LOCUS       sequence-261149-        5223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC4A11
FEATURES             Location/Qualifiers
     source          1..5223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3348..3447)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3464..3483)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3465..3483)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3488..3504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3488..3504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3606..3625)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3617..4426
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3684..3703)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4573..5161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5062..5081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgagcc aagtgggagg aaggggcgat aggtgcacac aggaggtgca
      721 gggactggtg cacggagcag gcgacctgtc cgcctctctg gccgagaact cccccaccat
      781 gtctcagaat ggctatttcg aggatagctc ctactataag tgtgacaccg acgatacatt
      841 tgaggcaagg gaggagatcc tgggcgacga ggccttcgat accgccaact ctagcatcgt
      901 gagcggcgag tccatccgct tctttgtgaa cgtgaatctg gagatgcagg ccacaaacac
      961 cgaaaatgaa gcaaccagcg gcggatgcgt gctgctgcac acctctcgca agtacctgaa
     1021 gctgaagaac ttcaaggagg agatccgggc ccacagagac ctggatggct tcctggccca
     1081 ggccagcatc gtgctgaacg agacagccac ctccctggat aatgtgctga ggaccatgct
     1141 gcggagattt gcccgcgacc ctgataacaa tgagccaaac tgtaatctgg atctgctgat
     1201 ggcaatgctg ttcacagacg caggagcacc aatgaggggc aaggtgcacc tgctgtccga
     1261 caccatccag ggagtgacag caaccgtgac aggcgtgcgg taccagcagt cttggctgtg
     1321 catcatctgt accatgaagg ccctgcagaa gagacacgtg tgcatctcca ggctggtgcg
     1381 ccctcagaac tggggagaga attcttgtga ggtgcggttc gtgatcctgg tgctggcacc
     1441 acctaagatg aagtctacca agacagccat ggaggtggcc agaaccttcg ccacaatgtt
     1501 tagcgatatc gcctttcggc agaagctgct ggagaccaga acagaggagg agttcaagga
     1561 ggccctggtg caccagaggc agctgctgac catggtgtcc cacggacctg tggcaccaag
     1621 gaccaaggag agatctacag tgagcctgcc agcacacagg caccctgagc cacccaagtg
     1681 caaggatttc gtgccttttg gcaagggcat cagggaggac atcgcaaggc gcttcccact
     1741 gtatcctctg gactttacag atggcatcat cggcaagaac aaggccgtgg gcaagtatat
     1801 caccacaacc ctgttcctgt actttgcctg tctgctgcca accatcgcct ttggcagcct
     1861 gaacgacgag aatacagatg gcgccatcga cgtgcagaag accatcgcag gacagtctat
     1921 cggaggactg ctgtatgccc tgttcagcgg ccagcccctg gtcatcctgc tgacaaccgc
     1981 ccctctggcc ctgtacatcc aggtcatcag agtgatctgc gacgattatg acctggattt
     2041 caactccttt tacgcctgga caggcctgtg gaattctttc tttctggccc tgtacgcctt
     2101 ctttaatctg agcctggtca tgtccctgtt taagagaagc accgaggaga tcatcgccct
     2161 gttcatctcc atcacatttg tgctggatgc cgtgaagggc accgtgaaga tcttctggaa
     2221 gtactattac ggccactatc tggacgatta ccacaccaag aggacatcct ctctggtgag
     2281 cctgtccgga ctgggagcat ctctgaacgc aagcctgcac accgccctga atgcctcctt
     2341 tctggcctct cctaccgagc tgccaagcgc cacacactcc ggacaggcaa ccgccgtgct
     2401 gtccctgctg atcatgctgg gcacactgtg gctgggctat accctgtacc agttcaagaa
     2461 gagcccctat ctgcaccctt gcgtgaggga gatcctgtcc gactgtgcac tgccaatcgc
     2521 cgtgctggcc ttctctctga tcagctccca cggctttcgg gagatcgaga tgagcaagtt
     2581 cagatacaac ccatctgaga gcccctttgc catggcccag atccagtccc tgtctctgag
     2641 ggccgtgtct ggagcaatgg gactgggctt cctgctgagc atgctgttct ttatcgagca
     2701 gaacctggtg gccgccctgg tgaacgcacc agagaatcgc ctggtgaagg gcacagccta
     2761 ccactgggat ctgctgctgc tggccatcat caataccgga ctgagcctgt tcggactgcc
     2821 atggatccac gcagcatatc cacactcccc actgcacgtg agggccctgg ccctggtgga
     2881 ggagagggtg gagaacggcc acatctacga caccatcgtg aatgtgaagg agaccagact
     2941 gacatctctg ggagcaagcg tgctggtggg actgtccctg ctgctgctgc cagtgcctct
     3001 gcagtggatc ccaaagcccg tgctgtatgg cctgtttctg tacatcgccc tgacaagcct
     3061 ggacggaaac cagctggtgc agagggtggc cctgctgctg aaggagcaga ccgcctatcc
     3121 tccaacacac tacatccgga gagtgccaca gagaaagatc cactatttca ccggactgca
     3181 ggtgctgcag ctgctgctgc tgtgcgcctt tggcatgtct agcctgccat acatgaagat
     3241 gatcttcccc ctgatcatga tcgccatgat ccctatcagg tatatcctgc tgccacgcat
     3301 catcgaggcc aagtacctgg acgtgatgga tgccgagcac aggcctgacc cagctttctt
     3361 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     3421 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     3481 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3541 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     3601 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3661 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3721 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3781 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3841 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3901 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3961 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     4021 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     4081 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     4141 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     4201 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     4261 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     4321 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     4381 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     4441 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     4501 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     4561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4681 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4801 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4921 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4981 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5041 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5101 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5161 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5221 gtt
//
