LOCUS       sequence-261144-        4623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO1B1
FEATURES             Location/Qualifiers
     source          1..4623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2748..2847)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2864..2883)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2865..2883)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2888..2904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2888..2904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3006..3025)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3017..3826
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3084..3103)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3973..4561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4462..4481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggacc agaaccagca cctgaataag accgccgagg cccagccctc
      721 cgagaacaag aagacacggt actgcaatgg cctgaagatg tttctggccg ccctgtccct
      781 gtctttcatc gccaagaccc tgggcgccat catcatgaag agctccatca tccacatcga
      841 gcggagattt gagatctcta gctccctggt gggctttatc gatggcagct tcgagatcgg
      901 caacctgctg gtcatcgtgt tcgtgagcta cttcggctcc aagctgcaca ggccaaagct
      961 gatcggcatc ggctgtttca tcatgggaat cggaggcgtg ctgaccgccc tgccccactt
     1021 ctttatgggc tactatcgct attccaagga gaccaacatc aattctagcg agaatagcac
     1081 ctccacactg agcacatgcc tgatcaacca gatcctgtct ctgaataggg caagccctga
     1141 gatcgtgggc aagggatgtc tgaaggagtc tggcagctac atgtggatct acgtgttcat
     1201 gggcaacatg ctgagaggca tcggagagac cccaatcgtg cctctgggac tgagctacat
     1261 cgacgatttc gccaaggagg gccactcctc tctgtatctg ggcatcctga atgccatcgc
     1321 catgatcggc ccaatcatcg gctttaccct gggctctctg ttcagcaaga tgtacgtgga
     1381 catcggctat gtggatctgt ccacaatcag gatcacccca acagactcta gatgggtggg
     1441 agcatggtgg ctgaactttc tggtgtctgg cctgttcagc atcatcagct ccatcccttt
     1501 ctttttcctg ccacagaccc ccaacaagcc tcagaaggag aggaaggcct ccctgtctct
     1561 gcacgtgctg gagacaaatg acgagaagga tcagaccgcc aacctgacaa atcagggcaa
     1621 gaacatcacc aagaatgtga caggcttttt ccagtccttt aagtctatcc tgaccaatcc
     1681 tctgtacgtg atgttcgtgc tgctgacact gctgcaggtg tctagctata tcggcgcctt
     1741 tacctacgtg ttcaagtatg tggagcagca gtacggccag ccctcctcta aggccaacat
     1801 cctgctgggc gtgatcacaa tccctatctt tgcctccggc atgttcctgg gcggctatat
     1861 catcaagaag tttaagctga ataccgtggg catcgccaag tttagctgct tcacagccgt
     1921 gatgagcctg tccttctacc tgctgtactt cttcatcctg tgcgagaaca agtccgtggc
     1981 cggcctgacc atgacatacg acggcaacaa tccagtgacc agccacaggg atgtgcccct
     2041 gtcctattgc aactctgact gcaattgtga tgagtcccag tgggagcccg tgtgcggcaa
     2101 caatggcatc acatacatct ctccatgcct ggccggctgt aagagctcct ctggcaacaa
     2161 gaagccaatc gtgttttaca attgctcttg tctggaggtg accggcctgc agaaccgcaa
     2221 ttatagcgcc cacctgggag agtgcccaag ggacgatgca tgtacaagaa agttctactt
     2281 cttcgtggcc atccaggtgc tgaacctgtt tttcagcgcc ctgggaggca cctcccacgt
     2341 gatgctgatc gtgaagatcg tgcagcctga gctgaagtct ctggccctgg gctttcacag
     2401 catggtcatc cgggccctgg gaggaatcct ggcaccaatc tacttcggcg ccctgatcga
     2461 caccacatgc atcaagtgga gcaccaacaa ttgcggcaca aggggctcct gtcgcaccta
     2521 caactctaca agcttctccc gggtgtatct gggcctgagc tccatgctga gagtgtctag
     2581 cctggtgctg tacatcatcc tgatctatgc catgaagaag aagtaccagg agaaggacat
     2641 caacgccagc gagaatggct ccgtgatgga tgaggccaat ctggagagcc tgaacaagaa
     2701 taagcacttc gtgccatccg ccggagcaga ttctgagacc cactgcgacc cagctttctt
     2761 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2821 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2881 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2941 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     3001 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3061 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3121 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3181 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3241 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3301 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3361 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3421 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3481 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3541 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3601 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3661 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3721 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3781 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3841 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3901 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4561 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4621 gtt
//
