LOCUS       sequence-261219-       12771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, 
            Calanni ST, Jans DA, Sutherland RL, Watts CK
  TITLE     EDD, the human hyperplastic discs protein, has a role in 
            progesterone receptor coactivation and potential involvement in DNA 
            damage response.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
  PUBMED    12011095
REFERENCE   2  (bases 1 to 12771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-07-19"; volume: "277(29)"; pages: "26468-78. 
            Epub 2002 May 13"
COMMENT     Sequence Label: pCMV-Tag2B EDD C2768A
FEATURES             Location/Qualifiers
     source          1..12771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9166..9183
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9200..9216)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9201..9217)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(9272..9291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9273..9291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    9565..9686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9602..9621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9656..9675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9693..10148)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9830..9851)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10042..10061
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10175..10279
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10281..10638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10281..10472
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10304..10324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10489..10624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10673..11467
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10673..11467
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10727..10746)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11337..11356
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11655..11674)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11699..11746
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12075..12663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12564..12583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc
      721 cccgggctgc aggaattcga tcgagtggag gacgagaagg aaagcaccat gacgtccatc
      781 catttcgtgg ttcacccgct gccgggcacc gaggaccagc tcaatgacag gttacgagaa
      841 gtttctgaga agctgaacaa atataattta aacagccacc cccctttgaa tgtattggaa
      901 caggctacta ttaaacagtg tgtggtggga ccaaatcatg ctgcctttct tcttgaggat
      961 ggtagagttt gcaggattgg tttttcagta cagccagaca gattggaatt gggtaaacct
     1021 gataataatg atgggtcaaa gttgaacagc aactcggggg cagggaggac gtcaaggcct
     1081 ggtaggacaa gcgactctcc atggtttctc tcaggttctg agactctagg caggctggca
     1141 ggcaacacct taggaagccg ctggagttct ggagtgggtg gaagtggtgg aggatcctct
     1201 ggtaggtcat cagctggagc tcgagattcc cgccggcaga ctcgagttat tcggacagga
     1261 cgggatcgag ggtctgggct tttgggcagt cagccccagc cagttattcc agcatctgtc
     1321 attccagagg agctgatttc acaggcccaa gttgttttac aaggcaaatc cagaagtgtc
     1381 attattcgag aacttcagag aacaaatctt gatgtgaacc ttgctgtaaa taatttactt
     1441 agccgggatg atgaagatgg agatgatggg gatgatacag ccagcgaatc ttatttgcct
     1501 ggagaggatc ttatgtctct ccttgatgcc gacattcatt ctgcccaccc aagtgtcatt
     1561 attgatgcag atgccatgtt ttctgaagac attagctatt ttggttaccc ttcttttcgt
     1621 cgttcatcac tttccaggct aggctcatct cgagttctcc ttcttccctt agagagagac
     1681 tctgagctgt tgcgtgaacg tgaatccgtt ttacgtttac gtgaacgaag gtggcttgat
     1741 ggagcctcat ttgataatga aaggggttct accagcaagg aaggagagcc aaacttggat
     1801 aagaagaata cacctgttca aagtccagta tctctaggag aagatttgca gtggtggcct
     1861 gataaggatg gaacaaaatt catctgtatt ggggctctgt attctgaact tctggctgtc
     1921 agcagtaaag gagaacttta tcagtggaaa tggagtgaat ctgagcctta cagaaatgcc
     1981 cagaatcctt cattacatca tccacgagca acatttttgg ggttaaccaa tgaaaagata
     2041 gtcctcctgt ctgcaaatag cataagagca actgtagcta cagaaaataa caaggttgct
     2101 acatgggtgg atgaaacttt aagttctgtg gcttctaaat tagagcacac tgctcagact
     2161 tactctgaac ttcaaggaga gcggatagtt tctttacatt gctgtgccct ttacacctgc
