LOCUS       sequence-261184-        6117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human WDR60, codon optimised for Sf9 expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, 
            Roberts AJ
  TITLE     Structure of the dynein-2 complex and its assembly with 
            intraflagellar transport trains.
  JOURNAL   Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. 
            doi: 10.1038/s41594-019-0286-y.
  PUBMED    31451806
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 
            10.1038/s41594-019-0286-y."
COMMENT     Sequence Label: WDR60
FEATURES             Location/Qualifiers
     source          1..6117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      148..736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     637..656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  871..1094
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1161..1694)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1883..1888,1889..1905,1906..1911))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1982..2015)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2130..2220
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2145..2162
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    5618..5739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5655..5674)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5709..5728
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5910..6075
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
       61 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct
      841 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
      901 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
      961 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1021 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     1081 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     1141 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     1201 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     1261 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     1321 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     1381 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     1441 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     1501 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     1561 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     1621 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     1681 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1741 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1801 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1861 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1921 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc
     1981 cataacttcg tatagcatac attatacgaa gttatctgta actataacgg tcctaaggta
     2041 gcgacgttta aacatccacc atttggaaca attctattga acttggctcg gtcgcgtcga
     2101 cgatcatggc tctcgaaatg taatgcctgt catggagata attaaaatga taaccatctc
     2161 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     2221 attccggatt attcataccg tcccaccatc gggcgcggat cctcaacatg gaacctggta
     2281 agagaaggac taaagatgac acctggaaag cagatgattt gaggaaacac ctgtgggcta
     2341 tccagtccgg aggcagcaag gaggaacgca agcaccgcga gaagaagctg agaaaggaat
     2401 ctgagatgga tttgcctgaa cataaagagc ccagatgtcg cgatccagat caagacgcca
     2461 ggagcaggga tagagttgcc gaggtccaca cagctaagga atcaccacgc ggtgagaggg
     2521 accgcgacag acaaagggag agacgcagag atgcaaaaga tagagagaag gagaagctga
     2581 aggagaagca tagagaggca gagaagtctc actctagggg caaagaccgc gagaaagaga
     2641 aagacagaag agccagaaaa gaggagctga gacagactgt cgcacatcac aatttgttgg
     2701 gacaggaaac aagagatcgc caactgctgg aacgcgcaga gcgcaaagga cgctcagtgt
     2761 ctaaggtgag gagcgaagag aaggacgaag actctgaacg cggagacgaa gacagggaaa
     2821 ggcgctatag ggaaaggaag ctgcaatacg gcgactcaaa ggataatccc ctgaaatact
     2881 ggttgtataa ggaggaaggt gaaagaaggc accgcaagcc tcgcgaaccc gacagagaca
