LOCUS       sequence-261036-        5370 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1029 encoding miniABEMax (evolved Tada monomer with V82G) at the 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 miniABEMax V82G strategy1
FEATURES             Location/Qualifiers
     source          1..5370
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             514..534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             550..570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4834..4881
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4882..4905
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4915..4971
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggtaccgcca ccatgtctga ggtggagttt tcccacgagt actggatgag acatgccctg
       61 accctggcca agagggcacg cgatgagagg gaggtgcctg tgggagccgt gctggtgctg
      121 aacaatagag tgatcggcga gggctggaac agagccatcg gcctgcacga cccaacagcc
      181 catgccgaaa ttatggccct gagacagggc ggcctggtca tgcagaacta cagactgatt
      241 gacgccaccc tgtacggcac attcgagcct tgcgtgatgt gcgccggcgc catgatccac
      301 tctaggatcg gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc cgcaggctcc
      361 ctgatggacg tgctgcacta ccccggcatg aatcaccgcg tcgaaattac cgagggaatc
      421 ctggcagatg aatgtgccgc cctgctgtgc tatttctttc ggatgcctag acaggtgttc
      481 aatgctcaga agaaggccca gagctccacc gacccgaaga aaaagcgtaa agtgggtagc
      541 agcggcagcc cgaaaaagaa acgcaaagtg ggtagcgacg ctctggatga cttcgacctg
      601 gatatgctgg gcagcgacgc cctggatgac tttggcggtg gcagcatagg caaggcaacc
      661 gctaagtatt ttttttacag caacatcatg aacttcttta agaccgagat taccctggcc
      721 aacggtgaaa tccgtaaacg cccgctgatt gaaaccaacg gtgaaaccgg cgaaatcgtg
      781 tgggacaagg gccgtgattt tgcgaccgtg cgcaaagtgc tgtccatgcc gcaggtgaac
      841 attgtgaaga aaaccgaggt gcagaccggc ggtttcagca aggaaagcat tctgccgaaa
      901 cgtaacagcg acaagctgat cgcccgcaag aaagattggg acccgaagaa atacggcggt
      961 ttcgatagcc cgaccgtggc ttatagcgtg ctggtggtgg ccaaagtgga gaagggtaaa
     1021 agcaagaaac tgaagagcgt gaaagaactg ctgggcatca ccattatgga gcgtagcagc
     1081 ttcgaaaaga acccgatcga ctttctggag gcgaagggtt acaaggaagt gaagaaagat
     1141 ctgatcatta agctgccgaa atacagcctg tttgagctgg aaaatggtcg taaacgtatg
     1201 ctggccagcg ccggcgagct gcaaaagggc aacgaactgg cgctgccgag caaatatgtg
     1261 aacttcctgt acctggccag ccactatgag aagctgaaag gtagcccgga ggacaacgaa
     1321 cagaagcagc tgtttgtgga gcagcacaaa cactatctgg atgagatcat tgaacagatc
     1381 agcgagttca gcaagcgtgt gattctggcg gacgctaacc tggataaagt gctgagcgcc
     1441 tacaacaaac accgtgacaa gccgatccgc gagcaggcgg aaaacatcat tcacctgttc
     1501 accctgacca acctgggtgc gccggccgcg ttcaagtact ttgacaccac cattgatcgt
     1561 aaacgctata ccagcaccaa ggaggtgctg gatgctaccc tgatccacca gagcattacc
     1621 ggcctgtacg aaacccgtat tgatctgagc cagctgggcg gtgacggcgg tagcggcggt
     1681 agcggcggta gcggcggtag cggcggtagc ggcggtagcg gcggtgacaa gaaatatagc
     1741 atcggtctgg cgattggcac caacagcgtg ggttgggctg tgatcaccga tgagtacaag
     1801 gtgccgagca agaagttcaa ggtgctgggc aacaccgacc gccacagcat caagaaaaac
     1861 ctgattggtg ccctgctgtt tgatagcggt gaaaccgccg aggccacccg tctgaagcgc
     1921 accgcccgtc gccgttacac ccgtcgtaaa aaccgtatct gctacctgca agagattttc
     1981 agcaacgaaa tggccaaagt ggacgatagc ttctttcacc gcctggagga gagcttcctg
     2041 gtggaggaag acaagaaaca cgagcgtcac ccgatctttg gcaacattgt ggatgaggtg
     2101 gcttaccacg aaaagtatcc gaccatctac cacctgcgta agaaactggt ggatagcacc
     2161 gacaaggccg atctgcgcct gatctatctg gctctggccc acatgatcaa gttccgtggt
     2221 cactttctga tcgagggcga cctgaacccg gataacagcg atgtggacaa gctgttcatc
     2281 cagctggtgc agacctacaa ccagctgttt gaggaaaacc cgattaacgc cagcggtgtg
     2341 gacgcgaagg ctatcctgag cgcgcgtctg agcaaaagcc gccgtctgga gaacctgatc
