LOCUS       sequence-261032-        5958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of intradomain insertion of ABEMax (wildtype Tada monomer
            coupled with evolved Tada monomer) at position 1058 of nSpyCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S2 1058 ABEMax
FEATURES             Location/Qualifiers
     source          1..5958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3187..3207
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3223..3243
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5416..5463
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5464..5487
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5497..5553
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aagcttgcca ccatggataa aaaatatagc attggtctgg cgattggcac caactccgtg
       61 ggctgggctg tgattaccga tgaatacaag gttccgagca agaagtttaa ggtgctgggt
      121 aacaccgacc gtcacagcat caagaagaac ctgattggcg ctctgctgtt tgacagcggc
      181 gagaccgcgg aagccacccg cctgaagcgc accgcgcgtc gccgttatac ccgtcgtaag
      241 aaccgcattt gctatctgca agaaatcttc agcaacgaga tggcgaaagt ggatgacagc
      301 tttttccacc gcctggaaga gagctttctg gtggaggaag ataagaagca cgagcgtcac
      361 ccgatcttcg gtaacattgt ggacgaggtg gcctatcacg aaaagtatcc gaccatctac
      421 cacctgcgta aaaaactggt ggatagcacc gacaaagctg atctgcgcct gatctatctg
      481 gctctggcgc acatgatcaa attccgcggc cacttcctga ttgagggtga cctgaacccg
      541 gacaacagcg atgtggataa actgtttatt cagctggtgc agacctataa ccagctgttt
      601 gaagagaacc cgatcaacgc gagcggcgtg gacgcgaaag ctattctgag cgcgcgtctg
      661 agcaagagcc gtcgtctgga aaacctgatc gcgcagctgc cgggcgagaa gaaaaacggt
      721 ctgtttggca acctgattgc cctgagcctg ggtctgaccc cgaactttaa gagcaacttc
      781 gacctggcgg aggacgcgaa actgcaactg agcaaagata cctatgacga cgacctggat
      841 aacctgctgg cgcagatcgg cgatcagtat gctgacctgt ttctggcggc caaaaacctg
      901 agcgacgcga ttctgctgag cgacatcctg cgtgtgaaca ccgagattac caaagcgccg
      961 ctgagcgcca gcatgattaa gcgttacgac gaacaccacc aggacctgac cctgctgaaa
     1021 gccctggtgc gccagcagct gccggagaag tacaaagaaa tctttttcga tcagagcaaa
     1081 aacggttatg ccggttatat tgacggtggc gcgagccagg aggagtttta taagttcatt
     1141 aagccgattc tggaaaagat ggacggcacc gaggagctgc tggtgaagct gaaccgcgaa
     1201 gacctgctgc gcaaacagcg tacctttgat aacggtagca ttccgcacca gattcacctg
     1261 ggcgaactgc acgcgattct gcgtcgccag gaggacttct atccgttcct gaaggataac
     1321 cgtgagaaaa ttgagaagat tctgaccttc cgtatcccgt actatgtggg cccgctggct
     1381 cgtggtaaca gccgtttcgc ttggatgacc cgtaaaagcg aggagaccat taccccgtgg
     1441 aacttcgagg aagtggtgga caaaggtgcc agcgcgcaga gctttatcga gcgcatgacc
     1501 aacttcgaca aaaacctgcc gaacgaaaaa gtgctgccga aacacagcct gctgtatgaa
     1561 tacttcaccg tgtataacga actgaccaag gtgaagtatg tgaccgaagg tatgcgcaag
