LOCUS       sequence-261130-        7560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express AtHAP2 full length protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Valansi C, Moi D, Leikina E, Matveev E, Grana M, Chernomordik LV, 
            Romero H, Aguilar PS, Podbilewicz B
  TITLE     Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to 
            somatic and viral fusogens.
  JOURNAL   J Cell Biol. 2017 Mar 6;216(3):571-581. doi: 10.1083/jcb.201610093. 
            Epub 2017 Jan 30.
  PUBMED    28137780
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201610093"; journalName: "J Cell Biol"; date: 
            "2017-03-6- 6"; volume: "216"; issue: "3"; pages: "571-581"
COMMENT     Sequence Label: pCAGGS::AtHAP2-YFP
FEATURES             Location/Qualifiers
     source          1..7560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(3866..3868,3869..3871,3872..4384)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(3911..3932)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4172..4191)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4519..4540
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4582..4601)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4647..4702
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4701..4720)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5063..5079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5063..5079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5076..5098)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5087..5103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5111..5117,5118..5135,5136..5141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5156..5177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5236..5431
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5282..5417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5437..5571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5474..5493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5528..5547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5639..5656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5810..6398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5890..5909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6569..7360,7361..7429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7192..7211
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7430..7534)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgagctcat
     1741 cgatatggtg aacgcgattt taatggcttg tatactcgcc ggaattttcg tcggaatgtt
     1801 caacgaagtc gacggaattc agattttatc gaaatcaaag ctcgaaaagt gtgagaaaac
     1861 atcagattct ggtaatctca attgttccac taagatcgtc ttgaatctcg ctgttcccag
     1921 tggatccagt ggaggagagg cttcgattgt agcggagatc gtggaggttg aagataactc
     1981 gagtagtaat atgcaaaccg tacggattcc accggttatt acagtgaaca aatctgcagc
     2041 atatgctctc tatgatctta cttacattag ggatgtacct tataaacctc aagagtatca
     2101 tgtcacgact cgaaagtgtg aacccgatgc tggaccggat attgtacaaa tttgtgagag
     2161 gttgagagat gagaagggta atgttttaga gcagactcag ccgatttgtt gtccatgtgg
     2221 accgcaacga agaatgcctt cttcttgtgg agatattttt gataagatga ttaaagggaa
     2281 ggcaaatacc gcgcattgcc ttcgttttcc aggtgactgg ttccatgttt tcgggattgg
     2341 acaacggtca ctaggattta gtgtgcgagt tgagttgaaa acaggaacta gagtttcaga
     2401 agtgatcatt ggtcctgaaa ataggacagc tactgcaaat gataactttc tcaaagttaa
     2461 tctcatagga gactttggtg gttacacaag tatcccttct ttcgaggact tctatcttgt
     2521 aatcccaaga gaggcggctg aagcgggaca accagggagc ttaggcgcaa attactcaat
     2581 gtggatgctg cttgagagag tgagatttac attggatggc ttagaatgta ataaaatcgg
     2641 tgttggttat gaagctttca acacccaacc aaatttctgc tcttctccat attggagttg
     2701 cttgcataat caactatgga acttccgtga gtcagatata aacagaatcg accgacacca
     2761 acttccttta tatggacttg aaggaagatt tgagaggatc aatcaacacc caaacgcggg
     2821 gcctcactcg ttctcaattg gagtcacgga aacactcaac acaaatctaa tgatagaact
     2881 aagagctgat gatattgaat acgttttcca gaggagtccc ggtaagatca taaacatcgc
     2941 gataccgaca ttcgaagcac ttacacaatt cggagttgct gcagtcataa tcaagaatac
     3001 tggtgaagta gaagcgtctt atagcttaac gtttgattgc tccaaaggcg tggcttttgt
     3061 tgaggaacaa tttttcatta taaaaccgaa agcggttact actcggtctt ttaagctgta
     3121 cccgacgaaa gatcaggctg caaagtatat atgcacagct atactcaagg actctcagtt
     3181 cagcgaagta gaccgagccg agtgtcaatt tagtacaacg gctaccgtac ttgacaatgg
     3241 aactcaggtt actaatccat ttcaaattcc agagactcaa cctaaaggtt tctttgattc
     3301 gatcaggata ctatggatga aaatcatcaa tggtttagtc gatttcatca ccggagatac
     3361 ttgcagaaac aaatgctcga gtttcttcga cttcagttgc cacattcaat acgtttgctt
     3421 aagctggatg gttatgtttg gtcttctcct cgctctcttc ccaattactt gtttgcttct
     3481 ttggctcttg caccaaaagg gactctttga tccatgctac gactggtggg aagatcattt
     3541 cgatttagac catcatcgga gattattacc ctccagagca gacgttgtta accgccatca
     3601 tcaccatcac aagcatcgtc atcaccataa tcaccatcgt aggacacacc agagacacaa
     3661 acatcatcat ggtcaagacg acgacgtatt gcagaagatg atgcttgaac gtgaccacag
     3721 cgactctcac tactaccacc aacttcatcg agtccataaa gatagcaagc agaagcagcg
     3781 taggcgagcc aagcacggga tcgtcctgcc acgtgatgtg cacgtggaaa gacagaggaa
     3841 acaaagacta cgtgagagta gagccatggt gagcaagggc gaggagctgt tcaccggggt
     3901 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3961 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     4021 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     4081 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     4141 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     4201 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     4261 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4321 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     4381 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4441 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     4501 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4561 cggcatgctc gagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     4621 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     4681 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     4741 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     4801 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     4861 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     4921 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     4981 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     5041 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5101 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5161 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5221 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5281 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5341 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5401 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     5461 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5521 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     5581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6241 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6481 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6541 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6601 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6661 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6721 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6781 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6841 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6901 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6961 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7021 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7081 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7141 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7201 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7261 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7321 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7381 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7441 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7501 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
