LOCUS       sequence-261037-        5364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of intradomain insertion of miniABEMax (evolved Tada 
            monomer with V82G) at position 1058 of nSpyCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S2 1058 V82G ABEMax
FEATURES             Location/Qualifiers
     source          1..5364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3187..3207
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3223..3243
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4822..4869
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4870..4893
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4903..4959
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aagcttgcca ccatggataa aaaatatagc attggtctgg cgattggcac caactccgtg
       61 ggctgggctg tgattaccga tgaatacaag gttccgagca agaagtttaa ggtgctgggt
      121 aacaccgacc gtcacagcat caagaagaac ctgattggcg ctctgctgtt tgacagcggc
      181 gagaccgcgg aagccacccg cctgaagcgc accgcgcgtc gccgttatac ccgtcgtaag
      241 aaccgcattt gctatctgca agaaatcttc agcaacgaga tggcgaaagt ggatgacagc
      301 tttttccacc gcctggaaga gagctttctg gtggaggaag ataagaagca cgagcgtcac
      361 ccgatcttcg gtaacattgt ggacgaggtg gcctatcacg aaaagtatcc gaccatctac
      421 cacctgcgta aaaaactggt ggatagcacc gacaaagctg atctgcgcct gatctatctg
      481 gctctggcgc acatgatcaa attccgcggc cacttcctga ttgagggtga cctgaacccg
      541 gacaacagcg atgtggataa actgtttatt cagctggtgc agacctataa ccagctgttt
      601 gaagagaacc cgatcaacgc gagcggcgtg gacgcgaaag ctattctgag cgcgcgtctg
      661 agcaagagcc gtcgtctgga aaacctgatc gcgcagctgc cgggcgagaa gaaaaacggt
      721 ctgtttggca acctgattgc cctgagcctg ggtctgaccc cgaactttaa gagcaacttc
      781 gacctggcgg aggacgcgaa actgcaactg agcaaagata cctatgacga cgacctggat
      841 aacctgctgg cgcagatcgg cgatcagtat gctgacctgt ttctggcggc caaaaacctg
      901 agcgacgcga ttctgctgag cgacatcctg cgtgtgaaca ccgagattac caaagcgccg
      961 ctgagcgcca gcatgattaa gcgttacgac gaacaccacc aggacctgac cctgctgaaa
     1021 gccctggtgc gccagcagct gccggagaag tacaaagaaa tctttttcga tcagagcaaa
     1081 aacggttatg ccggttatat tgacggtggc gcgagccagg aggagtttta taagttcatt
     1141 aagccgattc tggaaaagat ggacggcacc gaggagctgc tggtgaagct gaaccgcgaa
     1201 gacctgctgc gcaaacagcg tacctttgat aacggtagca ttccgcacca gattcacctg
     1261 ggcgaactgc acgcgattct gcgtcgccag gaggacttct atccgttcct gaaggataac
     1321 cgtgagaaaa ttgagaagat tctgaccttc cgtatcccgt actatgtggg cccgctggct
     1381 cgtggtaaca gccgtttcgc ttggatgacc cgtaaaagcg aggagaccat taccccgtgg
     1441 aacttcgagg aagtggtgga caaaggtgcc agcgcgcaga gctttatcga gcgcatgacc
     1501 aacttcgaca aaaacctgcc gaacgaaaaa gtgctgccga aacacagcct gctgtatgaa
     1561 tacttcaccg tgtataacga actgaccaag gtgaagtatg tgaccgaagg tatgcgcaag
     1621 ccggcttttc tgagcggcga gcagaagaag gcgattgtgg acctgctgtt caagaccaac
     1681 cgtaaagtga ccgtgaagca gctgaaggag gactacttca agaaaattga atgctttgac
     1741 agcgtggaga tcagcggcgt ggaggaccgc ttcaacgcga gcctgggcac ctaccacgat
     1801 ctgctgaaga tcattaagga taaagatttt ctggacaacg aggaaaacga agacatcctg
     1861 gaggatattg tgctgaccct gaccctgttt gaggatcgtg aaatgattga agaacgcctg
     1921 aagacctatg ctcacctgtt cgatgataaa gtgatgaaac agctgaagcg tcgtcgttat
     1981 accggttggg gccgtctgag ccgcaaactg atcaacggca ttcgtgacaa acagagcggc
     2041 aagaccatcc tggattttct gaaaagcgat ggctttgcga accgtaactt tatgcagctg
     2101 attcacgatg atagcctgac cttcaaggag gacattcaga aggcgcaggt gagcggccag
     2161 ggtgacagcc tgcacgagca cattgcgaac ctggcgggca gcccggcgat taagaaaggc
     2221 attctgcaaa ccgtgaaagt ggtggatgaa ctggtgaaag tgatgggccg ccacaagccg
     2281 gagaacattg tgatcgagat ggcccgtgag aaccagacca cccagaaagg ccagaagaac
     2341 agccgcgagc gcatgaagcg tattgaagag ggtatcaagg agctgggcag ccagatcctg