     1621 ccggcttttc tgagcggcga gcagaagaag gcgattgtgg acctgctgtt caagaccaac
     1681 cgtaaagtga ccgtgaagca gctgaaggag gactacttca agaaaattga atgctttgac
     1741 agcgtggaga tcagcggcgt ggaggaccgc ttcaacgcga gcctgggcac ctaccacgat
     1801 ctgctgaaga tcattaagga taaagatttt ctggacaacg aggaaaacga agacatcctg
     1861 gaggatattg tgctgaccct gaccctgttt gaggatcgtg aaatgattga agaacgcctg
     1921 aagacctatg ctcacctgtt cgatgataaa gtgatgaaac agctgaagcg tcgtcgttat
     1981 accggttggg gccgtctgag ccgcaaactg atcaacggca ttcgtgacaa acagagcggc
     2041 aagaccatcc tggattttct gaaaagcgat ggctttgcga accgtaactt tatgcagctg
     2101 attcacgatg atagcctgac cttcaaggag gacattcaga aggcgcaggt gagcggccag
     2161 ggtgacagcc tgcacgagca cattgcgaac ctggcgggca gcccggcgat taagaaaggc
     2221 attctgcaaa ccgtgaaagt ggtggatgaa ctggtgaaag tgatgggccg ccacaagccg
     2281 gagaacattg tgatcgagat ggcccgtgag aaccagacca cccagaaagg ccagaagaac
     2341 agccgcgagc gcatgaagcg tattgaagag ggtatcaagg agctgggcag ccagatcctg
     2401 aaggaacacc cggtggaaaa cacccagctg caaaacgaga aactgtatct gtactacctg
     2461 caaaacggcc gtgacatgta tgtggatcag gaactggaca tcaaccgcct gagcgactac
     2521 gacgtggatg cgatcgtgcc gcagagcttc ctgaaagacg acagcattga taacaaggtg
     2581 ctgacccgta gcgacaagaa ccgtggcaaa agcgataacg tgccgagcga ggaggtggtg
     2641 aaaaagatga aaaactattg gcgccagctg ctgaacgcga agctgattac ccagcgcaag
     2701 ttcgacaacc tgaccaaggc tgagcgtggt ggcctgagcg agctggacaa ggctggcttt
     2761 atcaaacgcc agctggtgga gacccgccag atcaccaagc acgtggcgca gatcctggat
     2821 agccgtatga acaccaaata cgatgagaac gataagctga tccgcgaggt gaaagtgatc
     2881 accctgaaga gcaagctggt gagcgatttt cgcaaggact tccagttcta caaggtgcgc
     2941 gaaatcaaca actaccacca cgcgcacgat gcgtatctga acgcggtggt gggcaccgct
     3001 ctgatcaaga aatatccgaa gctggagagc gagttcgtgt acggtgacta taaagtgtac
     3061 gacgtgcgca aaatgattgc caagagcgag caggagattg gtaaagctac cgctaaatat
     3121 ttcttttaca gcaacatcat gaacttcttt aagaccgaga tcaccctggc caacggtgaa
     3181 atccgtccga agaagaaacg taaggtgggt agcagcggta gcccgaaaaa gaagcgcaaa
     3241 gtgggcagcg acgcgctgga cgatttcgat ctggacatgc tgggcagcga tgcgctggat
     3301 gatttcggcg gtggcagctc tgaagtcgag tttagccacg agtattggat gaggcacgca
     3361 ctgaccctgg caaagcgagc atgggatgaa agagaagtcc ccgtgggcgc cgtgctggtg
     3421 cacaacaata gagtgatcgg agagggatgg aacaggccaa tcggccgcca cgaccctacc
     3481 gcacacgcag agatcatggc actgaggcag ggaggcctgg tcatgcagaa ttaccgcctg
     3541 atcgatgcca ccctgtatgt gacactggag ccatgcgtga tgtgcgcagg agcaatgatc
     3601 cacagcagga tcggaagagt ggtgttcgga gcacgggacg ccaagaccgg cgcagcaggc
     3661 tccctgatgg atgtgctgca ccaccccggc atgaaccacc gggtggagat cacagaggga
     3721 atcctggcag acgagtgcgc cgccctgctg agcgatttct ttagaatgcg gagacaggag
