LOCUS       sequence-261033-        5964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1029 encoding ABEMax (wildtype Tada monomer coupled with evolved 
            Tada monomer) at the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 CP ABEMax strategy 1
FEATURES             Location/Qualifiers
     source          1..5964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1108..1128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1144..1164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5428..5475
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5476..5499
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5509..5565
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggtaccgcca ccatgtctga agtcgagttt agccacgagt attggatgag gcacgcactg
       61 accctggcaa agcgagcatg ggatgaaaga gaagtccccg tgggcgccgt gctggtgcac
      121 aacaatagag tgatcggaga gggatggaac aggccaatcg gccgccacga ccctaccgca
      181 cacgcagaga tcatggcact gaggcaggga ggcctggtca tgcagaatta ccgcctgatc
      241 gatgccaccc tgtatgtgac actggagcca tgcgtgatgt gcgcaggagc aatgatccac
      301 agcaggatcg gaagagtggt gttcggagca cgggacgcca agaccggcgc agcaggctcc
      361 ctgatggatg tgctgcacca ccccggcatg aaccaccggg tggagatcac agagggaatc
      421 ctggcagacg agtgcgccgc cctgctgagc gatttcttta gaatgcggag acaggagatc
      481 aaggcccaga agaaggcaca gagctccacc gactctggag gatctagcgg aggatcctct
      541 ggaagcgaga caccaggcac aagcgagtcc gccacaccag agagctccgg cggctcctcc
      601 ggaggatcct ctgaggtgga gttttcccac gagtactgga tgagacatgc cctgaccctg
      661 gccaagaggg cacgcgatga gagggaggtg cctgtgggag ccgtgctggt gctgaacaat
      721 agagtgatcg gcgagggctg gaacagagcc atcggcctgc acgacccaac agcccatgcc
      781 gaaattatgg ccctgagaca gggcggcctg gtcatgcaga actacagact gattgacgcc
      841 accctgtacg tgacattcga gccttgcgtg atgtgcgccg gcgccatgat ccactctagg
      901 atcggccgcg tggtgtttgg cgtgaggaac gcaaaaaccg gcgccgcagg ctccctgatg
      961 gacgtgctgc actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg aatcctggca
     1021 gatgaatgtg ccgccctgct gtgctatttc tttcggatgc ctagacaggt gttcaatgct
     1081 cagaagaagg cccagagctc caccgacccg aagaaaaagc gtaaagtggg tagcagcggc
     1141 agcccgaaaa agaaacgcaa agtgggtagc gacgctctgg atgacttcga cctggatatg
     1201 ctgggcagcg acgccctgga tgactttggc ggtggcagca taggcaaggc aaccgctaag
     1261 tatttttttt acagcaacat catgaacttc tttaagaccg agattaccct ggccaacggt
     1321 gaaatccgta aacgcccgct gattgaaacc aacggtgaaa ccggcgaaat cgtgtgggac
     1381 aagggccgtg attttgcgac cgtgcgcaaa gtgctgtcca tgccgcaggt gaacattgtg
     1441 aagaaaaccg aggtgcagac cggcggtttc agcaaggaaa gcattctgcc gaaacgtaac
     1501 agcgacaagc tgatcgcccg caagaaagat tgggacccga agaaatacgg cggtttcgat
     1561 agcccgaccg tggcttatag cgtgctggtg gtggccaaag tggagaaggg taaaagcaag
