LOCUS       sequence-261239-       12415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single component LSL vector with GFP. Rep is expressed from the 
            complementary sense LIR promoter. GFP is between LIR and SIR and is 
            driven from a 2X35S promoter. Rep is within the replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLGFP.R
FEATURES             Location/Qualifiers
     source          1..12415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1125..1319
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1555..1577)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1663..1803
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1718..1737
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1818..1835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1989..2577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2069..2088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2664..3458)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3883..3907
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    3985..4159
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(5249..5925)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        6941..7286
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7334..7362)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7957..7977
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      8036..8288
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10018..10042
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11343..11972
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(12401..12415,1..1059)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
       61 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
      121 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
      181 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
      241 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
      301 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
      361 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
      421 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
      481 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
      541 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
      601 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
      661 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
      721 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
      781 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
      841 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
      901 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
      961 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     1021 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     1081 caaattgccc tagcagggga aaaaggtcga aaaggcctct ttcctgtgga tagcacgtac
     1141 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     1201 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     1261 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     1321 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     1381 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     1441 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     1501 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     1561 acacatgcag ctcccggaaa cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     1621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     1681 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     1741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1801 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1861 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2401 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     2461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2641 attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt
     2701 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
     2761 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
     2821 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
     2881 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
     2941 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
     3001 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
     3061 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
     3121 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
     3181 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
     3241 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
     3301 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
     3361 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
     3421 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
     3481 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
     3541 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
     3601 tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa
     3661 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
     3721 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
     3781 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
     3841 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
     3901 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
     3961 aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa
     4021 ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat
     4081 atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc
     4141 acacattatt atggagaaaa tagagcttgt cgatcgttaa ttaatcacct ccaagatccc
     4201 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     4261 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     4321 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     4381 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct
     4441 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     4501 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     4561 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     4621 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     4681 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
     4741 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
     4801 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc
     4861 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
     4921 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc
     4981 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
     5041 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
     5101 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggacacgct gtcgaacttt
     5161 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat cctaggagag
     5221 atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc
     5281 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     5341 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     5401 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     5461 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     5521 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     5581 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     5641 tggagtagac gagagtgtcg tgctccacca tgttcacatc aatccacttg ctttgaagac
     5701 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     5761 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     5821 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     5881 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     5941 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtcg
     6001 actatcggac gagctcaaag tttaacgcgt tagcagaagg catgttgttg tgactccgag
