LOCUS       sequence-261030-        5673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of nSpyCas9 encoding truncated human cytosine deaminase 
            hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) 
            at the C terminus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 AIDx with UGI
FEATURES             Location/Qualifiers
     source          1..5673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..264
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4453..4473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4489..4509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5131..5178
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5179..5202
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5212..5268
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggtaccgcca ccatgaccaa cctgagcgac atcattgaga aagagaccgg caagcagctg
       61 gtaatccagg aaagcattct gatgctgccg gaagaagtgg aggaagtgat tggtaacaaa
      121 ccggaaagcg acatcctggt gcacaccgct tatgacgaga gcaccgacga aaacgtgatg
      181 ctgctgacca gcgacgcgcc ggagtataag ccgtgggcgc tggtaattca ggatagcaac
      241 ggcgaaaaca aaatcaagat gctgggcggc gatagcggtg gtagcatagg caaggcaacc
      301 gctaagtatt ttttttacag caacatcatg aacttcttta agaccgagat taccctggcc
      361 aacggtgaaa tccgtaaacg cccgctgatt gaaaccaacg gtgaaaccgg cgaaatcgtg
      421 tgggacaagg gccgtgattt tgcgaccgtg cgcaaagtgc tgtccatgcc gcaggtgaac
      481 attgtgaaga aaaccgaggt gcagaccggc ggtttcagca aggaaagcat tctgccgaaa
      541 cgtaacagcg acaagctgat cgcccgcaag aaagattggg acccgaagaa atacggcggt
      601 ttcgatagcc cgaccgtggc ttatagcgtg ctggtggtgg ccaaagtgga gaagggtaaa
      661 agcaagaaac tgaagagcgt gaaagaactg ctgggcatca ccattatgga gcgtagcagc
      721 ttcgaaaaga acccgatcga ctttctggag gcgaagggtt acaaggaagt gaagaaagat
      781 ctgatcatta agctgccgaa atacagcctg tttgagctgg aaaatggtcg taaacgtatg
      841 ctggccagcg ccggcgagct gcaaaagggc aacgaactgg cgctgccgag caaatatgtg
      901 aacttcctgt acctggccag ccactatgag aagctgaaag gtagcccgga ggacaacgaa
      961 cagaagcagc tgtttgtgga gcagcacaaa cactatctgg atgagatcat tgaacagatc
     1021 agcgagttca gcaagcgtgt gattctggcg gacgctaacc tggataaagt gctgagcgcc
     1081 tacaacaaac accgtgacaa gccgatccgc gagcaggcgg aaaacatcat tcacctgttc
     1141 accctgacca acctgggtgc gccggccgcg ttcaagtact ttgacaccac cattgatcgt
     1201 aaacgctata ccagcaccaa ggaggtgctg gatgctaccc tgatccacca gagcattacc
     1261 ggcctgtacg aaacccgtat tgatctgagc cagctgggcg gtgacggcgg tagcggcggt
     1321 agcggcggta gcggcggtag cggcggtagc ggcggtagcg gcggtgacaa gaaatatagc
     1381 atcggtctgg cgattggcac caacagcgtg ggttgggctg tgatcaccga tgagtacaag
