LOCUS       sequence-26121-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Liszt G, Ford E, Kurtev M, Guarente L
  TITLE     Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase.
  JOURNAL   J Biol Chem. 2005 Jun 3. 280(22):21313-20.
  PUBMED    15795229
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Jun 3. 280(22):21313-20."
COMMENT     Sequence Label: pET28a-Hisx6-Sirt6
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggcta gaatgtcggt
      121 gaattatgca gcagggttgt cgccttacgc ggataagggc aagtgcgggc tgcccgagat
      181 cttcgaccca ccagaggagc tggaacgcaa ggtgtgggag ctggcccggc taatgtggca
      241 gtcctccagc gtggttttcc acacgggcgc cggcatcagc accgcctctg gcatccccga
      301 cttcagaggc ccccatggcg tgtggaccat ggaggaacgc ggcctggccc ccaagtttga
      361 caccaccttc gagaatgctc ggccctcgaa gacccacatg gccctggttc agctagaacg
      421 catgggcttc ctcagcttcc tggtcagcca gaacgtagac gggctgcacg tgcgctcggg
      481 cttccccagg gacaagctgg cagagctgca cggaaacatg tttgtagagg aatgtcccaa
      541 gtgtaagacg cagtacgtca gagacacggt tgtgggcacc atgggcctca aggccacagg
      601 ccggctctgc accgtggcca agaccagggg acttcgggcc tgtagagggg agctgagaga
      661 caccattctg gactgggagg actcgttgcc tgacc
//
