LOCUS       sequence-261096-        4890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC26A4
FEATURES             Location/Qualifiers
     source          1..4890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3015..3114)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3131..3150)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3132..3150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3155..3171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3155..3171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3273..3292)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3284..4093
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3351..3370)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4240..4828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4729..4748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggcag caccaggagg cagaagcgag ccacctcagc tgcccgagta
      721 ctcttgcagc tatatggtgt ctaggccagt gtacagcgag ctggccttcc agcagcagca
      781 cgagcggaga ctgcaggaga ggaagaccct gcgcgagagc ctggccaagt gctgttcctg
      841 ttctaggaag cgcgcctttg gcgtgctgaa gacactggtg cctatcctgg agtggctgcc
      901 aaagtatcgc gtgaaggagt ggctgctgtc cgacgtgatc tctggcgtga gcaccggact
      961 ggtggccaca ctgcagggaa tggcatacgc actgctggca gccgtgcctg tgggctacgg
     1021 cctgtattct gccttctttc caatcctgac ctatttcatc tttggcacat ctcggcacat
     1081 cagcgtgggc cccttccctg tggtgagcct gatggtgggc tccgtggtgc tgtctatggc
     1141 ccccgacgag cactttctgg tgagctcctc taacggcacc gtgctgaata ccacaatgat
     1201 cgacaccgcc gcccgggata cagccagagt gctgatcgcc tccgccctga cactgctggt
     1261 gggcatcatc cagctgatct tcggcggcct gcagatcggc tttatcgtga ggtacctggc
     1321 cgatcctctg gtgggaggct tcaccacagc agcagcattt caggtgctgg tgtctcagct
     1381 gaagatcgtg ctgaacgtga gcaccaagaa ctacaatggc gtgctgtcca tcatctatac
     1441 cctggtggag atcttccaga acatcggcga cacaaatctg gccgacttca ccgccggcct
     1501 gctgacaatc gtggtgtgca tggccgtgaa ggagctgaac gaccggttca gacacaagat
     1561 cccagtgccc atccctatcg aagtgatcgt gaccatcatc gccacagcca tctcctacgg
     1621 cgccaatctg gagaagaact ataatgccgg catcgtgaag tctatcccca gaggctttct
     1681 gccacccgag ctgcctccag tgtccctgtt ctctgagatg ctggccgcca gcttttccat
     1741 cgcagtggtg gcatacgcaa tcgccgtgag cgtgggcaag gtgtatgcca ccaagtacga
     1801 ctatacaatc gatggcaacc aggagttcat cgcctttggc atcagcaata tcttctccgg
     1861 cttcttttct tgttttgtgg caaccacagc cctgagcagg accgcagtgc aggagtccac
     1921 cggaggcaag acacaggtgg caggcatcat cagcgccgcc atcgtgatga tcgccatcct
     1981 ggccctgggc aagctgctgg agcctctgca gaagtccgtg ctggcagcag tggtcatcgc
     2041 aaacctgaag ggcatgttca tgcagctgtg cgacatccca aggctgtggc gccagaataa
     2101 gatcgatgcc gtgatctggg tgtttacctg tatcgtgtcc atcatcctgg gactggacct
     2161 gggactgctg gcaggactga tcttcggcct gctgacagtg gtgctgagag tgcagtttcc
     2221 ctcttggaac ggcctgggca gcatcccttc caccgatatc tacaagagca caaagaacta
     2281 taagaatatc gaggagcccc agggcgtgaa gatcctgagg ttcagctccc ctatctttta
     2341 cggcaacgtg gacggcttca agaagtgcat caagtccacc gtgggctttg atgccatcag
     2401 ggtgtataat aagcgcctga aggccctgcg gaagatccag aagctgatca agtctggcca
     2461 gctgcgcgcc accaagaatg gcatcatcag cgacgccgtg tccacaaaca atgccttcga
     2521 gccagacgag gatatcgagg acctggagga gctggatatc cccaccaagg agatcgagat
     2581 ccaggtggat tggaactctg agctgcctgt gaaagtgaat gtgccaaagg tgcccatcca
     2641 ctctctggtg ctggactgcg gcgccatcag cttcctggat gtggtgggcg tgcggtccct
     2701 gagagtgatc gtgaaggagt tccagcgcat cgacgtgaac gtgtactttg cctccctgca
     2761 ggattatgtg atcgagaagc tggagcagtg tggcttcttt gacgataaca tccggaagga
     2821 caccttcttt ctgacagtgc acgatgccat cctgtacctg cagaatcagg tgaagtccca
     2881 ggagggccag ggctctatcc tggagaccat cacactgatc caggactgta aggataccct
     2941 ggagctgatc gagaccgagc tgacagagga ggagctggac gtgcaggatg aggccatgcg
     3001 gacactggcc agcgacccag ctttcttgta caaagttggc attataagaa agcattgctt
     3061 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     3121 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     3181 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     3241 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     3301 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     3361 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     3421 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3481 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3541 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     3601 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3661 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3721 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3781 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3841 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3901 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3961 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     4021 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     4081 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     4141 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     4201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4381 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4861 ggccttttgc tggccttttg ctcacatgtt
//
