LOCUS       sequence-261073-        4560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO1A2
FEATURES             Location/Qualifiers
     source          1..4560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2685..2784)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2801..2820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2802..2820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2825..2841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2825..2841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2943..2962)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2954..3763
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3021..3040)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3910..4498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4399..4418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggcg agacagagaa gcggatcgag acccaccgga tcagatgcct
      721 gtccaagctg aagatgtttc tgctggccat cacatgtgcc ttcgtgtcta agaccctgag
      781 cggctcctac atgaacagca tgctgacaca gatcgagagg cagtttaata tccccaccag
      841 cctggtgggc tttatcaacg gctccttcga gatcggcaat ctgctgctga tcatcttcgt
      901 gagctatttc ggcacaaagc tgcaccgccc catcatgatc ggaatcggat gcgtggtcat
      961 gggactgggc tgttttctga agagcctgcc tcacttcctg atgaaccagt acgagtatga
     1021 gtctaccgtg tctgtgagcg gcaacctgag ctccaatagc ttcctgtgca tggagaatgg
     1081 cacacagatc ctgcggccca cccaggatcc ttctgagtgt acaaaggagg tgaagagcct
     1141 gatgtgggtt tatgtactcg ttggcaacat cgtgagaggc atgggcgaga cccctatcct
     1201 gccactgggc atcagctaca tcgaggactt tgccaagttc gagaattccc ctctgtatat
     1261 cggactggtg gagaccggag caatcatcgg accactgatc ggactgctgc tggcaagctt
     1321 ttgcgccaac gtgtacgtgg atacaggctt cgtgaatacc gacgatctga tcatcacccc
     1381 aacagacacc agatgggtgg gcgcctggtg gtttggcttc ctgatctgtg ccggcgtgaa
     1441 cgtgctgaca gccatcccct tctttttcct gccaaacaca ctgcccaagg agggcctgga
     1501 gaccaatgcc gatatcatca agaacgagaa tgaggacaag cagaaggagg aggtgaagaa
     1561 ggagaagtac ggcatcacca aggattttct gcccttcatg aagtccctgt cttgcaaccc
     1621 tatctatatg ctgtttatcc tggtgtccgt gatccagttt aacgccttcg tgaatatgat
     1681 ctctttcatg cccaagtacc tggagcagca gtatggcatc tctagctccg acgccatctt
     1741 tctgatgggc atctacaatc tgccccctat ctgcatcggc tatatcatcg gcggcctgat
     1801 catgaagaag ttcaagatca ccgtgaagca ggcagcacac atcggatgct ggctgtccct
     1861 gctggagtac ctgctgtatt ttctgtcttt cctgatgaca tgtgagaact ctagcgtggt
     1921 gggcatcaat acctcctacg agggcatccc tcaggatctg tatgtggaga acgacatctt
     1981 tgccgattgc aacgtggact gcaattgtcc tagcaagatc tgggaccccg tgtgcggcaa
     2041 caatggactg agctacctgt ccgcctgcct ggcaggatgt gagacatcca tcggcaccgg
     2101 catcaacatg gtgttccaga attgctcctg tatccagacc tctggcaatt cctctgccgt
     2161 gctgggcctg tgcgataagg gccctgactg ttccctgatg ctgcagtatt ttctgatcct
     2221 gtccgccatg agctccttca tctactctct ggccgccatc ccaggctata tggtgctgct
     2281 gaggtgcatg aagtctgagg agaagagcct gggcgtgggc ctgcacacat tttgtacccg
     2341 cgtgttcgca ggaatcccag caccaatcta cttcggcgcc ctgatggata gcacatgcct
     2401 gcactggggc accctgaagt gcggagagag cggagcctgt aggatctatg actccaccac
     2461 atttcgctac atctatctgg gactgccagc cgccctgagg ggatctagct tcgtgcccgc
     2521 cctgatcatc ctgatcctgc tgagaaagtg ccacctgcca ggagagaacg catcctctgg
     2581 aacagagctg atcgagacca aggtgaaggg caaggagaat gagtgtaagg atatctacca
     2641 gaagagcaca gtgctgaagg acgatgagct gaagaccaag ctggacccag ctttcttgta
     2701 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2761 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2821 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2881 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2941 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     3001 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     3061 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     3121 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3181 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3241 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3301 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3361 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3421 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3481 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3541 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3601 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3661 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3721 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3781 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3841 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3901 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3961 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4021 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4081 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4141 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4201 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4261 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4321 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4381 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
