LOCUS       sequence-261165-        4758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC6A16
FEATURES             Location/Qualifiers
     source          1..4758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2883..2982)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2999..3018)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3000..3018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3023..3039)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3023..3039)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3141..3160)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3152..3961
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3219..3238)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4108..4696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4597..4616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaaga ccgaggccca gccttccaca tctctgctgg ccaacaccag
      721 ctggaccggc acagtgatca gcgactccgt gccaggctct cagacatggg aggataaggg
      781 cagcctgacc aggtctgcca ccagctggac atccgaggcc caggtgtccg ccgcaagggt
      841 ggcagaggca caggcacgca catctcagcc caagcagatc agcgtgctgg aggccctgac
      901 cgcatccgcc ctgaaccaga agcctacaca cgagaaggtg cagatgaccg agaagaagga
      961 gtctgaggtg ctgctggccc ggccattctg gagctccaag accgagtaca tcctggccca
     1021 agtgggcttc tccatgaagc cctcttgcct gtggagattt gcctacctgt ggctgaatag
     1081 cggcggctgt tcctttgccg ccatctatat cttcatgctg tttctggtgg gcgtgccact
     1141 gctgttcctg gagatggcag caggacagag catgaggcag ggaggaatgg gcgtgtggaa
     1201 gatcatcgcc ccttggatcg gcggcgtggg ctattctagc ttcatggtgt gctttattct
     1261 gggcttgtac ttcaatgtag taaattcctg gatcatcttt tatatgtctc agagcttcca
     1321 gtttccagtg ccctgggaga agtgccccct gaccatgaac tcctctggct tcgaccccga
     1381 gtgtgagagg accacacctt ccatctactt ttggtatcag caggccctga aggcctctga
     1441 ccgcatcgag gatggcggct ctccagtgta cagcctggtg ctgcccttct ttctgtgctg
     1501 gtgtctggtg ggcgccttta tgatcaatgg cctgaagagc acaggcaaag tgatctacgt
     1561 gctggtgctg ctgccctgtt ttatcatcgt gggcttcttt atccggaccc tgctgctgga
     1621 gggagcaaag ttcggactgc agcagctggt ggtggccaag atctctgacg tgtacaacat
     1681 gagcgtgtgg agcctggcag gaggacaggt gctgagcaat accggaatcg gactgggatc
     1741 cgtggcatct ctggccagct atatgcctca gagcaacaat tgcctgtccg atgccttcct
     1801 ggtgagcgtg atcaacctgc tgaccctgct ggtgttcaca tccttcaatt tttgcgtgct
     1861 gggcttttgg gccaccgtga tcacacaccg gtgctgtgag agaaacgccg agatcctgct
     1921 gaagctgatc aatctgggca agctgccacc tgacgcaaag ccacccgtga acctgctgta
     1981 caatcccacc agcatctata acgcctggct gtccggcctg cctcagcaca tcaagagcat
     2041 ggtgctgcgg gaggtgacag agtgcaatat cgagacccag ttcctgaagg cctccgaggg
     2101 ccctaagttc gcctttctgt ccttcgtgga ggccatgtct tttctgcctc catccgtgtt
     2161 ctggtctttt atcttctttc tgatgctgct ggcaatggga ctgagctccg ccatcggcat
     2221 catgcagggc atcatcaccc cactgcagga taccttcagc ttcttcagaa agcacacaaa
     2281 gctgctgatc gtgggcgtgt tcctgctgat gttcgtgtgc ggcctgttct ttacccggcc
     2341 atccggctct tacttcatca gactgctgtc tgactattgg atcgtgttcc ccatcatcgt
     2401 ggtggtggtg tttgagacaa tggccgtgtc ctgggcatac ggagcacgga gattcctggc
     2461 agatctgacc atcctgctgg gccaccccat ctcccctatc tttggctggc tgtggcctca
     2521 cctgtgccca gtggtgctgc tgatcatctt cgtgacaatg atggtgcacc tgtgcatgaa
     2581 gcctatcaca tacatgagct gggattctag cacctccaag gaggtgctga ggccatatcc
     2641 cccttgggcc ctgctgctga tgatcaccct gttcgccatc gtgatcctgc ctatcccagc
     2701 ctacttcgtg tattgcagga tccaccgcat cccatttcgc ccaaagagcg gcgacggacc
     2761 aatgaccgca agcacatccc tgcctctgtc tcaccagctg acaccaagca aggaggtgca
     2821 gaaggaggag atcctgcagg tggatgagac caagtacccc tccacctgta atgtgacatc
     2881 tgacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2941 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     3001 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     3061 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     3121 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     3181 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     3241 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     3301 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     3361 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     3421 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     3481 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     3541 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     3601 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     3661 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     3721 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     3781 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     3841 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3901 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3961 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     4021 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     4081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4741 gccttttgct cacatgtt
//