     2401 gcgcagctgc cgggtgaaaa gaaaaacggt ctgtttggca acctgattgc tctgagcctg
     2461 ggcctgaccc cgaacttcaa aagcaacttt gacctggcgg aggatgctaa gctgcaactg
     2521 agcaaggata cctatgacga tgacctggac aacctgctgg cgcagatcgg cgaccagtac
     2581 gctgatctgt tcctggctgc caagaacctg agcgatgcga tcctgctgag cgacattctg
     2641 cgtgtgaaca ccgagattac caaggccccg ctgagcgcga gcatgatcaa acgctatgat
     2701 gaacaccacc aggacctgac cctgctgaaa gctctggtgc gtcagcagct gccggagaag
     2761 tacaaagaaa tcttctttga ccagagcaag aacggttacg ccggctatat tgatggcggt
     2821 gcgagccagg aggagttcta caagtttatc aaaccgattc tggagaaaat ggacggcacc
     2881 gaggaactgc tggtgaagct gaaccgtgaa gatctgctgc gtaaacagcg caccttcgac
     2941 aacggtagca tcccgcacca gattcacctg ggtgagctgc acgccatcct gcgccgtcag
     3001 gaagatttct acccgtttct gaaggacaac cgtgagaaga tcgaaaaaat tctgaccttc
     3061 cgcattccgt actatgtggg tccgctggct cgtggcaaca gccgctttgc ctggatgacc
     3121 cgcaagagcg aggaaaccat caccccgtgg aacttcgagg aagtggtgga caaaggcgcc
     3181 agcgcgcaga gcttcattga gcgtatgacc aactttgata agaacctgcc gaacgaaaaa
     3241 gtgctgccga agcacagcct gctgtacgag tatttcaccg tgtacaacga actgaccaag
     3301 gtgaaatatg tgaccgaggg tatgcgcaag ccggccttcc tgagcggcga acagaagaaa
     3361 gcgatcgtgg acctgctgtt taaaaccaac cgtaaggtga ccgtgaagca gctgaaagag
     3421 gattatttca agaaaattga atgctttgac agcgtggaga tcagcggtgt ggaagatcgt
     3481 tttaacgcga gcctgggcac ctaccacgac ctgctgaaaa tcattaagga taaagacttc
     3541 ctggataacg aggaaaacga ggacatcctg gaagatattg tgctgaccct gaccctgttt
     3601 gaggaccgtg aaatgatcga ggaacgcctg aagacctatg cccacctgtt cgatgacaaa
     3661 gtgatgaaac agctgaagcg ccgtcgctac accggttggg gccgtctgag ccgcaaactg
     3721 atcaacggta ttcgcgataa acagagcggc aagaccatcc tggattttct gaagagcgac
     3781 ggtttcgcga accgtaactt tatgcagctg attcacgatg acagcctgac cttcaaagag
     3841 gacatccaga aggcgcaggt gagcggtcag ggcgatagcc tgcacgaaca tattgctaac
     3901 ctggccggta gcccggccat caagaagggc atcctgcaaa ccgtgaaggt ggtggacgag
     3961 ctggtgaaag tgatgggtcg tcacaagccg gaaaacatcg tgattgagat ggcccgcgaa
     4021 aaccagacca cccagaaagg ccagaagaac agccgtgagc gcatgaagcg tatcgaggaa
     4081 ggcatcaagg aactgggcag ccagatcctg aaggagcacc cggtggaaaa cacccagctg
     4141 caaaacgaga aactgtacct gtactacctg caaaacggtc gtgacatgta tgtggatcag
     4201 gaactggaca ttaaccgcct gagcgattat gacgtggatg cgatcgtgcc gcagagcttc
     4261 ctgaaggatg acagcattga caacaaagtg ctgacccgta gcgataaaaa ccgcggcaag
     4321 agcgacaacg tgccgagcga ggaagtggtg aagaaaatga aaaactactg gcgtcagctg
     4381 ctgaacgcca aactgatcac ccagcgtaag tttgacaacc tgaccaaagc tgagcgcggc
     4441 ggtctgagcg aactggataa agccggtttc attaagcgtc agctggtgga aacccgccag
     4501 atcaccaagc acgtggcgca gattctggac agccgcatga acaccaagta tgacgagaac
     4561 gataaactga tccgtgaagt gaaagtgatc accctgaaga gcaaactggt gagcgatttt
     4621 cgtaaagact tccagtttta caaggtgcgc gagatcaaca actaccacca cgctcacgac
     4681 gcctatctga acgcggtggt gggcaccgcc ctgatcaaga agtacccgaa gctggagagc
     4741 gagttcgtgt acggcgacta caaggtgtat gatgtgcgta agatgatcgc caaaagcgag
     4801 caggaaattg gcggcggcga tagcggtggt agcaagcgtc cggcggctac caagaaagcg
     4861 ggccaggcta agaaaaagaa agactacaaa gatgacgatg acaagggtag cggcgctacc
     4921 aacttcagcc tgctgaagca ggcgggcgac gtggaggaaa atccgggccc gatggctaaa
     4981 ccgctgagcc aggaggaaag caccctgatc gagcgcgcta ccgccaccat taacagcatc
     5041 ccgattagcg aagactatag cgtggccagc gccgcgctga gcagcgatgg tcgtattttc
     5101 accggcgtga acgtgtacca ctttaccggt ggcccgtgcg ccgagctggt ggtgctgggc
     5161 accgccgccg ccgccgccgc cggcaacctg acctgcatcg tggcgattgg taacgaaaat
     5221 cgtggcatcc tgagcccgtg cggccgttgc cgccaggtgc tgctggacct gcatccgggc
     5281 atcaaggcga ttgtgaagga tagcgacggc caaccgaccg ccgtgggtat tcgtgaactg
     5341 ctgccatccg gctatgtttg ggagggctaa
//
