LOCUS       sequence-261178-        8191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-BRD2
FEATURES             Location/Qualifiers
     source          1..8191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(709..711,712..714,715..1425)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(754..775)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1015..1034)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1362..1383
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1441..1465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3947..3971)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3979..4020
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4060..4079)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4104..4152
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(4355..4371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4355..4371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4368..4390)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4379..4395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4403..4409,4410..4427,4428..4433))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4448..4469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4586..4603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4757..5345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4837..4856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5516..6307,6308..6376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6139..6158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6377..6481)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(6893..8071)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6902..7840
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6925..6945)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatccctacc ggtgatatcc tcgagaccat ggtgagcaag
      721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      781 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      901 ttcacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      961 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1141 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg
     1201 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1261 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1321 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1381 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggctc gagcccatca
     1441 acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa ccaattctct aaggaaatac
     1501 ttaaccatgg tcgactggat ccggatgctg caaaacgtga ctccccacaa taagctccct
     1561 ggggaaggga atgcagggtt gctggggctg ggcccagaag cagcagcacc agggaagagg
     1621 attcgaaaac cctctctctt gtatgagggc tttgagagcc ccacaatggc ttcggtgcct
     1681 gctttgcaac ttacccctgc caacccacca cccccggagg tgtccaatcc caaaaagcca
     1741 ggacgagtta ccaaccagct gcaataccta cacaaggtag tgatgaaggc tctgtggaaa
     1801 catcagttcg catggccatt ccggcagcct gtggatgctg tcaaactggg tctaccggat
     1861 tatcacaaaa ttataaaaca gcctatggac atgggtacta ttaagaggag acttgaaaac
     1921 aattattatt gggctgcttc agagtgtatg caagatttta ataccatgtt caccaactgt
     1981 tacatttaca acaagcccac tgatgatatt gtcctaatgg cacaaacgct ggaaaagata
     2041 ttcctacaga aggttgcatc aatgccacaa gaagaacaag agctggtagt gaccatccct
     2101 aagaacagcc acaagaaggg ggccaagttg gcagcgctcc agggcagtgt taccagtgcc
     2161 catcaggtgc ctgccgtctc ttctgtgtca cacacagccc tgtatactcc tccacctgag
     2221 atacctacca ctgtcctcaa cattccccac ccatcagtca tttcctctcc acttctcaag
     2281 tccttgcact ctgctggacc cccgctcctt gctgttactg cagctcctcc agcccagccc
     2341 cttgccaaga aaaaaggcgt aaagcggaaa gcagatacta ccacccctac acctacagcc
     2401 atcttggctc ctggttctcc agctagccct cctgggagtc ttgagcctaa ggcagcacgg
     2461 cttcccccta tgcgtagaga gagtggtcgc cccatcaagc ccccacgcaa agacttgcct
     2521 gactctcagc aacaacacca gagctctaag aaaggaaagc tttcagaaca gttaaaacat
     2581 tgcaacggca ttttgaagga gttactctct aagaagcatg ctgcctatgc ttggcctttc
     2641 tataaaccag tggatgcttc tgcacttggc ctgcatgact accatgacat cattaagcac
     2701 cccatggacc tcagcactgt caagcggaag atggagaacc gtgattaccg ggatgcacag
     2761 gagtttgctg ctgatgtacg gcttatgttc tccaactgct ataagtacaa tcccccagat
     2821 cacgatgttg tggcaatggc acgaaagcta caggatgtat ttgagttccg ttatgccaag
     2881 atgccagatg aaccactaga accagggcct ttaccagtct ctactgccat gccccctggc
     2941 ttggccaaat cgtcttcaga gtcctccagt gaggaaagta gcagtgagag ctcctctgag
     3001 gaagaggagg aggaagatga ggaggacgag gaggaagaag agagtgaaag ctcagactca
     3061 gaggaagaaa gggctcatcg cttagcagaa ctacaggaac agcttcgggc agtacatgaa
     3121 caactggctg ctctgtccca gggtccaata tccaagccca agaggaaaag agagaaaaaa
     3181 gagaaaaaga agaaacggaa ggcagagaag catcgaggcc gagctggggc cgatgaagat
     3241 gacaaggggc ctagggcacc ccgcccacct caacctaaga agtccaagaa agcaagtggc
     3301 agtgggggtg gcagtgctgc tttaggccct tctggctttg gaccttctgg aggaagtggc
     3361 accaagctcc ccaaaaaggc cacaaagaca gccccacctg ccctgcctac aggttatgat
     3421 tcagaggagg aggaagagag caggcccatg agttacgatg agaagcggca gctgagcctg
     3481 gacatcaaca aattacctgg ggagaagctg ggccgagttg tgcatataat ccaagccagg