     1441 gtgccgagca agaagttcaa ggtgctgggc aacaccgacc gccacagcat caagaaaaac
     1501 ctgattggtg ccctgctgtt tgatagcggt gaaaccgccg aggccacccg tctgaagcgc
     1561 accgcccgtc gccgttacac ccgtcgtaaa aaccgtatct gctacctgca agagattttc
     1621 agcaacgaaa tggccaaagt ggacgatagc ttctttcacc gcctggagga gagcttcctg
     1681 gtggaggaag acaagaaaca cgagcgtcac ccgatctttg gcaacattgt ggatgaggtg
     1741 gcttaccacg aaaagtatcc gaccatctac cacctgcgta agaaactggt ggatagcacc
     1801 gacaaggccg atctgcgcct gatctatctg gctctggccc acatgatcaa gttccgtggt
     1861 cactttctga tcgagggcga cctgaacccg gataacagcg atgtggacaa gctgttcatc
     1921 cagctggtgc agacctacaa ccagctgttt gaggaaaacc cgattaacgc cagcggtgtg
     1981 gacgcgaagg ctatcctgag cgcgcgtctg agcaaaagcc gccgtctgga gaacctgatc
     2041 gcgcagctgc cgggtgaaaa gaaaaacggt ctgtttggca acctgattgc tctgagcctg
     2101 ggcctgaccc cgaacttcaa aagcaacttt gacctggcgg aggatgctaa gctgcaactg
     2161 agcaaggata cctatgacga tgacctggac aacctgctgg cgcagatcgg cgaccagtac
     2221 gctgatctgt tcctggctgc caagaacctg agcgatgcga tcctgctgag cgacattctg
     2281 cgtgtgaaca ccgagattac caaggccccg ctgagcgcga gcatgatcaa acgctatgat
     2341 gaacaccacc aggacctgac cctgctgaaa gctctggtgc gtcagcagct gccggagaag
     2401 tacaaagaaa tcttctttga ccagagcaag aacggttacg ccggctatat tgatggcggt
     2461 gcgagccagg aggagttcta caagtttatc aaaccgattc tggagaaaat ggacggcacc
     2521 gaggaactgc tggtgaagct gaaccgtgaa gatctgctgc gtaaacagcg caccttcgac
     2581 aacggtagca tcccgcacca gattcacctg ggtgagctgc acgccatcct gcgccgtcag
     2641 gaagatttct acccgtttct gaaggacaac cgtgagaaga tcgaaaaaat tctgaccttc
     2701 cgcattccgt actatgtggg tccgctggct cgtggcaaca gccgctttgc ctggatgacc
     2761 cgcaagagcg aggaaaccat caccccgtgg aacttcgagg aagtggtgga caaaggcgcc
     2821 agcgcgcaga gcttcattga gcgtatgacc aactttgata agaacctgcc gaacgaaaaa
     2881 gtgctgccga agcacagcct gctgtacgag tatttcaccg tgtacaacga actgaccaag
     2941 gtgaaatatg tgaccgaggg tatgcgcaag ccggccttcc tgagcggcga acagaagaaa
     3001 gcgatcgtgg acctgctgtt taaaaccaac cgtaaggtga ccgtgaagca gctgaaagag
     3061 gattatttca agaaaattga atgctttgac agcgtggaga tcagcggtgt ggaagatcgt
     3121 tttaacgcga gcctgggcac ctaccacgac ctgctgaaaa tcattaagga taaagacttc
     3181 ctggataacg aggaaaacga ggacatcctg gaagatattg tgctgaccct gaccctgttt
     3241 gaggaccgtg aaatgatcga ggaacgcctg aagacctatg cccacctgtt cgatgacaaa
     3301 gtgatgaaac agctgaagcg ccgtcgctac accggttggg gccgtctgag ccgcaaactg
     3361 atcaacggta ttcgcgataa acagagcggc aagaccatcc tggattttct gaagagcgac
     3421 ggtttcgcga accgtaactt tatgcagctg attcacgatg acagcctgac cttcaaagag
     3481 gacatccaga aggcgcaggt gagcggtcag ggcgatagcc tgcacgaaca tattgctaac
     3541 ctggccggta gcccggccat caagaagggc atcctgcaaa ccgtgaaggt ggtggacgag
