaagaaacgc aaagtgggta gcgacgctct ggatgacttc
     4261 gacctggata tgctgggcag cgacgccctg gatgactttg gcggtggcag catggatagc
     4321 ctgctgatga accgtcgcaa attcctgtat cagtttaaga acgtgcgttg ggctaaaggc
     4381 cgtcgcgaaa cctacctgtg ctatgtggtg aaacgtcgcg atagcgccac cagcttcagc
     4441 ctggactttg gttacctgcg caacaagaac ggctgccacg tggagctgct gttcctgcgt
     4501 tacattagcg actgggacct ggacccaggc cgttgctatc gcgtgacctg gtttaccagc
     4561 tggagcccgt gctacgattg cgctcgtcat gtggcggact tcctgcgtgg taacccaaac
     4621 ctgagcctgc gtatcttcac cgcgcgcctg tacttttgcg aggaccgtaa ggctgagccg
     4681 gaaggtctgc gtcgcctgca ccgtgcgggt gtgcagatcg cgatcatgac cttcaaagat
     4741 tacttttatt gctggaacac cttcgtggag aaccacgaac gcacctttaa ggcctgggag
     4801 ggtctgcacg aaaacagcgt gcgtctgagc cgccagctgc gccgcatcct gctgccaaag
     4861 cgtccggcgg ctaccaagaa agcgggccag gctaagaaaa agaaagacta caaagatgac
     4921 gatgacaagg gtagcggcgc taccaacttc agcctgctga agcaggcggg cgacgtggag
     4981 gaaaatccgg gcccgatggc taaaccgctg agccaggagg aaagcaccct gatcgagcgc
     5041 gctaccgcca ccattaacag catcccgatt agcgaagact atagcgtggc cagcgccgcg
     5101 ctgagcagcg atggtcgtat tttcaccggc gtgaacgtgt accactttac cggtggcccg
     5161 tgcgccgagc tggtggtgct gggcaccgcc gccgccgccg ccgccggcaa cctgacctgc
     5221 atcgtggcga ttggtaacga aaatcgtggc atcctgagcc cgtgcggccg ttgccgccag
     5281 gtgctgctgg acctgcatcc gggcatcaag gcgattgtga aggatagcga cggccaaccg
     5341 accgccgtgg gtattcgtga actgctgcca tccggctatg tttgggaggg ctaaggatcc
//