     1621 aaactgaaga gcgtgaaaga actgctgggc atcaccatta tggagcgtag cagcttcgaa
     1681 aagaacccga tcgactttct ggaggcgaag ggttacaagg aagtgaagaa agatctgatc
     1741 attaagctgc cgaaatacag cctgtttgag ctggaaaatg gtcgtaaacg tatgctggcc
     1801 agcgccggcg agctgcaaaa gggcaacgaa ctggcgctgc cgagcaaata tgtgaacttc
     1861 ctgtacctgg ccagccacta tgagaagctg aaaggtagcc cggaggacaa cgaacagaag
     1921 cagctgtttg tggagcagca caaacactat ctggatgaga tcattgaaca gatcagcgag
     1981 ttcagcaagc gtgtgattct ggcggacgct aacctggata aagtgctgag cgcctacaac
     2041 aaacaccgtg acaagccgat ccgcgagcag gcggaaaaca tcattcacct gttcaccctg
     2101 accaacctgg gtgcgccggc cgcgttcaag tactttgaca ccaccattga tcgtaaacgc
     2161 tataccagca ccaaggaggt gctggatgct accctgatcc accagagcat taccggcctg
     2221 tacgaaaccc gtattgatct gagccagctg ggcggtgacg gcggtagcgg cggtagcggc
     2281 ggtagcggcg gtagcggcgg tagcggcggt agcggcggtg acaagaaata tagcatcggt
     2341 ctggcgattg gcaccaacag cgtgggttgg gctgtgatca ccgatgagta caaggtgccg
     2401 agcaagaagt tcaaggtgct gggcaacacc gaccgccaca gcatcaagaa aaacctgatt
     2461 ggtgccctgc tgtttgatag cggtgaaacc gccgaggcca cccgtctgaa gcgcaccgcc
     2521 cgtcgccgtt acacccgtcg taaaaaccgt atctgctacc tgcaagagat tttcagcaac
     2581 gaaatggcca aagtggacga tagcttcttt caccgcctgg aggagagctt cctggtggag
     2641 gaagacaaga aacacgagcg tcacccgatc tttggcaaca ttgtggatga ggtggcttac
     2701 cacgaaaagt atccgaccat ctaccacctg cgtaagaaac tggtggatag caccgacaag
     2761 gccgatctgc gcctgatcta tctggctctg gcccacatga tcaagttccg tggtcacttt
     2821 ctgatcgagg gcgacctgaa cccggataac agcgatgtgg acaagctgtt catccagctg
     2881 gtgcagacct acaaccagct gtttgaggaa aacccgatta acgccagcgg tgtggacgcg
     2941 aaggctatcc tgagcgcgcg tctgagcaaa agccgccgtc tggagaacct gatcgcgcag
     3001 ctgccgggtg aaaagaaaaa cggtctgttt ggcaacctga ttgctctgag cctgggcctg
     3061 accccgaact tcaaaagcaa ctttgacctg gcggaggatg ctaagctgca actgagcaag
     3121 gatacctatg acgatgacct ggacaacctg ctggcgcaga tcggcgacca gtacgctgat
     3181 ctgttcctgg ctgccaagaa cctgagcgat gcgatcctgc tgagcgacat tctgcgtgtg
     3241 aacaccgaga ttaccaaggc cccgctgagc gcgagcatga tcaaacgcta tgatgaacac
     3301 caccaggacc tgaccctgct gaaagctctg gtgcgtcagc agctgccgga gaagtacaaa
     3361 gaaatcttct ttgaccagag caagaacggt tacgccggct atattgatgg cggtgcgagc
     3421 caggaggagt tctacaagtt tatcaaaccg attctggaga aaatggacgg caccgaggaa
     3481 ctgctggtga agctgaaccg tgaagatctg ctgcgtaaac agcgcacctt cgacaacggt
     3541 agcatcccgc accagattca cctgggtgag ctgcacgcca tcctgcgccg tcaggaagat
     3601 ttctacccgt ttctgaagga caaccgtgag aagatcgaaa aaattctgac cttccgcatt
     3661 ccgtactatg tgggtccgct ggctcgtggc aacagccgct ttgcctggat gacccgcaag
     3721 agcgaggaaa ccatcacccc gtggaacttc gaggaagtgg tggacaaagg cgccagcgcg
