LOCUS       sequence-261035-        5940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1029 encoding ABEMax (wildtype Tada monomer coupled with evolved 
            Tada monomer) at the C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 CP ABEMax strategy 3
FEATURES             Location/Qualifiers
     source          1..5940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4183..4203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4219..4239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5428..5475
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5485..5541
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggtaccgcca ccatgatagg caaggcaacc gctaagtatt ttttttacag caacatcatg
       61 aacttcttta agaccgagat taccctggcc aacggtgaaa tccgtaaacg cccgctgatt
      121 gaaaccaacg gtgaaaccgg cgaaatcgtg tgggacaagg gccgtgattt tgcgaccgtg
      181 cgcaaagtgc tgtccatgcc gcaggtgaac attgtgaaga aaaccgaggt gcagaccggc
      241 ggtttcagca aggaaagcat tctgccgaaa cgtaacagcg acaagctgat cgcccgcaag
      301 aaagattggg acccgaagaa atacggcggt ttcgatagcc cgaccgtggc ttatagcgtg
      361 ctggtggtgg ccaaagtgga gaagggtaaa agcaagaaac tgaagagcgt gaaagaactg
      421 ctgggcatca ccattatgga gcgtagcagc ttcgaaaaga acccgatcga ctttctggag
      481 gcgaagggtt acaaggaagt gaagaaagat ctgatcatta agctgccgaa atacagcctg
      541 tttgagctgg aaaatggtcg taaacgtatg ctggccagcg ccggcgagct gcaaaagggc
      601 aacgaactgg cgctgccgag caaatatgtg aacttcctgt acctggccag ccactatgag
      661 aagctgaaag gtagcccgga ggacaacgaa cagaagcagc tgtttgtgga gcagcacaaa
      721 cactatctgg atgagatcat tgaacagatc agcgagttca gcaagcgtgt gattctggcg
      781 gacgctaacc tggataaagt gctgagcgcc tacaacaaac accgtgacaa gccgatccgc
      841 gagcaggcgg aaaacatcat tcacctgttc accctgacca acctgggtgc gccggccgcg
      901 ttcaagtact ttgacaccac cattgatcgt aaacgctata ccagcaccaa ggaggtgctg
      961 gatgctaccc tgatccacca gagcattacc ggcctgtacg aaacccgtat tgatctgagc
     1021 cagctgggcg gtgacggcgg tagcggcggt agcggcggta gcggcggtag cggcggtagc
     1081 ggcggtagcg gcggtgacaa gaaatatagc atcggtctgg cgattggcac caacagcgtg
     1141 ggttgggctg tgatcaccga tgagtacaag gtgccgagca agaagttcaa ggtgctgggc
     1201 aacaccgacc gccacagcat caagaaaaac ctgattggtg ccctgctgtt tgatagcggt
     1261 gaaaccgccg aggccacccg tctgaagcgc accgcccgtc gccgttacac ccgtcgtaaa
     1321 aaccgtatct gctacctgca agagattttc agcaacgaaa tggccaaagt ggacgatagc
     1381 ttctttcacc gcctggagga gagcttcctg gtggaggaag acaagaaaca cgagcgtcac
     1441 ccgatctttg gcaacattgt ggatgaggtg gcttaccacg aaaagtatcc gaccatctac
     1501 cacctgcgta agaaactggt ggatagcacc gacaaggccg atctgcgcct gatctatctg
     1561 gctctggccc acatgatcaa gttccgtggt cactttctga tcgagggcga cctgaacccg
     1621 gataacagcg atgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttt
     1681 gaggaaaacc cgattaacgc cagcggtgtg gacgcgaagg ctatcctgag cgcgcgtctg
     1741 agcaaaagcc gccgtctgga gaacctgatc gcgcagctgc cgggtgaaaa gaaaaacggt
     1801 ctgtttggca acctgattgc tctgagcctg ggcctgaccc cgaacttcaa aagcaacttt
     1861 gacctggcgg aggatgctaa gctgcaactg agcaaggata cctatgacga tgacctggac
     1921 aacctgctgg cgcagatcgg cgaccagtac gctgatctgt tcctggctgc caagaacctg
     1981 agcgatgcga tcctgctgag cgacattctg cgtgtgaaca ccgagattac caaggccccg
     2041 ctgagcgcga gcatgatcaa acgctatgat gaacaccacc aggacctgac cctgctgaaa
     2101 gctctggtgc gtcagcagct gccggagaag tacaaagaaa tcttctttga ccagagcaag
     2161 aacggttacg ccggctatat tgatggcggt gcgagccagg aggagttcta caagtttatc
     2221 aaaccgattc tggagaaaat ggacggcacc gaggaactgc tggtgaagct gaaccgtgaa
     2281 gatctgctgc gtaaacagcg caccttcgac aacggtagca tcccgcacca gattcacctg
     2341 ggtgagctgc acgccatcct gcgccgtcag gaagatttct acccgtttct gaaggacaac
     2401 cgtgagaaga tcgaaaaaat tctgaccttc cgcattccgt actatgtggg tccgctggct
     2461 cgtggcaaca gccgctttgc ctggatgacc cgcaagagcg aggaaaccat caccccgtgg
     2521 aacttcgagg aagtggtgga caaaggcgcc agcgcgcaga gcttcattga gcgtatgacc
     2581 aactttgata agaacctgcc gaacgaaaaa gtgctgccga agcacagcct gctgtacgag
     2641 tatttcaccg tgtacaacga actgaccaag gtgaaatatg tgaccgaggg tatgcgcaag