     2941 agaagcatag agaaaagtca agcaccagag agaaaagaga gaagtactcc aaagagaaat
     3001 caaacagctt ttccgacaag ggagaagagc gccacaagga aaagaggcac aaggagggat
     3061 ttcatttcga cgacgaacgc caccagtcaa acgtggatcg caaagagaag agcgctaaag
     3121 acgagcctcg caaacgcgag tttcagaacg gcgagcacag gaacagagga gcctccagca
     3181 agcgcgatgg cacctctagc cagcacgctg aaaacctcgt gaggaaccac ggcaaggaca
     3241 aagattctcg caggaaacac ggacacgaag agggaagctc agtctggtgg aagctggacc
     3301 aaagacctgg cggtgaggag actgtggaga tcgagaagga agagactgac ctggaaaacg
     3361 ctagagccga cgcatacacc gcttcttgtg aggatgactt tgaggactac gaggacgatt
     3421 tcgaggtctg cgacggcgat gacgatgagt catccaatga acccgagagc agagagaagt
     3481 tggaggaact ccctctcgcc cagaagaagg agattcaaga gattcagaga gcaatcaatg
     3541 ctgagaacga gagaattggc gaactctcac tgaaactgtt tcagaagagg ggaagaactg
     3601 agttcgagaa ggaacctagg actgacacaa actcatcccc ttctcgcgct tctgtttgtg
     3661 gcattttcgt tgatttcgca tccgcatctc acaggcagaa gtcaagaact caagctctca
     3721 agcaaaagat gcgctccact aaactgctca gactgatcga cctggacttt tcctttacat
     3781 tcagcctgct cgacctccca cctgtcaacg aatacgatat gtacatcaga aactttggca
     3841 agaagaatac taaacaggcc tatgtgcagt gcaatgagga taatgtggaa agagacattc
     3901 agaccgaaga gattgaaaca cgcgaagttt ggacccagca tccaggcgaa agcactgttg
     3961 tctctggagg ctccgagcag agagacacaa gcgacgccgt ggtgatgccc aagattgaca
     4021 cccctaggtt gtgcagcttc ctcagagcag cctgccaggt catggccgtg ctgctggagg
     4081 aagataggct cgctgccgag ccttcttgga acctgagagc ccaagacaga gcactgtatt
     4141 tctcagacag ctcttcacag ttgaacacca gcctgccatt cttgcaaaac cgcaaagtct
     4201 cctccctgca tacctctagg gtgcagcgcc agatggtggt gtccgtgcac gacttgcctg
     4261 aaaagtcctt cgtgcccctg ctggactcca agtatgtgtt gtgcgtgtgg gacatctggc
     4321 aaccctccgg accccagaaa gtgctgattt gtgaatcaca agtgacctgc tgttgtctct
     4381 ccccactgaa ggcctttctg ctctttgccg gtactgcaca cggaagcgtg gtcgtgtggg
     4441 acctgagaga ggactctcgc ctccactatt ccgtcacact gtctgacgga ttctggacct
     4501 ttagaactgc aacattttcc acagatggca ttctgacttc agtgaatcac aggagcccct
     4561 tgcaggccgt ggagccaatc agcacctcag tgcacaagaa gcaatcattt gttctgagcc
     4621 ccttctctac tcaggaagag atgtcaggcc tctcttttca catcgcaagc ctggatgagt
     4681 ctggtgtcct caacgtttgg gtcgtggtcg aactcccaaa agctgacatc gctggttcaa
     4741 tctctgatct cggtttgatg cctggtggta gagtcaagtt ggtgcacagc gctctgatcc
     4801 agctgggtga tagcctgagc cataaaggca atgagttctg gggtactacc cagactttga
     4861 acgtcaaatt cctgcccagc gaccctaacc acttcatcat cggcaccgat atgggcctga
     4921 tttctcacgg tactaggcag gacctgagag ttgcccctaa actgttcaag ccccagcaac
     4981 acggaatcag accagttaag gtgaacgtga ttgacttctc tcccttcggc gagcccatct
     5041 tcctcgctgg ctgtagcgac ggttcaatca gattgcacca gctgtcttct gctttcccac
     5101 tgctccagtg ggactcttcc accgattcac atgctgtgac aggtttgcag tggtcaccta
     5161 ctcgccctgc tgtgttcctg gttcaagacg atacttccaa catctacatc tgggacctgc
     5221 tccaatctga tctcggacca gttgccaagc agcaggtgtc tccaaaccgc ctcgtcgcaa
     5281 tggctgccgt gggcgaacct gagaaggctg gcggttcttt cctggctctg gttctcgctc
     5341 gcgcttccgg ctctattgac attcagcact tgaaacgcag gtgggctgcc ccagaagtgg
     5401 atgagtgcaa taggctgagg ctgttgctgc aagaagcctt gtggcctgag ggtaagctgc
     5461 acaagtagct agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat
     5521 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     5581 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     5641 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5701 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga aacatccacc
     5761 atttaaatgg agttaggttt aaacggatct gatcactgct tgagcctaga agatccggct
     5821 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactatca
     5881 taacccctag ggtataccca tctaattgga accagataag tgaaatctag ttccaaacta
     5941 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt
     6001 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta
     6061 ttttgtccgc ccacaaccgg ttgacttggg tcaactgtca gaccaagttt actcata
//
