LOCUS       sequence-261245-        3898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0626
FEATURES             Location/Qualifiers
     source          1..3898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             433..1143
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      1159..1346
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1214..1238)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(1663..2454,2455..2523))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2286..2305
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2524..2628)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2731..3319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3220..3239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaagtaaaaa aaaatgggac cacgtcttga gaacgataga ttttctttat tttacattga
       61 acagtcgttg tctcagcgcg ctttatgttt tcattcatac ttcatattat aaaataacaa
      121 aagaagaatt tcatattcac gcccaagaaa tcaggctgct ttccaaatgc aattgacact
      181 tcattagcca tcacacaaaa ctctttcttg ctggagcttc ttttaaaaaa gacctcagta
      241 caccaaacac gttacccgac ctcgttattt tacgacaact atgataaaat tctgaagaaa
      301 aaataaaaaa attttcatac ttcttgcttt tatttaaacc attgaatgat ttcttttgaa
      361 caaaactacc tgtttcacca aaggaaatag aaagaaaaaa tcaattagaa gaaaacaaaa
      421 aacaaaagat ctatggtgtc caaaggagag gagttaatca aggaaaacat gagaatgaaa
      481 gttgtcatgg agggctccgt taatggtcac caattcaagt gtacagggga aggtgaaggt
      541 aatccttaca tgggtacaca aactatgaga attaaagtaa ttgaaggcgg accactacca
      601 tttgcatttg acattctggc aacgtcattc atgtacggat cacgaacttt catcaagtac
      661 cctaaaggta taccagactt tttcaagcaa tcttttccag agggttttac atgggaaagg
      721 gttacaagat acgaagatgg gggtgtcgtc acagttatgc aagatacttc attagaagat
      781 ggctgccttg tctatcatgt gcaagtaaga ggggtgaatt ttccttctaa cggacctgtg
      841 atgcagaaaa agaccaaagg ttgggaacca aatactgaaa tgatgtaccc agctgatgga
      901 ggtttgagag gctacacaca catggcgctt aaagttgatg gtggaggtca tttgtcttgt
      961 agttttgtta ccacttatcg ttctaaaaag actgttggca atatcaaaat gccaggaata
     1021 catgctgtag accacagact agaaagactc gaagagagcg ataacgaaat gttcgttgta
     1081 cagagagagc atgccgtagc caaatttgct ggcttaggcg gtggtatgga tgaattgtat
     1141 aagggatcct aactcgaggc gaatttctta tgatttatga tttttattat taaataagtt
     1201 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     1261 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
     1321 gctcttattg accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat
     1381 ttcgctgagc atgagacgga aatctgctcg tcagtggtgc tcacactgac gaatcatgta
     1441 cagatcatac cgatgactgc ctggcgactc acaactaagc aagacagccg gaaccagcgc
     1501 cggcgaacac cactgcatat atggcatatc acaacagtcc aactagtgca ctgcagtaca
     1561 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1621 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1681 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1741 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1801 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1861 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1921 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1981 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2041 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2101 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2161 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2221 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2281 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2341 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2401 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2461 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2521 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2581 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2641 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     3421 ggccgcccct gaattcgcat ctagaataaa tcgcagccaa gtgagtgaat agatgacgca
     3481 ccacggtcag acacggccac atcgtatctc acaggagcaa gcgcgatagg agcactcaca
     3541 catagtacgg tgatccgctg actcctttgc ccaaataaga cgtgagcccg tctcagatgc
     3601 acacactggc ttaagatgac aacgaagagg atgtccaata ttttttttaa ggaataagga
     3661 tacttcaaga ctagattccc ccctgcattc ccatcagaac cgtaaacctt ggcgctttcc
     3721 ttgggaagta ttcaagaagt gccttgtccg gtttctgtgg ctcacaaacc agcgcgcccg
     3781 atatggcttt cttttcactt atgaatgtac cagtacggga caattagaac gctcctgtaa
     3841 caatctcttt gcaaatgtgg ggttacattc taaccatgtc acactgctga cgaaattc
//
