LOCUS       sequence-261129-        8243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an spCas9 under the control of a CMV promoter; The spCas9 
            is flanked by COL4A3 sgRNA targets to induce its self-cleaving and 
            inactivation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Daga S, Donati F, Capitani K, Croci S, Tita R, Giliberti A, 
            Valentino F, Benetti E, Fallerini C, Niccheri F, Baldassarri M, 
            Mencarelli MA, Frullanti E, Furini S, Conticello SG, Renieri A, 
            Pinto AM
  TITLE     New frontiers to cure Alport syndrome: COL4A3 and COL4A5 gene 
            editing in podocyte-lineage cells.
  JOURNAL   Eur J Hum Genet. 2019 Nov 21. pii: 10.1038/s41431-019-0537-8. doi: 
            10.1038/s41431-019-0537-8.
  PUBMED    31754267
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J Hum 
            Genet. 2019 Nov 21. pii: 10.1038/s41431-019-0537-8. doi: 
            10.1038/s41431-019-0537-8."
COMMENT     Sequence Label: pAAV_COL4A3_self-cleavingCas9
FEATURES             Location/Qualifiers
     source          1..8243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     promoter        621..824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     775..795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             889..954
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             961..981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1006..5106
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPPIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5107..5154
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5250..5267)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5256..5463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5551..5680
                     /label=AAV2 ITR
     primer_bind     complement(5699..5716)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5699..5715)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5708..5730)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5857..6312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5944..5963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6154..6175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6338..6442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6443..6511,6512..7303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6661..6680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7474..8062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7963..7982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagtcg acggtatcga
      481 taagcttgat tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatccgcta gagcttggat cccctggaga aactgaatca ccaggatgga ctataaggac
      901 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
      961 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
     1021 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1081 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1141 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     1201 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     1261 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     1321 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     1381 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     1441 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     1501 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     1561 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     1621 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     1681 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     1741 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     1801 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     1861 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     1921 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     1981 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2041 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2101 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2161 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     2221 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     2281 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     2341 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     2401 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     2461 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     2521 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     2581 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     2641 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     2701 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     2761 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     2821 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     2881 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     2941 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3001 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3061 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3121 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3181 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     3241 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     3301 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     3361 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     3421 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     3481 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     3541 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     3601 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     3661 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     3721 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     3781 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     3841 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     3901 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     3961 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4021 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4081 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4141 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctccgat cgagacaaac
     4201 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     4261 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     4321 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     4381 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     4441 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     4501 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     4561 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     4621 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     4681 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     4741 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     4801 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     4861 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     4921 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     4981 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     5041 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5101 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagtaagaa
     5161 ttcctagagc tcgctgacct ggtgattcag tttctccagg ccgcggcctg ctgccggctc
     5221 tgcggcctct tccgcgtctt cgagatctgc ctcgactgtg ccttctagtt gccagccatc
     5281 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5341 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5401 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5461 ggactcgagt taagggcgaa ttcccgataa ggatcttcct agagcatggc tacgtagata
     5521 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc
     5581 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     5641 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac
     5701 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5761 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5821 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     5881 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5941 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6001 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6061 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6121 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6181 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6241 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6301 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6361 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6421 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6481 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6541 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6601 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6661 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6721 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6781 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6841 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6901 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6961 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7021 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7081 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7141 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7201 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7261 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7321 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7381 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7441 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7501 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7561 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7621 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7681 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7741 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7801 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7861 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7921 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7981 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8041 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8101 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8161 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8221 gtcagtgagc gaggaagcgg aag
//