     3601 ctggtgaaag tgatgggtcg tcacaagccg gaaaacatcg tgattgagat ggcccgcgaa
     3661 aaccagacca cccagaaagg ccagaagaac agccgtgagc gcatgaagcg tatcgaggaa
     3721 ggcatcaagg aactgggcag ccagatcctg aaggagcacc cggtggaaaa cacccagctg
     3781 caaaacgaga aactgtacct gtactacctg caaaacggtc gtgacatgta tgtggatcag
     3841 gaactggaca ttaaccgcct gagcgattat gacgtggatg cgatcgtgcc gcagagcttc
     3901 ctgaaggatg acagcattga caacaaagtg ctgacccgta gcgataaaaa ccgcggcaag
     3961 agcgacaacg tgccgagcga ggaagtggtg aagaaaatga aaaactactg gcgtcagctg
     4021 ctgaacgcca aactgatcac ccagcgtaag tttgacaacc tgaccaaagc tgagcgcggc
     4081 ggtctgagcg aactggataa agccggtttc attaagcgtc agctggtgga aacccgccag
     4141 atcaccaagc acgtggcgca gattctggac agccgcatga acaccaagta tgacgagaac
     4201 gataaactga tccgtgaagt gaaagtgatc accctgaaga gcaaactggt gagcgatttt
     4261 cgtaaagact tccagtttta caaggtgcgc gagatcaaca actaccacca cgctcacgac
     4321 gcctatctga acgcggtggt gggcaccgcc ctgatcaaga agtacccgaa gctggagagc
     4381 gagttcgtgt acggcgacta caaggtgtat gatgtgcgta agatgatcgc caaaagcgag
     4441 caggaaattg gcccgaagaa aaagcgtaaa gtgggtagca gcggcagccc gaaaaagaaa
     4501 cgcaaagtgg gtagcgacgc tctggatgac ttcgacctgg atatgctggg cagcgacgcc
     4561 ctggatgact ttggcggtgg cagcatggat agcctgctga tgaaccgtcg caaattcctg
     4621 tatcagttta agaacgtgcg ttgggctaaa ggccgtcgcg aaacctacct gtgctatgtg
     4681 gtgaaacgtc gcgatagcgc caccagcttc agcctggact ttggttacct gcgcaacaag
     4741 aacggctgcc acgtggagct gctgttcctg cgttacatta gcgactggga cctggaccca
     4801 ggccgttgct atcgcgtgac ctggtttacc agctggagcc cgtgctacga ttgcgctcgt
     4861 catgtggcgg acttcctgcg tggtaaccca aacctgagcc tgcgtatctt caccgcgcgc
     4921 ctgtactttt gcgaggaccg taaggctgag ccggaaggtc tgcgtcgcct gcaccgtgcg
     4981 ggtgtgcaga tcgcgatcat gaccttcaaa gattactttt attgctggaa caccttcgtg
     5041 gagaaccacg aacgcacctt taaggcctgg gagggtctgc acgaaaacag cgtgcgtctg
     5101 agccgccagc tgcgccgcat cctgctgcca aagcgtccgg cggctaccaa gaaagcgggc
     5161 caggctaaga aaaagaaaga ctacaaagat gacgatgaca agggtagcgg cgctaccaac
     5221 ttcagcctgc tgaagcaggc gggcgacgtg gaggaaaatc cgggcccgat ggctaaaccg
     5281 ctgagccagg aggaaagcac cctgatcgag cgcgctaccg ccaccattaa cagcatcccg
     5341 attagcgaag actatagcgt ggccagcgcc gcgctgagca gcgatggtcg tattttcacc
     5401 ggcgtgaacg tgtaccactt taccggtggc ccgtgcgccg agctggtggt gctgggcacc
     5461 gccgccgccg ccgccgccgg caacctgacc tgcatcgtgg cgattggtaa cgaaaatcgt
     5521 ggcatcctga gcccgtgcgg ccgttgccgc caggtgctgc tggacctgca tccgggcatc
     5581 aaggcgattg tgaaggatag cgacggccaa ccgaccgccg tgggtattcg tgaactgctg
     5641 ccatccggct atgtttggga gggctaagga tcc
//
