LOCUS       sequence-261126-        6919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express AtHAP2 full length protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Valansi C, Moi D, Leikina E, Matveev E, Grana M, Chernomordik LV, 
            Romero H, Aguilar PS, Podbilewicz B
  TITLE     Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to 
            somatic and viral fusogens.
  JOURNAL   J Cell Biol. 2017 Mar 6;216(3):571-581. doi: 10.1083/jcb.201610093. 
            Epub 2017 Jan 30.
  PUBMED    28137780
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201610093"; journalName: "J Cell Biol"; date: 
            "2017-03-6- 6"; volume: "216"; issue: "3"; pages: "571-581"
COMMENT     Sequence Label: pCAGGS::AtHAP2-V5
FEATURES             Location/Qualifiers
     source          1..6919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3872..3913
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3941..3960)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4006..4061
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4060..4079)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4422..4438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4422..4438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4435..4457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4446..4462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4470..4476,4477..4494,4495..4500))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4515..4536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4595..4790
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4641..4776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4796..4930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4833..4852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4887..4906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4998..5015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5169..5757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5249..5268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5928..6719,6720..6788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6551..6570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6789..6893)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgagctcat
     1741 cgatatggtg aacgcgattt taatggcttg tatactcgcc ggaattttcg tcggaatgtt
     1801 caacgaagtc gacggaattc agattttatc gaaatcaaag ctcgaaaagt gtgagaaaac
     1861 atcagattct ggtaatctca attgttccac taagatcgtc ttgaatctcg ctgttcccag
     1921 tggatccagt ggaggagagg cttcgattgt agcggagatc gtggaggttg aagataactc
     1981 gagtagtaat atgcaaaccg tacggattcc accggttatt acagtgaaca aatctgcagc
     2041 atatgctctc tatgatctta cttacattag ggatgtacct tataaacctc aagagtatca
     2101 tgtcacgact cgaaagtgtg aacccgatgc tggaccggat attgtacaaa tttgtgagag
     2161 gttgagagat gagaagggta atgttttaga gcagactcag ccgatttgtt gtccatgtgg
     2221 accgcaacga agaatgcctt cttcttgtgg agatattttt gataagatga ttaaagggaa
     2281 ggcaaatacc gcgcattgcc ttcgttttcc aggtgactgg ttccatgttt tcgggattgg
     2341 acaacggtca ctaggattta gtgtgcgagt tgagttgaaa acaggaacta gagtttcaga
     2401 agtgatcatt ggtcctgaaa ataggacagc tactgcaaat gataactttc tcaaagttaa
     2461 tctcatagga gactttggtg gttacacaag tatcccttct ttcgaggact tctatcttgt
     2521 aatcccaaga gaggcggctg aagcgggaca accagggagc ttaggcgcaa attactcaat
     2581 gtggatgctg cttgagagag tgagatttac attggatggc ttagaatgta ataaaatcgg
     2641 tgttggttat gaagctttca acacccaacc aaatttctgc tcttctccat attggagttg
     2701 cttgcataat caactatgga acttccgtga gtcagatata aacagaatcg accgacacca
     2761 acttccttta tatggacttg aaggaagatt tgagaggatc aatcaacacc caaacgcggg
     2821 gcctcactcg ttctcaattg gagtcacgga aacactcaac acaaatctaa tgatagaact
     2881 aagagctgat gatattgaat acgttttcca gaggagtccc ggtaagatca taaacatcgc
     2941 gataccgaca ttcgaagcac ttacacaatt cggagttgct gcagtcataa tcaagaatac
     3001 tggtgaagta gaagcgtctt atagcttaac gtttgattgc tccaaaggcg tggcttttgt
     3061 tgaggaacaa tttttcatta taaaaccgaa agcggttact actcggtctt ttaagctgta
     3121 cccgacgaaa gatcaggctg caaagtatat atgcacagct atactcaagg actctcagtt
     3181 cagcgaagta gaccgagccg agtgtcaatt tagtacaacg gctaccgtac ttgacaatgg
     3241 aactcaggtt actaatccat ttcaaattcc agagactcaa cctaaaggtt tctttgattc
     3301 gatcaggata ctatggatga aaatcatcaa tggtttagtc gatttcatca ccggagatac
     3361 ttgcagaaac aaatgctcga gtttcttcga cttcagttgc cacattcaat acgtttgctt
     3421 aagctggatg gttatgtttg gtcttctcct cgctctcttc ccaattactt gtttgcttct
     3481 ttggctcttg caccaaaagg gactctttga tccatgctac gactggtggg aagatcattt
     3541 cgatttagac catcatcgga gattattacc ctccagagca gacgttgtta accgccatca
     3601 tcaccatcac aagcatcgtc atcaccataa tcaccatcgt aggacacacc agagacacaa
     3661 acatcatcat ggtcaagacg acgacgtatt gcagaagatg atgcttgaac gtgaccacag
     3721 cgactctcac tactaccacc aacttcatcg agtccataaa gatagcaagc agaagcagcg
     3781 taggcgagcc aagcacggga tcgtcctgcc acgtgatgtg cacgtggaaa gacagaggaa
     3841 acaaagacta cgtgagagtc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     3901 tctcgattct acgtgacccg ggcatgctcg agctagcaga tctttttccc tctgccaaaa
     3961 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     4021 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     4081 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     4141 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     4201 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     4261 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     4321 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     4381 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4441 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4501 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4561 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     4621 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4681 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4741 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     4801 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4861 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4921 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4981 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5041 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5101 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5161 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5221 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5281 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5341 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5401 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5461 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5521 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5581 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5641 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5701 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5761 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5821 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5881 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5941 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6001 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6061 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6121 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6181 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6241 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6301 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6361 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6421 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6481 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6541 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6601 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6661 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6721 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6781 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6841 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6901 ccccgaaaag tgccacctg
//
