LOCUS       sequence-261020-        5400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of intradomain insertion of truncated human cytosine 
            deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: AIDx-ID-202
FEATURES             Location/Qualifiers
     source          1..5400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             619..639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             655..675
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4858..4905
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4906..4929
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4939..4995
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aagcttgcca ccatggataa gaaatatagc atcggcctgg caatcggcac caatagcgtg
       61 ggctgggctg tgattaccga cgaatacaaa gttccgtcca agaagttcaa ggtgctgggt
      121 aacaccgacc gccacagcat caagaaaaac ctgattggtg ccctgctgtt tgatagcggt
      181 gaaaccgccg aggctacccg tctgaagcgc accgcccgtc gccgttatac ccgtcgtaaa
      241 aaccgtatct gctacctgca agagattttc agcaacgaaa tggccaaagt ggacgatagc
      301 ttctttcacc gcctggagga gagcttcctg gtggaggaag acaagaaaca cgagcgtcac
      361 ccgatctttg gcaacattgt ggatgaggtg gcttatcacg aaaagtaccc gaccatctat
      421 cacctgcgta agaaactggt ggatagcacc gacaaggccg atctgcgcct gatctacctg
      481 gcgctggctc acatgatcaa gttccgtggt cactttctga tcgagggcga cctgaacccg
      541 gataacagcg atgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttt
      601 gaggaaaacc cgattaaccc gaagaaaaag cgtaaagtgg gtagcagcgg cagcccgaaa
      661 aagaaacgca aagtgggtag cgacgccctg gacgatttcg atctggacat gctgggcagc
      721 gacgcgctgg acgattttgg cggtggcagc atggatagcc tgctgatgaa ccgccgtaaa
      781 ttcctgtacc agtttaagaa cgtgcgttgg gctaaaggtc gccgtgaaac ctacctgtgc
      841 tatgtggtga aacgccgtga tagcgccacc agcttcagcc tggactttgg ttatctgcgc
      901 aacaagaacg gctgccacgt ggagctgctg ttcctgcgtt atatcagcga ttgggacctg
      961 gacccaggcc gttgctaccg tgtgacctgg ttcaccagct ggagcccgtg ctatgattgc
     1021 gctcgtcacg tggccgactt tctgcgcggc aacccgaacc tgagcctgcg tatcttcacc
     1081 gcgcgcctgt acttttgcga ggaccgtaag gctgagccgg aaggtctgcg ccgtctgcac
     1141 cgtgcgggtg tgcagatcgc gatcatgacc ttcaaagatt acttttattg ctggaacacc
     1201 ttcgtggaga accacgaacg tacctttaag gcgtgggagg gtctgcacga aaacagcgtg
     1261 cgtctgagcc gccagctgcg ccgtatcctg ctgccgggtg gcagcggtgg cagcggtggc
     1321 agcggtggca gcggtggcag cggtggcagc ggtggcaatg ccagcggtgt ggacgctaag
     1381 gccatcctga gcgcgcgtct gagcaaaagc cgccgtctgg agaacctgat tgcgcagctg
     1441 ccgggtgaaa agaaaaacgg tctgtttggc aacctgattg ctctgagcct gggcctgacc
     1501 ccgaacttca aaagcaactt tgacctggcc gaggatgcga agctgcaact gagcaaggat
     1561 acctacgacg atgacctgga caacctgctg gcgcagatcg gcgaccagta tgctgatctg
     1621 ttcctggccg cgaagaacct gagcgatgcg atcctgctga gcgacattct gcgtgtgaac
     1681 accgagatta ccaaggcgcc gctgagcgcc agcatgatca aacgctacga tgaacaccac
     1741 caggacctga ccctgctgaa agctctggtg cgtcagcagc tgccggagaa gtataaagaa
     1801 atcttctttg accagagcaa gaacggttac gccggctata ttgatggtgg cgcgagccag
     1861 gaggagttct acaagtttat caaaccgatt ctggagaaga tggacggcac cgaggaactg
     1921 ctggtgaagc tgaaccgtga agatctgctg cgtaaacagc gcaccttcga caacggtagc
     1981 atcccgcacc agattcacct gggtgagctg cacgccatcc tgcgccgtca ggaagatttc
     2041 tacccgtttc tgaaggacaa ccgtgagaag atcgaaaaaa ttctgacctt ccgcattccg
     2101 tactatgtgg gtccgctggc tcgtggcaac agccgctttg cctggatgac ccgcaagagc
     2161 gaggaaacca tcaccccgtg gaacttcgag gaagtggtgg acaaaggcgc cagcgcccag
     2221 agcttcattg agcgtatgac caactttgat aagaacctgc cgaacgaaaa agtgctgccg
     2281 aagcacagcc tgctgtacga gtatttcacc gtgtataacg aactgaccaa ggtgaaatac
     2341 gtgaccgagg gtatgcgcaa gccggccttc ctgagcggcg aacagaagaa agcgatcgtg
     2401 gacctgctgt ttaaaaccaa ccgtaaggtg accgtgaagc agctgaaaga ggattacttc