     2701 ccggccttcc tgagcggcga acagaagaaa gcgatcgtgg acctgctgtt taaaaccaac
     2761 cgtaaggtga ccgtgaagca gctgaaagag gattatttca agaaaattga atgctttgac
     2821 agcgtggaga tcagcggtgt ggaagatcgt tttaacgcga gcctgggcac ctaccacgac
     2881 ctgctgaaaa tcattaagga taaagacttc ctggataacg aggaaaacga ggacatcctg
     2941 gaagatattg tgctgaccct gaccctgttt gaggaccgtg aaatgatcga ggaacgcctg
     3001 aagacctatg cccacctgtt cgatgacaaa gtgatgaaac agctgaagcg ccgtcgctac
     3061 accggttggg gccgtctgag ccgcaaactg atcaacggta ttcgcgataa acagagcggc
     3121 aagaccatcc tggattttct gaagagcgac ggtttcgcga accgtaactt tatgcagctg
     3181 attcacgatg acagcctgac cttcaaagag gacatccaga aggcgcaggt gagcggtcag
     3241 ggcgatagcc tgcacgaaca tattgctaac ctggccggta gcccggccat caagaagggc
     3301 atcctgcaaa ccgtgaaggt ggtggacgag ctggtgaaag tgatgggtcg tcacaagccg
     3361 gaaaacatcg tgattgagat ggcccgcgaa aaccagacca cccagaaagg ccagaagaac
     3421 agccgtgagc gcatgaagcg tatcgaggaa ggcatcaagg aactgggcag ccagatcctg
     3481 aaggagcacc cggtggaaaa cacccagctg caaaacgaga aactgtacct gtactacctg
     3541 caaaacggtc gtgacatgta tgtggatcag gaactggaca ttaaccgcct gagcgattat
     3601 gacgtggatg cgatcgtgcc gcagagcttc ctgaaggatg acagcattga caacaaagtg
     3661 ctgacccgta gcgataaaaa ccgcggcaag agcgacaacg tgccgagcga ggaagtggtg
     3721 aagaaaatga aaaactactg gcgtcagctg ctgaacgcca aactgatcac ccagcgtaag
     3781 tttgacaacc tgaccaaagc tgagcgcggc ggtctgagcg aactggataa agccggtttc
     3841 attaagcgtc agctggtgga aacccgccag atcaccaagc acgtggcgca gattctggac
     3901 agccgcatga acaccaagta tgacgagaac gataaactga tccgtgaagt gaaagtgatc
     3961 accctgaaga gcaaactggt gagcgatttt cgtaaagact tccagtttta caaggtgcgc
     4021 gagatcaaca actaccacca cgctcacgac gcctatctga acgcggtggt gggcaccgcc
     4081 ctgatcaaga agtacccgaa gctggagagc gagttcgtgt acggcgacta caaggtgtat
     4141 gatgtgcgta agatgatcgc caaaagcgag caggaaattg gcccgaagaa aaagcgtaaa
     4201 gtgggtagca gcggcagccc gaaaaagaaa cgcaaagtgg gtagcgacgc tctggatgac
     4261 ttcgacctgg atatgctggg cagcgacgcc ctggatgact ttggcggtgg cagctctgaa
     4321 gtcgagttta gccacgagta ttggatgagg cacgcactga ccctggcaaa gcgagcatgg
     4381 gatgaaagag aagtccccgt gggcgccgtg ctggtgcaca acaatagagt gatcggagag
     4441 ggatggaaca ggccaatcgg ccgccacgac cctaccgcac acgcagagat catggcactg
     4501 aggcagggag gcctggtcat gcagaattac cgcctgatcg atgccaccct gtatgtgaca
     4561 ctggagccat gcgtgatgtg cgcaggagca atgatccaca gcaggatcgg aagagtggtg
     4621 ttcggagcac gggacgccaa gaccggcgca gcaggctccc tgatggatgt gctgcaccac
     4681 cccggcatga accaccgggt ggagatcaca gagggaatcc tggcagacga gtgcgccgcc
     4741 ctgctgagcg atttctttag aatgcggaga caggagatca aggcccagaa gaaggcacag
     4801 agctccaccg actctggagg atctagcgga ggatcctctg gaagcgagac accaggcaca
     4861 agcgagtccg ccacaccaga gagctccggc ggctcctccg gaggatcctc tgaggtggag
     4921 ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc acgcgatgag
     4981 agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg
     5041 aacagagcca tcggcctgca cgacccaaca gcccatgccg aaattatggc cctgagacag
     5101 ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgt gacattcgag
     5161 ccttgcgtga tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc
     5221 gtgaggaacg caaaaaccgg cgccgcaggc tccctgatgg acgtgctgca ctaccccggc
     5281 atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg
     5341 tgctatttct ttcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc
     5401 accgacggcg gcgatagcgg tggtagcaag cgtccggcgg ctaccaagaa agcgggccag
     5461 gctaagaaaa agaaaggtag cggcgctacc aacttcagcc tgctgaagca ggcgggcgac
     5521 gtggaggaaa atccgggccc gatggctaaa ccgctgagcc aggaggaaag caccctgatc
     5581 gagcgcgcta ccgccaccat taacagcatc ccgattagcg aagactatag cgtggccagc
     5641 gccgcgctga gcagcgatgg tcgtattttc accggcgtga acgtgtacca ctttaccggt
     5701 ggcccgtgcg ccgagctggt ggtgctgggc accgccgccg ccgccgccgc cggcaacctg
     5761 acctgcatcg tggcgattgg taacgaaaat cgtggcatcc tgagcccgtg cggccgttgc
     5821 cgccaggtgc tgctggacct gcatccgggc atcaaggcga ttgtgaagga tagcgacggc
     5881 caaccgaccg ccgtgggtat tcgtgaactg ctgccatccg gctatgtttg ggagggctaa
//