     3781 atcaaggccc agaagaaggc acagagctcc accgactctg gaggatctag cggaggatcc
     3841 tctggaagcg agacaccagg cacaagcgag tccgccacac cagagagctc cggcggctcc
     3901 tccggaggat cctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc
     3961 ctggccaaga gggcacgcga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac
     4021 aatagagtga tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat
     4081 gccgaaatta tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac
     4141 gccaccctgt acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct
     4201 aggatcggcc gcgtggtgtt tggcgtgagg aacgcaaaaa ccggcgccgc aggctccctg
     4261 atggacgtgc tgcactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg
     4321 gcagatgaat gtgccgccct gctgtgctat ttctttcgga tgcctagaca ggtgttcaat
     4381 gctcagaaga aggcccagag ctccaccgac ggcggtagcg gcggtagcgg tggtagcggc
     4441 ggcagcggtg gtagcggtgg cagcggtggt cgcaaacgcc cgctgatcga aaccaacggc
     4501 gaaaccggtg aaatcgtgtg ggataagggt cgcgatttcg cgaccgtgcg caaggtgctg
     4561 agcatgccgc aggtgaacat cgtgaagaag accgaagtgc agaccggcgg ttttagcaag
     4621 gaaagcatcc tgccgaagcg caacagcgat aagctgatcg cgcgtaagaa ggactgggac
     4681 ccgaaaaagt atggtggctt cgacagcccg accgtggcgt acagcgtgct ggtggtggcc
     4741 aaggtggaaa agggcaaaag caaaaaactg aaaagcgtga aggagctgct gggtattacc
     4801 attatggaac gtagcagctt tgagaagaac ccgatcgact tcctggaggc gaagggttac
     4861 aaggaagtga agaaagatct gatcatcaaa ctgccgaagt acagcctgtt tgagctggaa
     4921 aacggccgta agcgtatgct ggcgagcgcg ggtgagctgc aaaaaggtaa cgagctggcg
     4981 ctgccgagca agtacgtgaa ctttctgtac ctggcgagcc actatgaaaa actgaaaggc
     5041 agcccggagg acaacgagca gaagcagctg tttgtggagc agcacaagca ctatctggac
     5101 gaaatcatcg aacagatcag cgagttcagc aaacgcgtga ttctggccga cgccaacctg
     5161 gacaaagtgc tgagcgccta caacaagcac cgcgataaac cgattcgcga acaggccgag
     5221 aacatcattc acctgtttac cctgaccaac ctgggcgcgc cggcggcgtt caaatacttt
     5281 gataccacca ttgatcgcaa acgctacacc agcaccaagg aagtgctgga tgcgaccctg
     5341 atccaccaga gcattaccgg tctgtacgaa acccgcatcg atctgagcca gctgggcggc
     5401 gatagcggtg gtagcaagcg tccggcggcg accaaaaagg ccggccaggc gaagaagaag
     5461 aaggactaca aggatgacga tgacaaaggc agcggcgcca ccaacttcag cctgctgaag
     5521 caggccggtg acgtggaaga aaaccccggc ccgatggcga agccgctgag ccaggaggaa
     5581 agcaccctga ttgagcgcgc caccgcgacc atcaacagca ttccgattag cgaagactac
     5641 agcgtggcga gcgccgctct gagcagcgat ggccgtatct tcaccggcgt gaacgtgtac
     5701 cactttaccg gcggtccgtg cgcggaactg gtggtgctgg gcaccgcggc ggctgcggct
     5761 gcgggtaacc tgacctgcat cgtggccatt ggtaacgaaa accgcggcat tctgagcccg
     5821 tgcggccgtt gccgccaggt gctgctggac ctgcaccccg gtatcaaagc gattgtgaaa
     5881 gatagcgatg gtcaaccgac cgctgtgggt atccgtgaac tgctgccatc cggctatgtt
     5941 tgggagggct aaggtacc
//
