LOCUS       sequence-261215-        4485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC9A9
FEATURES             Location/Qualifiers
     source          1..4485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2610..2709)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2726..2745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2727..2745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2750..2766)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2750..2766)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2868..2887)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2879..3688
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2946..2965)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3835..4423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4324..4343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggaga ggcagtctcg cgtgatgagc gagaaggacg agtaccagtt
      721 tcagcaccag ggagcagtgg agctgctggt gttcaacttt ctgctgatcc tgaccatcct
      781 gacaatctgg ctgttcaaga atcaccggtt cagatttctg cacgagaccg gcggcgccat
      841 ggtgtatggc ctgatcatgg gactgatcct gagatacgca accgcaccaa cagacatcga
      901 gagcggaacc gtgtacgatt gcgtgaagct gaccttcagc ccttccacac tgctggtgaa
      961 catcacagac caggtgtacg agtataagta caagcgcgag atctcccagc acaacatcaa
     1021 tccccaccag ggcaacgcca tcctggagaa gatgaccttt gatcctgaga tcttctttaa
     1081 tgtgctgctg ccccctatca tcttccacgc cggctattcc ctgaagaagc ggcacttctt
     1141 tcagaacctg ggctctatcc tgacctacgc ctttctgggc acagccatca gctgcatcgt
     1201 gatcggcctg atcatgtatg gcttcgtgaa ggccatgatc cacgccggcc agctgaagaa
     1261 tggcgacttc cactttaccg attgtctgtt ctttggcagc ctgatgtccg ccaccgaccc
     1321 agtgacagtg ctggccatct ttcacgagct gcacgtggac cccgatctgt acaccctgct
     1381 gttcggagag tccgtgctga acgacgcagt ggccatcgtg ctgacatatt ctatcagcat
     1441 ctacagccca aaggagaacc ccaatgcctt cgatgccgcc gccttctttc agtccgtggg
     1501 caatttcctg ggcatctttg ccggctcctt cgccatgggc tctgcctatg ccatcatcac
     1561 cgccctgctg accaagttta caaagctgtg cgagttcccc atgctggaga caggcctgtt
     1621 ctttctgctg tcctggtctg cctttctgtc cgccgaggca gcaggactga ccggaatcgt
     1681 ggccgtgctg ttctgtggcg tgacccaggc ccactataca tacaacaatc tgagctccga
     1741 ttctaagatc aggaccaagc agctgttcga gtttatgaac tttctggccg agaatgtgat
     1801 cttctgctac atgggcctgg ccctgttcac atttcagaac cacatcttta atgccctgtt
     1861 catcctgggc gcctttctgg ccatcttcgt ggcccgggcc tgtaacatct atcctctgag
     1921 ctttctgctg aatctgggca gaaagcagaa gatcccatgg aactttcagc acatgatgat
     1981 gttctctgga ctgaggggag caatcgcctt cgccctggcc atccgcaata ccgagagcca
     2041 gcccaagcag atgatgttta ccacaaccct gctgctggtg ttctttaccg tgtgggtgtt
     2101 cggaggagga acaaccccaa tgctgacatg gctgcagatc agggtgggag tggacctgga
     2161 tgagaacctg aaggaggacc cctctagcca gcaccaggag gccaacaatc tggataagaa
     2221 tatgacaaag gccgagtctg cccggctgtt tagaatgtgg tatagcttcg accacaagta
     2281 cctgaagcct atcctgaccc actctggccc acccctgaca accacactgc ctgagtggtg
     2341 tggcccaatc agccggctgc tgacatcccc acaggcctac ggcgagcagc tgaaggagga
     2401 cgatgtggag tgcatcgtga accaggatga gctggccatc aattatcagg agcaggcctc
     2461 ctctccttgt tcccctccag ccagactggg cctggaccag aaggcctctc cccagacccc
     2521 tggcaaggag aacatctatg agggcgatct gggactggga ggatacgagc tgaagctgga
     2581 gcagacactg ggccagagcc agctgaatga cccagctttc ttgtacaaag ttggcattat
     2641 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2701 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2761 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2821 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     2881 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     2941 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     3001 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     3061 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     3121 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     3181 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     3241 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     3301 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     3361 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3421 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3481 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3541 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3601 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3661 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3721 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     3781 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3841 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3901 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3961 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4021 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4081 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4141 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4201 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4261 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4381 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4441 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
