LOCUS       sequence-261167-       10299 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative control for ATP measurement, Tol2 transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Yaginuma H, Kawai S, Tabata KV, Tomiyama K, Kakizuka A, Komatsuzaki 
            T, Noji H, Imamura H
  TITLE     Diversity in ATP concentrations in a single bacterial cell 
            population revealed by quantitative single-cell imaging.
  JOURNAL   Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038/srep06522.
  PUBMED    25283467
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-10-6"; volume: "4:6522. doi"; pages: " 
            10.1038/srep06522"
COMMENT     Sequence Label: pT2MP.EF-QNA
FEATURES             Location/Qualifiers
     source          1..10299
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    22..70
                     /note="synthetic polyadenylation signal"
     promoter        105..1287
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          336..1278
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1235..1255
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1333..1342
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1699..1950
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1887..1908
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2014..2035)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2275..2294)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2541..2765
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2812..2835)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3209..3228
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3293..3892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3293..3312)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3789..3809
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3979..4203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4231..4247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4255..4271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4279..4285,4286..4303,4304..4309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4324..4345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(5661..5798,5799..5810)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(5829..5847)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5868..5884)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5892..5908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5916..5922,5923..5940,5941..5946))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5961..5982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6099..6116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6270..6858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6350..6369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7029..7820,7821..7889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7652..7671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7890..7994)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8020..8475)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8157..8178)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8369..8388
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8602..8624
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8616..8633
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8617..8633
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8640..8659
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8640..8658
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(8672..8683,8684..8871)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    10140..10188
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cagtgaggat ggggctctag gaataaaata tctttatttt cattacatct gtgtgttggt
       61 tttttgtgtg gtcgacattg attattgact agatcatcgc gtgaggctcc ggtgcccgtc
      121 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
      181 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
      241 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
      301 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
      361 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg
      421 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
      481 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
      541 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
      601 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
      661 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
      721 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
      781 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
      841 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
      901 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
      961 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     1021 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     1081 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     1141 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     1201 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     1261 ttttttcttc catttcaggt gtcgtgagga attctgcagt cgacggtacc gcgggcccgg
     1321 gatccaccgg tcgccaccat ggtgaaaact gtgaaagtga atataacaac ccctgatggg
     1381 ccagtctacg acgctgatat cgagatggtg tccgtgcggg ccgagagtgg tgatctcggc
     1441 atcctccccg gtcacattcc cacaaaggcc ccactgaaga tcggagctgt gcggctgaag
     1501 aaggacggcc aaaccgagat ggtcgcagtc tcaggcggca ctgttgaagt gcggcctgac
     1561 cacgttacca ttaatgctca agccgctgaa acagccgaag gaatcgacaa agagagagca
     1621 gaagccgcaa gacagagggc ccaggagcgg ctgaactctc aaacccgtta caacagccac
     1681 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1741 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     1861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1921 atcactctcg gcatggacga gctgtacaag ggtggcaccg gagggagcat ggtgagcaag
     1981 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2041 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2101 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2161 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2221 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     2281 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2341 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2401 aacctgggtg acaccgatat tcgccgggcc gagctggcac tgcagaaggc cctgaacaag
     2461 ctggacgtgg ctgggaaggc aaactgagcg gccgctcgat aagcttgatc gatgatctag
     2521 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     2581 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     2641 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     2701 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     2761 tatggcttct gaggcggaaa gaacctgcag cttttcccaa ggcagtctgg agcatgcgct
