LOCUS       sequence-261240-        8893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA
  TITLE     Differential WNT activity in colorectal cancer confers limited 
            tumorigenic potential and is regulated by MAPK signaling.
  JOURNAL   Cancer Res. 2012 Feb 8.
  PUBMED    22318865
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2012 Feb 8."
COMMENT     Sequence Label: pUG2K
FEATURES             Location/Qualifiers
     source          1..8893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1833..1950
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2015..3226
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3063..3082
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3259..3268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3265..3267,3268..3270,3271..3981)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3265..3267,3268..3270,3271..3981)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3310..3331)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3571..3590)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3918..3939
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3991..4047
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4054..4086,4087..4089,4090..4620)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             4636..4689
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4733..5321
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4786..4806)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5204..5215)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5412..5645
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5717..5838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5754..5773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5808..5827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5878..6013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6033..6052)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6034..6052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6062..6079)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6062..6078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6071..6093)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6220..6675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6307..6326)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6517..6538
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6701..6805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6806..6874,6875..7666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7024..7043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7837..8425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8326..8345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8579..8596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8713..8734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8749..8754,8755..8772,8773..8779)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8787..8803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8792..8814
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8811..8827
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8811..8827
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8846..8866
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8848..8866
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1801 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1861 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     1921 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     1981 gtttggttaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt tttggcgcct
     2041 cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag
     2101 cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc
     2161 ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca
     2221 ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct
     2281 gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg
     2341 gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt
     2401 gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc
     2461 cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag
     2521 caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt
     2581 cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat
     2641 ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg
     2701 ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag
     2761 aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac
     2821 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta
     2881 taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac
     2941 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg
     3001 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta
     3061 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt
     3121 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
     3181 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag cttgggctgc
     3241 aggtccgatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3301 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3361 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3421 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3481 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3541 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3601 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3661 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3721 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3781 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3841 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3901 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3961 ggcatggacg agctgtacaa gggatctgga gcaacaaact tctcactact caaacaagca
     4021 ggtgacgtgg aggagaatcc cgggccttct agaatgactg aatataaact tgtggtagtt
     4081 ggagctgttg gcgtaggcaa gagtgccttg acgatacagc taattcagaa tcattttgtg
     4141 gacgaatatg atccaacaat agaggattcc tacaggaagc aagtagtaat tgatggagaa
     4201 acctgtctct tggatattct cgacacagca ggtcaagagg agtacagtgc aatgagggac
     4261 cagtacatga ggactgggga gggctttctt tgtgtatttg ccataaataa tactaaatca
     4321 tttgaagata ttcaccatta tagagaacaa attaaaagag ttaaggactc tgaagatgta
     4381 cctatggtcc tagtaggaaa taaatgtgat ttgccttcca gaacagtaga cacaaaacag
     4441 gctcaggact tagcaagaag ttatggaatt ccttttattg aaacatcagc aaagacaaga
     4501 cagggtgttg atgatgcctt ctatacatta gttcgagaaa ttcgaaaaca taaagaaaag
     4561 atgagcaaag atggtaaaaa gaagaaaaag aagtcaaaga caaagtgtgt aattatgtaa
     4621 ggatccggct ccggcgaggg caggggaagt ctactaacat gcggggacgt ggaggaaaat
     4681 cccggcccag ctagcgtgtg atcagaattc gttccggagt cgtcgactcg acaatcaacc
     4741 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4801 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4861 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4921 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4981 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5041 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5101 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     5161 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5221 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcatc ttcgccttcg
     5281 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attcgagctc
     5341 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5401 aaagggggga ctggaagggc tacgtaactc ccaacgaaga caagatctgc tttttgcttg
     5461 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5521 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5581 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5641 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     5701 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5761 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5821 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     5881 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5941 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6001 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6061 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6121 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6181 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6241 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6301 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6361 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6421 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6481 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6541 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6601 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6661 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6841 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6901 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6961 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7021 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7081 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7141 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7201 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7261 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7321 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7381 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7681 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7741 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7801 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7861 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7921 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7981 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8041 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8101 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8161 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8221 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8281 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8341 tctttatagt cctgtcgggt ttcgccacct ctcacttgag cgtcgatttt tgtgatgctc
     8401 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8461 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8521 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8581 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8641 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     8701 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8761 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     8821 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     8881 gctgcaagct taa
//
