LOCUS       sequence-261173-        4656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO1B3
FEATURES             Location/Qualifiers
     source          1..4656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2781..2880)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2897..2916)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2898..2916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2921..2937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2921..2937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3039..3058)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3050..3859
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3117..3136)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4006..4594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4495..4514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggacc agcaccagca cctgaacaag acagccgagt ccgccagctc
      721 cgagaagaag aagacccgga gatgcaatgg ctttaagatg ttcctggccg ccctgtcctt
      781 ctcttacatc gccaaggccc tgggcggcat catcatgaag atctccatca cccagatcga
      841 gaggcgcttc gacatctcta gctccctggc cggcctgatc gatggcagct ttgagatcgg
      901 caacctgctg gtcatcgtgt tcgtgagcta cttcggcagc aagctgcacc ggcctaagct
      961 gatcggcatc ggctgtctgc tgatgggcac aggctccatc ctgacctctc tgcctcactt
     1021 ctttatgggc tactatagat atagcaagga gacacacatc aacccatctg agaatagcac
     1081 atctagcctg tccacctgcc tgatcaacca gacactgtcc ttcaatggca cctctccaga
     1141 gatcgtggag aaggactgcg tgaaggagag cggctcccac atgtggatct acgtgttcat
     1201 gggcaacatg ctgaggggaa tcggagagac cccaatcgtg cctctgggca tctcttacat
     1261 cgacgatttt gccaaggagg gccactcctc tctgtatctg ggctccctga atgccatcgg
     1321 catgatcgga cccgtgatcg gattcgcact gggaagcctg tttgccaaga tgtacgtgga
     1381 catcggctat gtggatctgt ccacaatcag gatcacccca aaggattcta gatgggtggg
     1441 agcatggtgg ctgggcttcc tggtgagcgg cctgttttcc atcatcagct ccatcccctt
     1501 ctttttcctg ccaaagaacc ccaataagcc tcagaaggag aggaagatct ctctgagcct
     1561 gcacgtgctg aagacaaacg acgatcgcaa tcagacagcc aacctgacca atcagggcaa
     1621 gaacgtgaca aagaatgtga ccggcttttt ccagtccctg aagtctatcc tgacaaaccc
     1681 actgtacgtg atcttcctgc tgctgaccct gctgcaggtg tctagcttta tcggcagctt
     1741 cacctacgtg tttaagtata tggagcagca gtatggccag tccgcctctc acgccaattt
     1801 cctgctgggc atcatcacca tccccacagt ggccaccggc atgttcctgg gcggctttat
     1861 catcaagaag tttaagctgt ctctggtggg catcgccaag tttagcttcc tgacaagcat
     1921 gatctccttt ctgttccagc tgctgtactt ccctctgatc tgcgagagca agtccgtggc
     1981 aggactgacc ctgacatacg acggcaacaa ttctgtggcc agccacgtgg atgtgccact
     2041 gtcttattgc aacagcgagt gcaattgtga cgagtcccag tgggagcccg tgtgcggaaa
     2101 caatggcatc acctatctgt ctccctgcct ggccggctgt aagtcctcta gcggcatcaa
     2161 gaagcacaca gtgttttaca actgctcttg cgtggaggtg accggcctgc agaaccggaa
     2221 ttatagcgcc cacctgggag agtgccctag ggataacacc tgtacaagaa agtttttcat
     2281 ctacgtggcc atccaggtca tcaactccct gttctctgcc acaggcggca ccaccttcat
     2341 cctgctgacc gtgaagatcg tgcagccaga gctgaaggcc ctggcaatgg gcttccagtc
     2401 catggtcatc aggaccctgg gaggaatcct ggcacctatc tactttggcg ccctgatcga
     2461 taagacatgc atgaagtgga gcaccaactc ctgcggagca cagggagcct gtcggatcta
     2521 caatagcgtg tttttcggca gagtgtatct gggactgtcc atcgccctga ggttccccgc
     2581 cctggtgctg tatatcgtgt ttatcttcgc catgaagaag aagtttcagg gcaaggacac
     2641 caaggccagc gataacgagc gcaaagtgat ggacgaggcc aatctggagt tcctgaacaa
     2701 tggcgagcac tttgtgccta gcgccggcac agattccaag acctgtaacc tggacatgca
     2761 ggataacgcc gccgccaatg acccagcttt cttgtacaaa gttggcatta taagaaagca
     2821 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2881 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2941 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     3001 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     3061 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     3121 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     3181 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3241 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3301 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     3361 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3421 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3481 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3541 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3601 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3661 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3721 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3781 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3841 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     3901 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     3961 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4021 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4081 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4141 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4201 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4261 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4321 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4381 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4441 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4501 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4561 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4621 gttcctggcc ttttgctggc cttttgctca catgtt
//