     2821 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     2881 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     2941 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     3001 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     3061 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     3121 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     3181 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     3241 gcgctgttct cctcttcctc atctccgggc ctttcgacct gtggccacaa ccatgaccga
     3301 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     3361 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     3421 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     3481 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     3541 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     3601 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     3661 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     3721 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     3781 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     3841 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     3901 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tgatctagag
     3961 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4021 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4081 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4141 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4201 tggctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4261 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4321 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4381 aaacctgtcg tgccagcgga tccctagagg gacagccccc ccccaaagcc cccagggatg
     4441 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
     4501 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
     4561 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
     4621 ggggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag
     4681 aacggcctgg gttgcaaagg agcacagtgc tcatccagat ccaaccccct gctatgtgca
     4741 gggtcatcaa ccagcagccc aggctgccca gagccacatc cagcctggcc ttgaatgcct
     4801 gcagggatgg ggcatccaca gcctccttgg gcaacctgtt cagtgcgtca ccaccctctg
     4861 ggggaaaaac tgcctcctca tatccaaccc aaacctcccc tgtctcagtg taaagccatt
     4921 cccccttgtc ctatcaaggg ggagtttgct gtgacattgt tggtctgggg tgacacatgt
     4981 ttgccaattc agtgcatcac ggagaggcag atcttgggga taaggaagtg caggacagca
     5041 tggacgtggg acatgcaggt gttgagggct ctgggacact ctccaagtca cagcgttcag
     5101 aacagcctta aggataagaa gataggatag aaggacaaag agcaagttaa aacccagcat
     5161 ggagaggagc acaaaaaggc cacagacact gctggtccct gtgtctgagc ctgcatgttt
     5221 gatggtgtct ggatgcaagc agaaggggtg gaagagcttg cctggagaga tacagctggg
     5281 tcagtaggac tgggacaggc agctggagaa ttgccatgta gatgttcata caatcgtcaa
     5341 atcatgaagg ctggaaaagc cctccaagat ccccaagacc aaccccaacc cacccaccgt
     5401 gcccactggc catgtccctc agtgccacat ccccacagtt cttcatcacc tccagggacg
     5461 gtgacccccc cacctccgtg ggcagctgtg ccactgcagc accgctcttt ggagaaggta
     5521 aatcttgcta aatccagccc gaccctcccc tggcacaacg taaggccatt atctctcatc
     5581 caactccagg acggagtcag tgaggatggg gctctagccg gaccgacgcg tctcgatagg
     5641 gataacaggg taattgatcc aatactcaag tacaatttta atggagtact tttttacttt
     5701 tactcaagta agattctagc cagatacttt tacttttaat tgagtaaaat tttccctaag
     5761 tacttgtact ttcacttgag taaaattttt gagtactttt tacacctctg gagctccagc
     5821 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     5881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5941 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6001 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6061 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6241 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7141 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7801 ttcaccagcg tttctgggtg agcaaaaacg ggaaggcaaa atgccgcaaa aaagggaata
     7861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7981 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     8041 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     8101 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     8161 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     8221 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     8281 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     8341 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     8401 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     8461 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     8521 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     8581 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     8641 aatacgactc actatagggc gaattgggta ccagaggtgt aaagtacttg agtaatttta
     8701 cttgattact gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa
     8761 aaatcaatac ttttactttt acttaattac atttttttag aaaaaaaagt actttttact
     8821 ccttacaatt ttatttacag tcaaaaagta cttatttttt ggagatcact tgtcgagtag
     8881 ggataacagg gtaatgatcc cccgggctgc agtcgcgacg cgtcctagag ggacagcccc
     8941 cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc
     9001 gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg
     9061 gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc
     9121 tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga
     9181 tttagaatga cagaatcata gaacggcctg ggttgcaaag gagcacagtg ctcatccaga
     9241 tccaaccccc tgctatgtgc agggtcatca accagcagcc caggctgccc agagccacat
     9301 ccagcctggc cttgaatgcc tgcagggatg gggcatccac agcctccttg ggcaacctgt
     9361 tcagtgcgtc accaccctct gggggaaaaa ctgcctcctc atatccaacc caaacctccc
     9421 ctgtctcagt gtaaagccat tcccccttgt cctatcaagg gggagtttgc tgtgacattg
     9481 ttggtctggg gtgacacatg tttgccaatt cagtgcatca cggagaggca gatcttgggg
     9541 ataaggaagt gcaggacagc atggacgtgg gacatgcagg tgttgagggc tctgggacac
     9601 tctccaagtc acagcgttca gaacagcctt aaggataaga agataggata gaaggacaaa
     9661 gagcaagtta aaacccagca tggagaggag cacaaaaagg ccacagacac tgctggtccc
     9721 tgtgtctgag cctgcatgtt tgatggtgtc tggatgcaag cagaaggggt ggaagagctt
     9781 gcctggagag atacagctgg gtcagtagga ctgggacagg cagctggaga attgccatgt
     9841 agatgttcat acaatcgtca aatcatgaag gctggaaaag ccctccaaga tccccaagac
     9901 caaccccaac ccacccaccg tgcccactgg ccatgtccct cagtgccaca tccccacagt
     9961 tcttcatcac ctccagggac ggtgaccccc ccacctccgt gggcagctgt gccactgcag
    10021 caccgctctt tggagaaggt aaatcttgct aaatccagcc cgaccctccc ctggcacaac
    10081 gtaaggccat tatctctcat ccaactccag gacggagtca gtgaggatgg ggctctagga
    10141 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtggt cgacattgat
    10201 tattgactag atcatcgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
    10261 cacagtcccc gagaagttgg ggggaggggt cggcaattg
//