     6061 gggttgcctc aaactctatc ttataaccgg cgtggaggca tggaggcagg ggtattttgg
     6121 tcattttaat agatagtgga aaatgacgtg gaatttactt aaagacgaag tctttgcgac
     6181 aagggggggc ccacgccgaa tttaatatta ccggcgtggc ccccccttat cgcgagtgct
     6241 ttagcacgag cggtccagat ttaaagtaga aaatttcccg cccactaggg ttaaaggtgt
     6301 tcacactata aaagcatata cgatgtgatg gtatttgatg gagcgtatat tgtatcaggt
     6361 atttccgttg gatacgaatt attcgtacga ccctcggcgc gccgagaact tccagagctt
     6421 tgggtacgtc acctgatagt ttaaacggtc cccagatttg ccttttcaat ttcagaaaga
     6481 atgctaaccc acagatggtt agagaggctt acgcagcagg tctcatcaag acgatctacc
     6541 cgagcaataa tctccaggaa atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa
     6601 gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta
     6661 ctattccagt atggacgatt caaggcttgc ttcacaaacc aaggcaagta atagagattg
     6721 gagtctctaa aaaggtagtt cccactgaat caaaggccat ggagtcaaag attcaaatag
     6781 aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt ctcttacgac
     6841 tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacactt gtctactcca
     6901 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
     6961 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga
     7021 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg
     7081 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     7141 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     7201 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     7261 gaagttcatt tcatttggag agaacacggg ggactctaga atggtgagta aaggagaaga
     7321 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     7381 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     7441 tatttgcact actggaaaac tacctgttcc atggccaacc ctggtcacca ccctgaccta
     7501 cggcgtgcag tgcttctccc gttaccctga tcatatgaag cggcacgact tcttcaagag
     7561 cgccatgcct gagggatacg tgcaggagag gaccatcttc ttcaaggacg acgggaacta
     7621 caagacacgt gctgaagtca agtttgaggg agacaccctc gtcaacagga tcgagcttaa
     7681 gggaatcgat ttcaaggagg acggaaacat cctcggccac aagttggaat acaactacaa
     7741 ctcccacaac gtatacatca tggccgacaa gcaaaagaac ggcatcaaag ccaacttcaa
     7801 gacccgccac aacatcgaag acggcggcgt gcaactcgct gatcattatc aacaaaatac
     7861 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     7921 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     7981 tgctgggatt acacatggca tggatgaact atacaaataa agctcgaatt tccccgatcg
     8041 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     8101 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     8161 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     8221 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     8281 actagatcgt gggcccagct ccagacgtcg cggtggtggc tcgagcgcgt agtcctcggt
     8341 aatatcgcag aacaaaagta cctgatatcg agtgtacttc aagtcagtgg gaaatcaata
     8401 aaatgattat tttatgaata tatttcattg tgcaagtaga tagaaattac atatgttaca
     8461 taacacacga aataaacaaa aaaagacaat ccaaaaacaa acaccccaaa aaaaataatc
     8521 actttagata aactcgtatg aggagaggca cgttcagtga ctcgacgatt cccgagcaaa
     8581 aaaagtctcc ccgtcacaca tgtagtgggt gacgcaatta tctttaaagt aatccttctg
     8641 ttgacttgtc attgataaca tccagtcttc gtcaggattg caaagaatta tagaagggat
     8701 cccacctttt attttcttct tttttccata tttagggttg acagtgaaat cagactggca
     8761 acctattaat tgcttccaca atgggacgaa cttgaagggg atgtcgtcga tgatattata
     8821 ggtggcgtgt tcatcgtagt tggtgaaatc gatggtaccg ttccaatagt tgtgtcgtcc
     8881 gagacttcta gcccaggtgg tctttccggt acgagttggt ccgcagatgt agaggctggg
     8941 gtgtcggatt ccattccttc cattgtcctt gttaaatcgg ccatccattc aaggtcagat
     9001 tgagcttgtt ggtatgagac aggatgtatg taagtataag cgtctatgct tacatggtat
     9061 agatgggttt ccctccagga gtgtagatct tcgtggcagc gaagatctga ttctgtgaag
     9121 ggcgacacat acggttcagg ttgtggaggg aataatttgt tggctgaata ttccagccat
     9181 tgaagctttg ttgcccattc atgagggaat tcttccttga tcatgtcaag atattcctcc
     9241 ttagacgttg cagtctggat aatagttctc catcgtgcgt cagatttgcg aggagaaacc
     9301 ttatgatctc ggaaatctcc tctggtttta atatctccgt cctttgatat gtaatcaagg
     9361 acttgtttag agtttctagc tggctggata ttagggtgat ttccttcaaa atcgaaaaaa
     9421 gaaggatccc taatacaagg ttttttatca agctggagaa gagcatgata gtgggtagtg
     9481 ccatcttgat gaagctcaga agcaacacca aggaagaaaa taagaaaagg tgtgagtttc
     9541 tcccagagaa actggaataa atcatctctt tgagatgagc acttgggata ggtaaggaaa
     9601 acatatttag attggagtct gaagttctta ctagcagaag gcatgttgtt gtgactccga
     9661 ggggttgcct caaactctat cttataaccg gcgtggaggc atggaggcag gggtattttg
     9721 gtcattttaa tagatagtgg aaaatgacgt ggaatttact taaagacgaa gtctttgcga
     9781 caaggggggg cccacgccga atttaatatt accggcgtgg ccccccctta tcgcgagtgc
     9841 tttagcacga gcggtccaga tttaaagtag aaaatttccc gcccactagg gttaaaggtg
     9901 ttcacactat aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg
     9961 tatttccgtt ggatacgaat tattcgtacg accctcatag tttaaactat cagtgtttga
    10021 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
    10081 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
    10141 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
    10201 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
    10261 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
    10321 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
    10381 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
    10441 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
    10501 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
    10561 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
    10621 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
    10681 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
    10741 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
    10801 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
    10861 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
    10921 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
    10981 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
    11041 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
    11101 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acggctcaac cgtgcggctg
    11161 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
    11221 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
    11281 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
    11341 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
    11401 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
    11461 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
    11521 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
    11581 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
    11641 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
    11701 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
    11761 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
    11821 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
    11881 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
    11941 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
    12001 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
    12061 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
    12121 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
    12181 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
    12241 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
    12301 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc
    12361 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaa
//