     3781 cagagcttca ttgagcgtat gaccaacttt gataagaacc tgccgaacga aaaagtgctg
     3841 ccgaagcaca gcctgctgta cgagtatttc accgtgtaca acgaactgac caaggtgaaa
     3901 tatgtgaccg agggtatgcg caagccggcc ttcctgagcg gcgaacagaa gaaagcgatc
     3961 gtggacctgc tgtttaaaac caaccgtaag gtgaccgtga agcagctgaa agaggattat
     4021 ttcaagaaaa ttgaatgctt tgacagcgtg gagatcagcg gtgtggaaga tcgttttaac
     4081 gcgagcctgg gcacctacca cgacctgctg aaaatcatta aggataaaga cttcctggat
     4141 aacgaggaaa acgaggacat cctggaagat attgtgctga ccctgaccct gtttgaggac
     4201 cgtgaaatga tcgaggaacg cctgaagacc tatgcccacc tgttcgatga caaagtgatg
     4261 aaacagctga agcgccgtcg ctacaccggt tggggccgtc tgagccgcaa actgatcaac
     4321 ggtattcgcg ataaacagag cggcaagacc atcctggatt ttctgaagag cgacggtttc
     4381 gcgaaccgta actttatgca gctgattcac gatgacagcc tgaccttcaa agaggacatc
     4441 cagaaggcgc aggtgagcgg tcagggcgat agcctgcacg aacatattgc taacctggcc
     4501 ggtagcccgg ccatcaagaa gggcatcctg caaaccgtga aggtggtgga cgagctggtg
     4561 aaagtgatgg gtcgtcacaa gccggaaaac atcgtgattg agatggcccg cgaaaaccag
     4621 accacccaga aaggccagaa gaacagccgt gagcgcatga agcgtatcga ggaaggcatc
     4681 aaggaactgg gcagccagat cctgaaggag cacccggtgg aaaacaccca gctgcaaaac
     4741 gagaaactgt acctgtacta cctgcaaaac ggtcgtgaca tgtatgtgga tcaggaactg
     4801 gacattaacc gcctgagcga ttatgacgtg gatgcgatcg tgccgcagag cttcctgaag
     4861 gatgacagca ttgacaacaa agtgctgacc cgtagcgata aaaaccgcgg caagagcgac
     4921 aacgtgccga gcgaggaagt ggtgaagaaa atgaaaaact actggcgtca gctgctgaac
     4981 gccaaactga tcacccagcg taagtttgac aacctgacca aagctgagcg cggcggtctg
     5041 agcgaactgg ataaagccgg tttcattaag cgtcagctgg tggaaacccg ccagatcacc
     5101 aagcacgtgg cgcagattct ggacagccgc atgaacacca agtatgacga gaacgataaa
     5161 ctgatccgtg aagtgaaagt gatcaccctg aagagcaaac tggtgagcga ttttcgtaaa
     5221 gacttccagt tttacaaggt gcgcgagatc aacaactacc accacgctca cgacgcctat
     5281 ctgaacgcgg tggtgggcac cgccctgatc aagaagtacc cgaagctgga gagcgagttc
     5341 gtgtacggcg actacaaggt gtatgatgtg cgtaagatga tcgccaaaag cgagcaggaa
     5401 attggcggcg gcgatagcgg tggtagcaag cgtccggcgg ctaccaagaa agcgggccag
     5461 gctaagaaaa agaaagacta caaagatgac gatgacaagg gtagcggcgc taccaacttc
     5521 agcctgctga agcaggcggg cgacgtggag gaaaatccgg gcccgatggc taaaccgctg
     5581 agccaggagg aaagcaccct gatcgagcgc gctaccgcca ccattaacag catcccgatt
     5641 agcgaagact atagcgtggc cagcgccgcg ctgagcagcg atggtcgtat tttcaccggc
     5701 gtgaacgtgt accactttac cggtggcccg tgcgccgagc tggtggtgct gggcaccgcc
     5761 gccgccgccg ccgccggcaa cctgacctgc atcgtggcga ttggtaacga aaatcgtggc
     5821 atcctgagcc cgtgcggccg ttgccgccag gtgctgctgg acctgcatcc gggcatcaag
     5881 gcgattgtga aggatagcga cggccaaccg accgccgtgg gtattcgtga actgctgcca
     5941 tccggctatg tttgggaggg ctaa
//
