LOCUS       sequence-26122-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Liszt G, Ford E, Kurtev M, Guarente L
  TITLE     Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase.
  JOURNAL   J Biol Chem. 2005 Jun 3. 280(22):21313-20.
  PUBMED    15795229
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Jun 3. 280(22):21313-20."
COMMENT     Sequence Label: pET28a-Hisx6-Sirt6-S56A
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctagaa tgtcggtgaa ttatgcagca
      121 gggttgtcgc cttacgcgga taagggcaag tgcgggctgc ccgagatctt cgacccacca
      181 gaggagctgg aacgcaaggt gtgggagctg gcccggctaa tgtggcagtc ctccagcgtg
      241 gttttccaca cgggcgccgg catcgcgacc gcctctggca tccccgactt cagaggcccc
      301 catggcgtgt ggaccatgga ggaacgcggc ctggccccca agtttgacac caccttcgag
      361 aatgctcggc cctcgaagac ccacatggcc ctggttcagc tagaacgcat gggcttcctc
      421 agcttcctgg tcagccagaa cgtagacggg ctgcacgtgc gctcgggctt ccccagggac
      481 aagctggcag agctgcacgg aaacatgttt gtagaggaat gtcccaagtg taagacgcag
      541 tacgtcagag acacggttgt gggcaccatg ggcctcaagg ccacaggccg gctctgcacc
      601 gtggccaaga ccaggggact tcgggcctgt agaggggagc tgagagacac cattctggac
      661 tgggaggact cgttgcctga ccgggacctg atgctcgctg atgaggccag caggaccgca
      721 gacctgtctg tcaccctggg gtac
//