     2461 aagaaaattg aatgcttcga cagcgtggag atcagcggtg tggaagatcg ttttaacgcg
     2521 agcctgggca cctatcacga cctgctgaaa atcattaagg acaaagattt cctggataac
     2581 gaggaaaacg aggacatcct ggaagatatt gtgctgaccc tgaccctgtt tgaggaccgt
     2641 gaaatgatcg aggaacgcct gaagacctac gcccacctgt tcgatgacaa agtgatgaaa
     2701 cagctgaagc gccgtcgcta taccggttgg ggccgtctga gccgcaaact gatcaacggt
     2761 attcgcgata aacagagcgg caagaccatc ctggattttc tgaagagcga cggtttcgcg
     2821 aaccgtaact ttatgcagct gattcacgat gacagcctga ccttcaaaga ggacatccag
     2881 aaggcgcagg tgagcggtca gggcgatagc ctgcacgaac acatcgctaa cctggccggt
     2941 agcccggcca tcaagaaggg catcctgcaa accgtgaagg tggtggacga gctggtgaaa
     3001 gtgatgggtc gtcacaaacc ggaaaacatc gtgattgaga tggcccgcga aaaccagacc
     3061 acccagaaag gccagaagaa cagccgtgag cgcatgaagc gtatcgagga aggcatcaag
     3121 gaactgggca gccagatcct gaaggagcac ccggtggaaa acacccagct gcaaaacgag
     3181 aaactgtatc tgtactacct gcaaaacggt cgtgacatgt acgtggatca ggagctggac
     3241 attaaccgcc tgagcgacta cgatgtggac gcgatcgtgc cgcagagctt cctgaaggat
     3301 gacagcattg acaacaaagt gctgacccgt agcgataaaa accgcggcaa gagcgacaac
     3361 gtgccgagcg aggaagtggt gaagaaaatg aaaaactact ggcgtcagct gctgaacgcc
     3421 aagctgatca cccagcgtaa atttgacaac ctgaccaagg ctgagcgcgg tggcctgagc
     3481 gaactggata aagccggttt cattaagcgt cagctggtgg aaacccgcca gatcaccaag
     3541 cacgtggcgc agattctgga cagccgcatg aacaccaaat acgacgagaa cgataagctg
     3601 atccgtgaag tgaaagtgat taccctgaag agcaaactgg tgagcgattt tcgtaaggac
     3661 ttccagtttt acaaagtgcg cgagatcaac aactatcacc acgcgcacga cgcttacctg
     3721 aacgctgtgg tgggcaccgc cctgatcaag aagtatccga agctggagag cgagttcgtg
     3781 tacggcgatt ataaagtgta cgacgtgcgt aaaatgatcg ccaagagcga gcaggaaatt
     3841 ggcaaagcga ccgctaagta tttcttttac agcaacatca tgaacttctt taagaccgag
     3901 atcaccctgg ccaacggtga aatccgtaaa cgcccgctga ttgaaaccaa cggtgaaacc
     3961 ggcgaaatcg tgtgggataa gggccgtgac tttgcgaccg tgcgcaaagt gctgtccatg
     4021 ccgcaggtga acattgtgaa gaaaaccgag gtgcagaccg gtggcttcag caaggaaagc
     4081 attctgccga aacgtaacag cgacaagctg atcgcccgca agaaagattg ggacccgaag
     4141 aaatatggtg gcttcgatag cccaaccgtg gcctacagcg tgctggtggt ggctaaagtg
     4201 gagaagggta aaagcaagaa actgaaaagc gtgaaggaac tgctgggcat caccattatg
     4261 gagcgtagca gcttcgaaaa gaacccgatc gactttctgg aggcgaaagg ttacaaggaa
     4321 gtgaagaaag atctgatcat caagctgccg aagtatagcc tgtttgagct ggaaaatggt
     4381 cgtaaacgta tgctggccag cgccggcgag ctgcaaaagg gcaacgaact ggcgctgccg
     4441 agcaaatacg tgaacttcct gtatctggcc agccactacg agaagctgaa aggtagcccg
     4501 gaggacaacg aacagaagca gctgtttgtg gagcagcaca aacactacct ggatgagatc
     4561 attgaacaga ttagcgagtt cagcaagcgt gtgatcctgg ctgatgccaa cctggacaaa
     4621 gtgctgagcg cctataacaa acaccgtgac aagccgatcc gcgagcaggc cgaaaacatc
     4681 attcacctgt ttaccctgac caacctgggc gcgccggctg ccttcaaata ttttgatacc
     4741 accattgacc gtaagcgtta caccagcacc aaagaggtgc tggatgctac cctgatccac
     4801 cagagcatta ccggtctgta cgaaacccgt atcgatctga gccagctggg tggcgacaag
     4861 cgcccggcgg ctaccaagaa agccggtcag gcgaagaaaa agaaagacta caaggatgac
     4921 gatgacaagg gtagcggcgc taccaacttc agcctgctga agcaggcggg cgacgtggag
     4981 gaaaatccgg gcccgatggc taaaccgctg agccaggagg aaagcaccct gatcgagcgc
     5041 gcgaccgcta ccattaacag catcccgatt agcgaagatt atagcgtggc cagcgccgcg
     5101 ctgagcagcg atggtcgtat cttcaccggc gtgaacgtgt atcactttac cggtggcccg
     5161 tgcgccgagc tggtggtgct gggcaccgcc gccgccgccg ccgccggcaa cctgacctgc
     5221 atcgtggcga ttggtaacga aaatcgtggc atcctgagcc cgtgcggccg ttgccgccag
     5281 gtgctgctgg acctgcatcc gggcatcaag gcgattgtga aggatagcga cggccaaccg
     5341 accgccgtgg gtattcgtga actgctgcca tccggctatg tttgggaagg ctaaggtacc
//
