LOCUS       sequence-261125-        6797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EFF-1 A full length protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Avinoam O, Fridman K, Valansi C, Abutbul I, Zeev-Ben-Mordehai T, 
            Maurer UE, Sapir A, Danino D, Grunewald K, White JM, Podbilewicz B
  TITLE     Conserved eukaryotic fusogens can fuse viral envelopes to cells.
  JOURNAL   Science. 2011 Apr 29;332(6029):589-92. doi: 10.1126/science.1202333.
            Epub 2011 Mar 24.
  PUBMED    21436398
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1202333"; journalName: "Science"; date: 
            "2011-04-29- 29"; volume: "332"; issue: "6029"; pages: "589-92"
COMMENT     Sequence Label: pCAGGS EFF-1::V5
FEATURES             Location/Qualifiers
     source          1..6797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3752..3793
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3815..3834)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3880..3935
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3934..3953)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4296..4312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4296..4312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4309..4331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4320..4336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4344..4350,4351..4368,4369..4374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4389..4410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4469..4664
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4515..4650
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4670..4804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4707..4726)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4761..4780
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4872..4889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5043..5631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5123..5142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5802..6593,6594..6662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6425..6444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6663..6767)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggsgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcga gctcatcgat
     1741 gcatggtacc actatggaac cgccgtttga gtggtctccc cagtttattc tgcttctcct
     1801 agcagttaca acctacggat ttccactcga ggagaaattc gatgggctct tccgtgcaga
     1861 gccaccacac tgttccaaga caccaattgt ccgtgctcaa acatctcaaa atgcgatgtc
     1921 atcaattgca agaggaatgc aaatgcaatt ctcaattggt ttgcacactg cagtctgctt
     1981 cagattatat gaagacactc aactcgcatc ccaagagatc aatgatgatg aaaatgctgg
     2041 aaatcagaca tcacttcttc atactattcg attggaaaag cttgaacatc accatccgat
     2101 aactcagcgg tacacttttg gaatccctga agttcatgcc agttgtatct gtgagtgtga
     2161 tgccacatct tcaacatgta ccgcagaatc tcatcagttt accgcttgtc cagagtctga
     2221 taagtctgat gaaacatctt catgctaccg cacgttcttc ccaaatcaga caccaatcgg
     2281 gtgcagtgaa gacgacatcc ccaagctttg ctgtgacgtt cgattcaagc catataaaaa
     2341 tatgacattc ctcgcagtca aactagaaca acctacaacg tatgcaacat ttgtctacgc
     2401 agcttatgat tttgtaaatg gatattgggt ggaaaaagat aaaactaaaa tccgttctca
     2461 attagatggt ggaacacagg ataggcatct cgaccaaaaa cgtcgaattt cattggctgt
     2521 aactgccgga ggacgagcat ctcatcaact ggagacagga atgtatttct caagaacaag
     2581 taatggagga gaaacagaag aactgagaat gcaaccattg aatgagatta cagacaataa
     2641 ctttgaccgt cttggatggt acagaatgga cgattctggt cattttcatg tgaataatgg
     2701 agttgtgaaa atggatgata ttcacaaggc taaagtgaaa aattgcaaag agcaaactta
     2761 taaatcaatt ctctctgcaa atcattatat gccaggacat ttcaatttga ctcgtccact
     2821 tgaagttatc aaaccgtgga ttcaatctgc tcgaattttt gatagttctc tcagacaagc
     2881 tgttgtaaca catgccgaag gaactaatct tcaaatttca attcacttgg atgacgaggt
     2941 ggaaagtcaa aaccttgtct tcttccataa tgcatcaaga attcgtgact tcagtggatc
     3001 catcatcgtt gattcaaaat caaatcgatt gttcaacttg acagtatatg aggcttctgg
     3061 aaaaattgat ggatcagtga agatgtcaac tggatttgga tctgatacaa ttcacacatt
     3121 cactgcttat gttagtgatc ttcatgcttc aaaccgttct atgattattc cactgccagc
     3181 catcgttgga caaggagcca gagcaatctg tctccgagca gattcaatgg ctgatattga
     3241 caaaatttgt catgtcattg agtatttcga gagccctttg gaaatcgatc ttgttgaggg
     3301 aaaatggcac gaaatgattg gaacctgccc aacttgtaat caaatcaatt tcaatggaat
     3361 gatgaaattt ctgaatccag ctcattggat caaaggaatc agttcgattg gtgacggcgt
     3421 tatgattgct acggatattg ttgtgtatct cggagttttg tgtattctgt atcttcttat
     3481 tacgaagatt attgttccgt tggttcgttg ctgggtatgc cccatgtcga ttttctgcaa
     3541 tgggtcctct tcgtcgtcca aaaacaaaaa tgataagaga aggaaggaga gagaggagag
     3601 gcgcagaaag gacaaatttg tctcagaatc cgaagatggt gcaagaagta gttccgagcc
     3661 ccacgacact ttggcgagat accatggaaa tcactcggag cggcactata gcagtagcca
     3721 gtacattggt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     3781 tctcgattct acgcgtaccg gttgagctag cagatctttt tccctctgcc aaaaattatg
     3841 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     3901 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     3961 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     4021 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     4081 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     4141 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     4201 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     4261 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4321 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4381 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4441 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     4501 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4561 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4621 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     4681 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4741 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4801 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4861 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4921 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4981 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5461 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6781 aaagtgccac ctgggtc
//