     2401 aaggaacacc cggtggaaaa cacccagctg caaaacgaga aactgtatct gtactacctg
     2461 caaaacggcc gtgacatgta tgtggatcag gaactggaca tcaaccgcct gagcgactac
     2521 gacgtggatg cgatcgtgcc gcagagcttc ctgaaagacg acagcattga taacaaggtg
     2581 ctgacccgta gcgacaagaa ccgtggcaaa agcgataacg tgccgagcga ggaggtggtg
     2641 aaaaagatga aaaactattg gcgccagctg ctgaacgcga agctgattac ccagcgcaag
     2701 ttcgacaacc tgaccaaggc tgagcgtggt ggcctgagcg agctggacaa ggctggcttt
     2761 atcaaacgcc agctggtgga gacccgccag atcaccaagc acgtggcgca gatcctggat
     2821 agccgtatga acaccaaata cgatgagaac gataagctga tccgcgaggt gaaagtgatc
     2881 accctgaaga gcaagctggt gagcgatttt cgcaaggact tccagttcta caaggtgcgc
     2941 gaaatcaaca actaccacca cgcgcacgat gcgtatctga acgcggtggt gggcaccgct
     3001 ctgatcaaga aatatccgaa gctggagagc gagttcgtgt acggtgacta taaagtgtac
     3061 gacgtgcgca aaatgattgc caagagcgag caggagattg gtaaagctac cgctaaatat
     3121 ttcttttaca gcaacatcat gaacttcttt aagaccgaga tcaccctggc caacggtgaa
     3181 atccgtccga agaagaaacg taaggtgggt agcagcggta gcccgaaaaa gaagcgcaaa
     3241 gtgggcagcg acgcgctgga cgatttcgat ctggacatgc tgggcagcga tgcgctggat
     3301 gatttcggcg gtggcagctc tgaggtggag ttttcccacg agtactggat gagacatgcc
     3361 ctgaccctgg ccaagagggc acgcgatgag agggaggtgc ctgtgggagc cgtgctggtg
     3421 ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca cgacccaaca
     3481 gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa ctacagactg
     3541 attgacgcca ccctgtacgg cacattcgag ccttgcgtga tgtgcgccgg cgccatgatc
     3601 cactctagga tcggccgcgt ggtgtttggc gtgaggaacg caaaaaccgg cgccgcaggc
     3661 tccctgatgg acgtgctgca ctaccccggc atgaatcacc gcgtcgaaat taccgaggga
     3721 atcctggcag atgaatgtgc cgccctgctg tgctatttct ttcggatgcc tagacaggtg
     3781 ttcaatgctc agaagaaggc ccagagctcc accgacggcg gtagcggcgg tagcggtggt
     3841 agcggcggca gcggtggtag cggtggcagc ggtggtcgca aacgcccgct gatcgaaacc
     3901 aacggcgaaa ccggtgaaat cgtgtgggat aagggtcgcg atttcgcgac cgtgcgcaag
     3961 gtgctgagca tgccgcaggt gaacatcgtg aagaagaccg aagtgcagac cggcggtttt
     4021 agcaaggaaa gcatcctgcc gaagcgcaac agcgataagc tgatcgcgcg taagaaggac
     4081 tgggacccga aaaagtatgg tggcttcgac agcccgaccg tggcgtacag cgtgctggtg
     4141 gtggccaagg tggaaaaggg caaaagcaaa aaactgaaaa gcgtgaagga gctgctgggt
     4201 attaccatta tggaacgtag cagctttgag aagaacccga tcgacttcct ggaggcgaag
     4261 ggttacaagg aagtgaagaa agatctgatc atcaaactgc cgaagtacag cctgtttgag
     4321 ctggaaaacg gccgtaagcg tatgctggcg agcgcgggtg agctgcaaaa aggtaacgag
     4381 ctggcgctgc cgagcaagta cgtgaacttt ctgtacctgg cgagccacta tgaaaaactg
     4441 aaaggcagcc cggaggacaa cgagcagaag cagctgtttg tggagcagca caagcactat
     4501 ctggacgaaa tcatcgaaca gatcagcgag ttcagcaaac gcgtgattct ggccgacgcc
     4561 aacctggaca aagtgctgag cgcctacaac aagcaccgcg ataaaccgat tcgcgaacag
     4621 gccgagaaca tcattcacct gtttaccctg accaacctgg gcgcgccggc ggcgttcaaa
     4681 tactttgata ccaccattga tcgcaaacgc tacaccagca ccaaggaagt gctggatgcg
     4741 accctgatcc accagagcat taccggtctg tacgaaaccc gcatcgatct gagccagctg
     4801 ggcggcgata gcggtggtag caagcgtccg gcggcgacca aaaaggccgg ccaggcgaag
     4861 aagaagaagg actacaagga tgacgatgac aaaggcagcg gcgccaccaa cttcagcctg
     4921 ctgaagcagg ccggtgacgt ggaagaaaac cccggcccga tggcgaagcc gctgagccag
     4981 gaggaaagca ccctgattga gcgcgccacc gcgaccatca acagcattcc gattagcgaa
     5041 gactacagcg tggcgagcgc cgctctgagc agcgatggcc gtatcttcac cggcgtgaac
     5101 gtgtaccact ttaccggcgg tccgtgcgcg gaactggtgg tgctgggcac cgcggcggct
     5161 gcggctgcgg gtaacctgac ctgcatcgtg gccattggta acgaaaaccg cggcattctg
     5221 agcccgtgcg gccgttgccg ccaggtgctg ctggacctgc accccggtat caaagcgatt
     5281 gtgaaagata gcgatggtca accgaccgct gtgggtatcc gtgaactgct gccatccggc
     5341 tatgtttggg agggctaagg tacc
//