     2221 gctcagctgg aaaacagttt atattggtgg ggtgtagttc cttttagtca aaggaggaaa
     2281 atgttagaga aagctagagc aaaaaataaa aagcctaaat ccagtgctgg tatttcttca
     2341 atgccgaaca tcactgttgg tacccaggta tgcttgagaa ataatcctct ttatcatgct
     2401 ggagcagttg cattttcaat tagtgctggg attcctaaag ttggtgtctt aatggagtca
     2461 gtttggaata tgaatgacag ctgtagattt caacttagat ctcctgaaag cttgaaaaac
     2521 atggaaaaag ctagcaaaac tactgaagct aagcctgaaa gtaagcagga gccagtgaaa
     2581 acagaaatgg gtcctccacc atctccagca tccacgtgta gtgatgcatc ctcaattgcc
     2641 agcagtgcat caatgccata caaacgacga cggtcaaccc ctgcaccaaa agaagaggaa
     2701 aaggtgaatg aagagcagtg gtctcttcgg gaagtggttt ttgtggaaga tgtcaagaat
     2761 gttcctgttg gcaaggtgct aaaagtagat ggtgcctatg ttgctgtaaa atttccagga
     2821 acctccagta atactaactg tcagaacagc tctggtccag atgctgaccc ttcttctctc
     2881 ctgcaggatt gtaggttact tagaattgat gaattgcagg ttgtcaaaac tggtggaaca
     2941 ccgaaggttc ccgactgttt ccaaaggact cctaaaaagc tttgtatacc tgaaaaaaca
     3001 gaaatattag cagtgaatgt agattccaaa ggtgttcatg ctgttctgaa gactggaaat
     3061 tgggtacgat actgtatctt tgatcttgct acaggaaaag cagaacagga aaataatttt
     3121 cctacaagca gcattgcttt ccttggtcag aatgagagga atgtagccat tttcactgct
     3181 ggacaggaat ctcccattat tcttcgagat ggaaatggta ccatctaccc aatggccaaa
     3241 gattgcatgg gaggaataag ggatcccgat tggctggatc ttccacctat tagtagtctt
     3301 ggaatgggtg tgcattcttt aataaatctt cctgccaatt caacaatcaa aaagaaagct
     3361 gctgttatca tcatggctgt agagaaacaa accttaatgc aacacattct gcgctgtgac
     3421 tatgaggcct gtcgacaata tctaatgaat cttgagcaag cggttgtttt agagcagaat
     3481 ctacagatgc tgcagacatt catcagccac agatgtgatg gaaatcgaaa tattttgcat
     3541 gcttgtgtat cagtttgctt tccaaccagc aataaagaaa ctaaagaaga agaggaagcg
     3601 gagcgttctg aaagaaatac atttgcagaa aggctttctg ctgttgaggc cattgcaaat
     3661 gcaatatcag ttgtttcaag taatggccca ggtaatcggg ctggatcatc aagtagccga
     3721 agtttgagat tacgggaaat gatgagacgt tcgttgagag cagctggttt gggtagacat
     3781 gaagctggag cttcatccag tgaccaccag gatccagttt caccccccat agctccccct
     3841 agttgggttc ctgaccctcc tgcgatggat cctgatggtg acattgattt tatcctggcc
     3901 cccgctgtgg gatctcttac cacagcagca accggtactg gtcaaggacc aagcacctcc
     3961 actattccag gtccttccac agagccatct gtagtagaat ccaaggatcg aaaggcgaat
     4021 gctcatttta tattgaaatt gttatgtgac agtgtggttc tccagcccta tctacgagaa
     4081 cttctttctg ccaaggatgc aagagggatg accccattta tgtcagctgt aagtggccga
     4141 gcttatcctg ctgcaattac catcttagaa actgctcaga aaattgcaaa agctgaaata
     4201 tcctcaagtg aaaaagagga agatgtattc atgggaatgg tttgcccatc aggtaccaac
     4261 cctgatgact ctcctttata tgttttatgt tgtaatgaca cttgcagttt tacatggact
     4321 ggagcagagc acattaacca ggatattttt gagtgtcgaa cttgtggctt gctggagtca
     4381 ctgtgttgtt gtacggaatg tgcaagggtt tgtcataaag gtcatgattg caaactcaaa
     4441 cggacatcac caacagccta ctgtgattgt tgggagaaat gtaaatgtaa aactcttatt
     4501 gctggacaga aatctgctcg tcttgatcta ctttatcgcc tgctcactgc tactaatctg
     4561 gttactctgc caaacagcag gggagagcac ctcttactat tcttagtaca gacagtcgca
     4621 aggcagacgg tggagcattg tcaatacagg ccacctcgaa tcagggaaga tcgtaaccga
     4681 aaaacagcca gtcctgaaga ttcagatatg ccagatcatg atttggagcc tccaagattt
     4741 gcccagcttg cattggagcg tgttctacag gactggaatg ccttgaaatc tatgattatg
     4801 tttgggtcgc aggagaataa agaccctctt agtgccagca gtagaatagg ccatcttttg
     4861 ccagaagagc aagtatacct caatcagcaa agtggcacaa ttcggctgga ctgtttcact
     4921 cattgcctta tagttaagtg tacagcagat attttgcttt tagatactct actaggtaca
     4981 ctagtgaaag aactccaaaa caaatataca cctggacgta gagaagaagc tattgctgtg
     5041 acaatgaggt ttctacgttc agtggcaaga gtttttgtta ttctgagtgt ggaaatggct
     5101 tcatccaaaa agaaaaacaa ctttattcca cagccaattg gaaaatgcaa gcgtgtattc
     5161 caagcattgc taccttacgc tgtggaagaa ttgtgcaacg tagcagagtc actgattgtt
     5221 cctgtcagaa tggggattgc tcgtccaact gcaccattta ccctggctag tactagcata
     5281 gatgccatgc agggcagtga agaattattt tcagtggaac cactaccacc acgaccatca
     5341 tctgatcagt ctagcagctc cagtcagtct cagtcatcct acatcatcag gaatccacag
     5401 cagaggcgca tcagccagtc acagcccgtt cggggcagag atgaagaaca ggatgatatt
     5461 gtttcagcag atgtggaaga ggttgaggtg gtggagggtg tggctggaga agaggatcat
