LOCUS       sequence-261169-        8179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Rimbault C, Maruthi K, Breillat C, Genuer C, Crespillo S, 
            Puente-Munoz V, Chamma I, Gauthereau I, Antoine S, Thibaut C, Tai 
            FWJ, Dartigues B, Grillo-Bosch D, Claverol S, Poujol C, Choquet D, 
            Mackereth CD, Sainlos M
  TITLE     Engineering selective competitors for the discrimination of highly 
            conserved protein-protein interaction modules.
  JOURNAL   Nat Commun. 2019 Oct 4;10(1):4521. doi: 10.1038/s41467-019-12528-4.
  PUBMED    31586061
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-4"; volume: "10(1):4521. doi"; pages: " 
            10.1038/s41467-019-12528-4"
COMMENT     Sequence Label: pCAG-mIRFP670nuc-TEV-Xph20-ETWV
FEATURES             Location/Qualifiers
     source          1..8179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..36
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     enhancer        40..419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          697..1713
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1637..1659
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1721..1740
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1785..1794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1791..2735
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             2748..2768
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2772..2792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2796..2816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2859..3563
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLM"
     CDS             3591..3611
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     intron          4071..4203
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4186..4205
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4212..4800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4265..4285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4683..4694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4827..4844)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4833..4944
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5040..5060)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5040..5058)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5079..5095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5201..5220)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5266..5321
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5320..5339)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5682..5698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5706..5722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5730..5736,5737..5754,5755..5760))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5775..5796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5855..6050
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5901..6036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6056..6190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6093..6112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6147..6166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6258..6275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6429..7017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6509..6528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7188..7979,7980..8048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7811..7830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8049..8153)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagagtcatc ctcatcgtcg acattgatta ttgactagtt
       61 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      421 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      481 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      541 ggcgcgccrg gsggggsggg ggggggsggg gggsggggsg gggsgrggcg gagaggkgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      721 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      781 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      841 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
      901 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      961 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1021 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1081 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1141 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     1201 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     1261 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggrgggc
     1321 tcgggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1381 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1441 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     1501 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1561 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1621 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1681 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1741 ctgtctcatc attttggcaa agaattcggt accgcgggcc cggggccacc atggtagcag
     1801 gtcatgcctc tggcagcccc gcattcggga ccgcctctca ttcgaattgc gaacatgaag
     1861 agatccacct cgccggctcg atccagccgc atggcgcgct tctggtcgtc agcgaacatg
     1921 atcatcgcgt catccaggcc agcgccaacg ccgcggaatt tctgaatctc ggaagcgtac
     1981 tcggcgttcc gctcgccgag atcgacggcg atctgttgat caagatcctg ccgcatctcg
     2041 atcccaccgc cgaaggcatg ccggtcgcgg tgcgctgccg gatcggcaat ccctctacgg
     2101 agtactgcgg tctgatgcat cggcctccgg aaggcgggct gatcatcgaa ctcgaacgtg
     2161 ccggcccgtc gatcgatctg tcaggcacgc tggcgccggc gctggagcgg attcgcacgg
     2221 cgggttcact gcgcgcgctg tgcgatgaca ccgtgctgct gtttcagcag tgcaccggct
     2281 acgaccgggt gatggtgtat cgtttcgatg agcaaggcca cggcctggta ttctccgagt
     2341 gccatgtgcc tgggctcgaa tcctatttcg gcaaccgcta tccgtcgtcg actgtcccgc
     2401 agatggcgcg gcagctgtac gtgcggcagc gcgtccgcgt gctggtcgac gtcacctatc
     2461 agccggtgcc gctggagccg cggctgtcgc cgctgaccgg gcgcgatctc gacatgtcgg
     2521 gctgcttcct gcgctcgatg tcgccgtgcc atctgcagtt cctgaaggac atgggcgtgc
     2581 gcgccaccct ggcggtgtcg ctggtggtcg gcggcaagct gtggggcctg gttgtctgtc
     2641 accattatct gccgcgcttc atccgtttcg agctgcgggc gatctgcaaa cggctcgccg
     2701 aaaggatcgc gacgcggatc accgcgcttg agagcagatc tgctgatcca aaaaagaaga
     2761 gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtaggat
     2821 cgaccggatc tagaaagctt caattgggaa gcggaagcgg agaaagcttg tttaaggggc
     2881 cgcgtgatta caacccgata tcgagcacca tttgtcattt gacgaatgaa tctgatgggc
     2941 acacaacatc gttgtatggt attggatttg gtcccttcat cattacaaac aagcacttgt
     3001 ttagaagaaa taatggaaca ctgttggtcc aatcactaca tggtgtattc aaggtcaaga
     3061 acaccacgac tttgcaacaa cacctcattg atgggaggga catgataatt attcgcatgc
     3121 ctaaggattt cccaccattt cctcaaaagc tgaaatttag agagccacaa agggaagagc
     3181 gcatatgtct tgtgacaacc aacttccaaa ctaagagcat gtctagcatg gtgtcagaca
     3241 ctagttgcac attcccttca tctgatggca tattctggaa gcattggatt caaaccaagg
     3301 atgggcagtg tggcagtcca ttagtatcaa ctagagatgg gttcattgtt ggtatacact
     3361 cagcatcgaa tttcaccaac acaaacaatt atttcacaag cgtgccgaaa aacttcatgg
     3421 aattgttgac aaatcaggag gcgcagcagt gggttagtgg ttggcgatta aatgctgact
     3481 cagtattgtg ggggggccat aaagttttca tggtgaaacc tgaagagcct tttcagccag
     3541 ttaaggaagc gactcaactc atgggttctt ccggctctag cggctctagt gagaatttgt
     3601 attttcaggg cggatccgct agctccgtgc cgaccaaact ggaagtggtt gcggccaccc
     3661 cgacgtctct gctgatcagc tgggatgcag tggcgaagaa tgtgaaggtc ggttattacc
     3721 gtatcaccta tggcgaaacg ggcggtaact ccccggttca ggaattcacc gtcccgggta
     3781 gttcctcaac cgcaacgatt tccggcctga aaccgggtgt cgactatacc attacggttt
     3841 acgctaatgg ggtgctgagt aagatggtgc ttccgatttc tatcaattac cgtaccctcg
     3901 agggcgggag tggagggtcc ggtggcagtg gcggttcggg tgggtcaggc ggttcgggcg
     3961 gttctggcgg ctctggcggt agcggaggca gcggtggttc cggcggaagc ggtaccgaaa
     4021 cctgggtgta ataatgtaca agtaaagcgg ccccggactc gaggccgcag gtaagtatca
     4081 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     4141 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     4201 caggtgtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4261 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4321 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4381 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4441 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4501 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4561 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4621 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4681 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4741 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4801 ctggaattcg agctcggtac gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4861 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4921 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4981 tggggtgggg caggacagca agggggagga ttgggaagac aatagcccag cttttgttcc
     5041 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     5101 aattgttatc cgctaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     5161 tgtggccaat gccctggctc acaaatacca ctgagatcga tctttttccc tctgccaaaa
     5221 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     5281 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     5341 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     5401 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     5461 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     5521 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     5581 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     5641 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5701 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5761 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5821 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     5881 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6001 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     6061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6181 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6241 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6421 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6601 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6841 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6901 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6961 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7321 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8161 ccccgaaaag tgccacctg
//
