LOCUS       sequence-261198-        6192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC4A7
FEATURES             Location/Qualifiers
     source          1..6192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4317..4416)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4433..4452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4434..4452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4457..4473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4457..4473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4575..4594)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4586..5395
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4653..4672)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5542..6130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6031..6050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggaga ggttccgcct ggagaagaag ctgcctggcc cagatgagga
      721 ggccgtggtg gacctgggca agaccagctc cacagtgaat accaagtttg agaaggagga
      781 gctggagagc cacagggccg tgtacatcgg agtgcacgtg cccttctcca aggagtctcg
      841 gagaaggcac cggcacagag gccacaagca ccaccaccgc cggagaaagg acaaggagtc
      901 tgataaggag gacggccggg agtctcctag ctatgatacc ccaagccaga gagtgcagtt
      961 tatcctgggc acagaggacg atgacgagga gcacatccct cacgatctgt tcaccgagat
     1021 ggacgagctg tgctaccggg atggcgagga gtatgagtgg aaggagacag ccagatggct
     1081 gaagtttgag gaggatgtgg aggacggagg cgataggtgg agcaagccat acgtggccac
     1141 cctgagcctg cactccctgt tcgagctgag gagctgtatc ctgaatggca cagtgatgct
     1201 ggatatgagg gcatccaccc tggacgagat cgcagatatg gtgctggaca acatgatcgc
     1261 ctctggccag ctggacgaga gcatcaggga gaatgtgcgc gaggccctgc tgaagaggca
     1321 ccaccaccag aacgagaagc ggtttacctc cagaatcccc ctggtgagat ctttcgccga
     1381 catcggcaag aagcactccg atcctcacct gctggagagg aatggagagg gactgtccgc
     1441 ctctaggcac agcctgagga caggactgag cgcctccaac ctgtccctga gaggcgagtc
     1501 tccactgagc ctgctgctgg gacacctgct gccatctagc agggcaggaa ccccagcagg
     1561 atctaggtgc accacacccg tgcctacacc acagaacagc cccccttcct ctccctctat
     1621 cagccggctg acctccagaa gctcccagga gtctcagagg caggcaccag agctgctggt
     1681 gtcccctgcc tctgatgaca tcccaacagt ggtcatccac ccaccagagg aggacctgga
     1741 ggccgccctg aagggcgagg agcagaagaa cgaggagaat gtggatctga ccccaggaat
     1801 cctggcaagc ccacagtccg cccctggcaa cctggacaat tccaagtctg gcgagatcaa
     1861 gggcaatggc tctggcggca gcagggagaa ctccaccgtg gatttctcta aggtggacat
     1921 gaacttcatg cgcaagatcc ctacaggagc agaggcaagc aacgtgctgg tgggagaggt
     1981 ggacttcctg gagaggccca tcatcgcctt tgtgcgcctg gcacctgccg tgctgctgac
     2041 aggactgacc gaggtgccag tgcctaccag attcctgttt ctgctgctgg gaccagcagg
     2101 caaggcacct cagtaccacg agatcggcag gtccatcgcc acactgatga ccgatgagat
     2161 ctttcacgac gtggcctata aggccaagga cagaaatgat ctgctgtccg gcatcgatga
     2221 gttcctggac caggtgaccg tgctgcctcc aggcgagtgg gatccttcta tccggatcga
     2281 gccccctaag tccgtgcctt ctcaggagaa gagaaagatc ccagtgttcc acaacggaag
     2341 cacccccaca ctgggagaga cccctaagga ggcagcacac cacgcaggac cagagctgca
     2401 gaggacaggc cgcctgtttg gcggcctgat cctggacatc aagcgcaagg cccccttctt
     2461 tctgagcgac ttcaaggatg ccctgagcct gcagtgcctg gcctccatcc tgtttctgta
     2521 ctgcgcctgt atgtccccag tgatcacatt cggcggactg ctgggagagg caaccgaggg
     2581 aaggatcagc gccatcgagt ccctgtttgg agcaagcctg accggaatcg catactccct
     2641 gttcgcagga cagccactga caatcctggg atccaccgga cccgtgctgg tgttcgagaa
     2701 gatcctgtat aagttttgcc gggattacca gctgagctat ctgtccctga gaacatctat
     2761 cggcctgtgg accagctttc tgtgcatcgt gctggtggcc accgacgcct ctagcctggt
     2821 gtgctacatc acaaggttca ccgaggaggc ctttgccgcc ctgatctgta tcatcttcat
     2881 ctacgaggcc ctggagaagc tgttcgacct gggcgagaca tatgccttta atatgcacaa
     2941 caatctggat aagctgacaa gctattcctg cgtgtgcacc gagccaccca acccaagcaa
     3001 tgagacactg gcccagtgga agaaggataa catcaccgcc cacaatatct cttggaggaa
     3061 cctgacagtg agcgagtgca agaagctgag gggcgtgttt ctgggatccg cctgtggaca
     3121 ccacggacca tacatccctg acgtgctgtt ctggtgcgtg atcctgttct ttaccacatt
     3181 ctttctgtcc tctttcctga agcagtttaa gaccaagcgg tacttcccca caaaggtgag
     3241 atctaccatc agcgacttcg ccgtgtttct gacaatcgtg atcatggtga ccatcgatta
     3301 tctggtgggc gtgccatctc ccaagctgca cgtgcccgag aagtttgagc caacccaccc
     3361 agagagggga tggatcatct ccccactggg cgataatccc tggtggacac tgctgatcgc
     3421 cgccatccct gccctgctgt gcaccatcct gatcttcatg gaccagcaga tcacagccgt
     3481 gatcatcaac cggaaggagc acaagctgaa gaagggcgcc ggctatcacc tggacctgct
     3541 gatggtgggc gtgatgctgg gcgtgtgcag cgtgatggga ctgccatggt tcgtggcagc
     3601 aaccgtgctg tctatcagcc acgtgaactc cctgaaggtg gagtccgagt gttctgcccc
     3661 aggcgagcag cccaagtttc tgggcatccg ggagcagaga gtgaccggcc tgatgatctt
     3721 catcctgatg ggcctgagcg tgttcatgac atccgtgctg aagtttatcc ctatgccagt
     3781 gctgtacggc gtgtttctgt atatgggcgt gagctccctg aagggcatcc agctgttcga
     3841 ccgcatcaag ctgtttggca tgcctgccaa gcaccagcca gatctgatct acctgagata
     3901 tgtgcctctg tggaaggtgc acatcttcac agtgatccag ctgacctgcc tggtgctgct
     3961 gtgggtcatc aaggtgagcg ccgcagcagt ggtgttccct atgatggtgc tggccctggt
     4021 gtttgtgagg aagctgatgg acctgtgctt caccaagcgc gagctgtcct ggctggatga
     4081 cctgatgcca gagagcaaga agaagaagga ggatgacaag aagaagaagg agaaggagga
     4141 ggccgagaga atgctgcagg atgacgatga cacagtgcac ctgccttttg agggcggctc
     4201 cctgctgcag atcccagtga aggccctgaa gtactctcct gacaagccag tgtctgtgaa
     4261 gatcagcttc gaggacgagc ccaggaagaa gtatgtggat gccgagacca gcctggaccc
     4321 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     4381 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     4441 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     4501 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     4561 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     4621 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     4681 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     4741 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     4801 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     4861 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     4921 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     4981 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     5041 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     5101 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     5161 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     5221 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     5281 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     5341 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     5401 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     5461 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     5521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6181 tgctcacatg tt
//