     5521 catgatgaac aggaagaaca cggggaagaa aatgctgagg cagagggaca acatgatgag
     5581 catgatgaag acgggagtga tatggagctg gacttgttag cagcagctga aacagaaagt
     5641 gatagtgaaa gtaaccacag caaccaagat aatgctagtg ggcgcagaag cgttgtcact
     5701 gcagcaactg ctggttcaga agcaggagca agcagtgttc ctgccttctt ttctgaagat
     5761 gattctcaat cgaatgactc aagtgattct gatagcagta gtagtcagag tgacgacata
     5821 gaacaggaga cctttatgct tgatgagcca ttagaaagaa ccacaaatag ctcccatgcc
     5881 aatggtgctg cccaagctcc ccgttcaatg cagtgggctg tccgcaacac ccagcatcag
     5941 cgagcagcca gtacagcccc ttccagtaca tctacaccag cagcaagttc agcgggtttg
     6001 atttatattg atccttcaaa cttacgccgg agtggtacca tcagtacaag tgctgcagct
     6061 gcagcagctg ctttggaagc tagcaacgcc agcagttacc taacatctgc aagcagttta
     6121 gccagggctt acagcattgt cattagacaa atctcggact tgatgggcct tattcctaag
     6181 tataatcacc tagtatactc tcagattcca gcagctgtga aattgactta ccaagatgca
     6241 gtaaacttac agaactatgt agaagaaaag cttattccca cttggaactg gatggtcagt
     6301 attatggatt ctactgaagc tcaattacgt tatggttctg cattagcatc tgctggtgat
     6361 cctggacatc caaatcatcc tcttcacgct tctcagaatt cagcgagaag agagaggatg
     6421 actgcgcgag aagaagctag cttacgaaca cttgaaggca gacgacgtgc caccttgctt
     6481 agcgcccgtc aaggaatgat gtctgcacga ggagacttcc taaattatgc tctgtctcta
     6541 atgcggtctc ataatgatga gcattctgat gttcttccag ttttggatgt ttgctcattg
     6601 aagcatgtgg catatgtttt tcaagcactt atatactgga ttaaggcaat gaatcagcag
     6661 acaacattgg atacacctca actagaacgc aaaaggacgc gagaactctt ggaactgggt
     6721 attgataatg aagattcaga acatgaaaat gatgatgaca ccaatcaaag tgctactttg
     6781 aatgataagg atgatgactc tcttcctgca gaaactggcc aaaaccatcc atttttccga
     6841 cgttcagact ccatgacatt ccttgggtgt atacccccaa atccatttga agtgcctctg
     6901 gctgaagcca tccccttggc tgatcagcca catctgttgc agccaaatgc tagaaaggag
     6961 gatctttttg gccgtccaag tcagggtctt tattcttcat ctgccagtag tgggaaatgt
     7021 ttaatggagg ttacagtgga tagaaactgc ctagaggttc ttccaacaaa aatgtcttat
     7081 gctgccaatc tgaaaaatgt aatgaacatg caaaaccggc aaaaaaaaga aggggaagaa
     7141 cagcccgtgc tgccagaaga aactgagagt tcaaaaccag ggccatctgc tcatgatctt
     7201 gctgcacaat taaaaagtag cttactagca gaaataggac ttactgaaag tgaagggcca
     7261 cctctcacat ctttcaggcc acagtgtagc tttatgggaa tggttatttc ccatgatatg
     7321 ctgctaggac gttggcgcct ttctttagaa ctgttcggca gggtattcat ggaagatgtt
     7381 ggagcagaac ctggatcaat cctaactgaa ttgggtggtt ttgaggtaaa agaatcaaaa
     7441 ttccgcagag aaatggaaaa actgagaaac cagcagtcaa gagatttgtc actagaggtt
     7501 gatcgggatc gagatcttct cattcagcag actatgaggc agcttaacaa tcactttggt
     7561 cgaagatgtg ctactacacc aatggctgta cacagagtaa aagtcacatt taaggatgag
     7621 ccaggagagg gcagtggtgt agcacgaagt ttttatacag ccattgcaca agcattttta
     7681 tcaaatgaaa aattgccaaa tctagagtgt atccaaaatg ccaacaaagg cacccacaca
     7741 agtttaatgc agagattaag gaaccgagga gagagagacc gggaaaggga gagagaaagg
     7801 gaaatgagga ggagtagtgg tttgcgagca ggttctcgga gggaccggga tagagacttt
     7861 agaagacagc tttccatcga cactaggccc tttagaccag cctctgaagg gaatcctagc
     7921 gatgatcctg agcctttgcc agcacatcgg caggcacttg gagagaggct ttatcctcgt
     7981 gtacaagcaa tgcaaccagc atttgcaagt aaaatcactg gcatgttgtt ggaattatcc
     8041 ccagctcagc tgcttctcct tctagcaagt gaggattctc tgagagcaag agtggatgag
     8101 gccatggaac tcattattgc acatggacgg gaaaatggag ctgatagtat cctggatctt
     8161 ggattagtag actcctcaga aaaggtacag caggaaaacc gaaagcgcca tggctctagt
     8221 cgaagtgtag tagatatgga tttagatgat acagatgatg gtgatgacaa tgcccctttg
     8281 ttttaccaac ctgggaaaag aggattttat actccaaggc ctggcaagaa cacagaagca
     8341 aggttgaatt gtttcagaaa cattggcagg attcttggac tatgtctgtt acagaatgaa
     8401 ctctgtccta tcacattgaa tagacatgta attaaagtat tgcttggtag aaaagtcaat
     8461 tggcatgatt ttgctttttt tgatcctgta atgtatgaga gtttgcggca actaatcctc
     8521 gcgtctcaga gttcagatgc tgatgctgtt ttctcagcaa tggatttggc atttgcaatt
     8581 gacctgtgta aagaagaagg tggaggacag gttgaactca ttcctaatgg tgtaaatata
     8641 ccagtcactc cacagaatgt atatgagtat gtgcggaaat acgcagaaca cagaatgttg
     8701 gtagttgcag aacagccctt acatgcaatg aggaaaggtc tactagatgt gcttccaaaa
     8761 aattcattag aagatttaac ggcagaagat tttaggcttt tggtaaatgg ctgcggtgaa