     3541 gagccctctt tacgtgattc aaacccagaa gagattgaga ttgattttga aacactcaag
     3601 ccatccacac ttagagagct tgagcgctat gtcctttcct gcctacgtaa gaaaccccgg
     3661 aagccctaca ccattaagaa gcctgtggga aagacaaagg aggaactggc tttggagaaa
     3721 aagcgggaat tagaaaagcg gttacaagat gtcagcggac agctcaattc tactaaaaag
     3781 ccccccaaga aagcgaatga gaaaacagag tcatcctctg cacagcaagt agcagtgtca
     3841 cgccttagcg cttccagctc cagctcagat tccagctcct cctcttcctc gtcgtcgtct
     3901 tcagacacca gtgattcaga ctcaggctta cactcgagat atctagaccc agctttcttg
     3961 tacaaagtgg ttgataacgg gaagcctatc cctaaccctc tcctcggtct cgattctacg
     4021 cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga aggagacaat
     4081 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     4141 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     4201 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     4261 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     4321 atctgcgcag ctggggaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4381 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4441 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4501 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4561 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4621 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4681 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4741 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4801 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4861 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4921 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4981 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5041 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5101 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5161 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5221 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5281 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5341 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5401 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5461 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5521 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5581 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5641 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5701 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5761 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5821 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5881 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5941 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6001 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6061 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6121 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6181 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6241 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6301 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6361 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6421 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6481 gcacatttcc ccgaaaagtg catctgcaga attcggcttc ccggtcaaca tccagtacac
     6541 ctccggcacc accggcttcc ccaagggcgc cacgctctcc caccacaaca tcctcaacaa
     6601 cggctactgg gtgggccgca cggtcggcta cacggagcag gaccgggtgt gtctgccggt
     6661 gcccttctac cactgcttcg gcatggtcat gggcaacttg ggcgccacct cccacgccgc
     6721 ctgcatcgtc atcccggccc cgtccttcga gccggcggcg acgctggagg cggtgcagcg
     6781 ggagaggtgc acgtccctgt acggcgtccc cacgaagccg aattccacca cactggacta
     6841 gatgcatgct cgagcggccg ccagtgtgat ggatatctgc agaattcggc tttcacgaca
     6901 cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc
     6961 caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt
     7021 cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta
     7081 aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact
     7141 ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg
     7201 actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcaggac
     7261 cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc
     7321 ggggcggggc gatacacggc ggcgcgagga caggcaccag agcaggccgg ccagcttgag
     7381 actacccccg tccgattctc ggtggtcgcg ctcgcaggcc ccgcctcgcc gaacatgtgc
     7441 gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca
     7501 gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc
     7561 aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc
     7621 agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag
     7681 gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat
     7741 caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg
     7801 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc
     7861 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct
     7921 cgggaaaaag gcggagccag tacacggcat cactttccca gtttaccccg cgccaccttc
     7981 tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg
     8041 tgcgctctgc ccactgacgg gcaccggagc caagccgaat tccagcacac tggcggccgt
     8101 tactagtgga tccgagctcg gtacctgaag aatctgctta gggttaggcg ttttgcgctg
     8161 cttcgcgatg tacgggccag atatacgcgt t
//