     8821 gtcaatgtgc aaatgctgat cagttttacc tctttcaatg atgaatcagg agaaaatgct
     8881 gagaagcttc tgcagttcaa gcgttggttc tggtcaatag tagagaagat gagcatgaca
     8941 gaacgacaag atcttgttta cttttggaca tcaagcccat cactgccagc cagtgaagaa
     9001 ggattccagc ctatgccctc aatcacaata agaccaccag atgaccaaca tcttcctact
     9061 gcaaatactg ccatttctcg actttacgtc ccactctatt cctctaaaca gattctcaaa
     9121 cagaaattgt tactcgccat taagaccaag aattttggtt ttgtgcatca ccatcaccat
     9181 cactagccat caagcttatc gataccgtcg acctcgaggg ggggcccggt accttaatta
     9241 attaaggtac caggtaagtg tacccaattc gccctatagt gagtcgtatt acaattcact
     9301 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggagatcca atttttaagt
     9361 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattca cagtcccaag
     9421 gctcatttca ggcccctcag tcctcacagt ctgttcatga tcataatcag ccataccaca
     9481 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     9541 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     9601 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9661 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     9721 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     9781 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     9841 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     9901 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     9961 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
    10021 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
    10081 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
    10141 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10261 atattgaaaa aggaagaatc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
    10321 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    10381 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10441 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10561 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10621 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
    10681 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
    10741 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
    10801 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
    10861 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
    10921 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
    10981 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
    11041 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
    11101 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagaacatca
    11161 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
    11221 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
    11281 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
    11341 ggctacccgt gatattgctg aagaacttgg cggcgaatgg gctgaccgct tcctcgtgct
    11401 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
    11461 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
    11521 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    11581 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
    11641 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
    11701 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
    11761 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
    11821 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
    11881 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
    11941 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    12001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    12061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    12121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    12181 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    12241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    12301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    12361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    12421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    12481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    12541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    12601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    12661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    12721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc c
//
