LOCUS       sequence-261246-       10759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector for optogenetic machinery 
            (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0638
FEATURES             Location/Qualifiers
     source          1..10759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        213..231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             256..765
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             2285..2305
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2588..4081
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     5012..5035
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             5059..5769
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      5785..5972
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5840..5864)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             6235..7038
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(7680..8495)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7680..8495)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8403..8422
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8487..8506
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8720..9308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9209..9228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10261..10674
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             10724..10744
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcctcggagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac
       61 gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt taccccccac
      121 gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc
      181 gatgcccttg gaattgacga gtacggtggg tttaatacga ctcactatag ggcgagcgcc
      241 gaagctagcg ccaccatgaa tggagctata ggaggtgacc ttttgctcaa ttttcctgac
      301 atgtcggtcc tagagcgcca aagggctcac ctcaagtacc tcaatcccac ctttgattct
      361 cctctcgccg gcttctttgc cgattcttca atgattaccg gcggcgagat ggacagctat
      421 ctttcgactg ccggtttgaa tcttccgatg atgtacggtg aaacgacggt ggaaggtgat
      481 tcaagactct caatttcgcc ggaaacgacg cttgggactg gaaatttcaa gaaacggaag
      541 tttgatacag agactaagga ttgtaatgag aagaagaaga agatgacgat gaacagagat
      601 gacctagtag aagaaggaga agaagagaag tcgaaaataa cagagcaaaa caatgggagc
      661 acaaaaagca tcaagaagat gaaacacaaa gccaagaaag aagagaacaa tttctctaat
      721 gattcatcta aagtgacgaa ggaattggag aaaacggatt atattcatgt tcgtgcacga
      781 cgaggccaag ccactgatag tcacagcata gcagaacgag ttagaagaga aaagatcagt
      841 gagagaatga agtttctaca agatttggtt cctggatgcg acaagatcac aggcaaagca
      901 gggatgcttg atgaaatcat taactatgtt cagtctcttc agagacaaat cgagttctta
      961 tcgatgaaac tagcaattgt gaatccaagg ccggattttg atatggatga catttttgcc
     1021 aaagaggttg cctcaactcc aatgactgtg gtgccatctc ctgaaatggt tctttccggt
     1081 tattctcatg agatggttca ctctggttat tctagtgaga tggttaactc cggttacctt
     1141 catgtcaatc caatgcagca agtgaatacc agttctgatc cattgtcatg cttcaacaat
     1201 ggcgaagctc cttcgatgtg ggactctcat gtgcagaatc tctatggcaa tttaggagta
     1261 ccggtcatcg agctcgagct gcagatgaat cgtagatacg gatcctaact cgagagcttt
     1321 tgattaagcc ttctagtcca aaaaacacgt ttttttgtca tttatttcat tttcttagaa
     1381 tagtttagtt tattcatttt atagtcacga atgttttatg attctatata gggttgcaaa
     1441 caagcatttt tcattttatg ttaaaacaat ttcaggttta ccttttattc tgcttgtggt
     1501 gacgcgtgta tccgcccgct cttttggtca cccatgtatg ctgccaaacc agatgtcaac
     1561 acagctacaa cgaagaggat gtccaatatt ttttttaagg aataaggata cttcaagact
     1621 agattccccc ctgcattccc atcagaaccg taaaccttgg cgctttcctt gggaagtatt
     1681 caagaagtgc cttgtccggt ttctgtggct cacaaaccag cgcgcccgat atggctttct
     1741 tttcacttat gaatgtacca gtacgggaca attagaacgc tcctgtaaca atctctttgc
     1801 aaatgtgggg ttacattcta accatgtcac actgctgacg aaattcaaag taaaaaaaaa
     1861 tgggaccacg tcttgagaac gatagatttt ctttatttta cattgaacag tcgttgtctc
     1921 agcgcgcttt atgttttcat tcatacttca tattataaaa taacaaaaga agaatttcat
     1981 attcacgccc aagaaatcag gctgctttcc aaatgcaatt gacacttcat tagccatcac
     2041 acaaaactct ttcttgctgg agcttctttt aaaaaagacc tcagtacacc aaacacgtta
     2101 cccgacctcg ttattttacg acaactatga taaaattctg aagaaaaaat aaaaaaattt
     2161 tcatacttct tgcttttatt taaaccattg aatgatttct tttgaacaaa actacctgtt
     2221 tcaccaaagg aaatagaaag aaaaaatcaa ttagaagaaa acaaaaaaca aaagatctat
     2281 ggcccccaag aagaagagga aggttggtat tcacgggggt acccgcccat atgcttgccc
     2341 tgtcgagtcc tgcgatcgcc gcttttctcg ctcggatgag cttacccgcc atatccgcat
     2401 ccataccggt cagaagccct tccagtgtcg aatctgcatg cgtaacttca gtcgtagtga
     2461 ccaccttacc acccacatcc gcacccacac aggcgagaag ccttttgcct gtgacatttg
     2521 tgggaggaag tttgccagga gtgatgaacg caagaggcat accaaaatcc atacaggtgc
     2581 tagcttcatg aagatggaca aaaagactat agtttggttt agaagagatc taaggattga
     2641 ggataatcct gcattagcag cagctgctca cgaaggatct gtttttcctg tcttcatttg
     2701 gtgtcctgaa gaagaaggac agttttatcc tggaagagct tcaagatggt ggatgaaaca
     2761 atcacttgct cacttatctc aatccttgaa ggctcttgga tctgacctca ctttaatcaa
     2821 aacccacaac acgatttcag cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt
     2881 cgtctttaac cacctctatg atcctgtttc gttagttcgg gaccataccg taaaggagaa
     2941 gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg
     3001 ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc aattcttact ggaagaaatg
     3061 cttagatatg tcgattgaat ccgttatgct tcctcctcct tggcggttga tgccaataac
     3121 tgcagcggct gaagcgattt gggcgtgttc gattgaagaa ctagggctgg agaatgaggc
     3181 cgagaaaccg agcaatgcgt tgttaactag agcttggagt ccaggatgga gcaatgctga
     3241 taagttacta aatgagttca tcgagaagca gttgatagat tatgcaaaga acagcaagaa
     3301 agttgttggg aattctactt cactactttc tccgtatctc catttcgggg aaataagcgt
     3361 cagacacgtt ttccagtgtg cccggatgaa acaaattata tgggcaagag ataagaacag
     3421 tgaaggagaa gaaagtgcag atctttttct taggggaatc ggtttaagag agtattctcg
     3481 gtatatatgt ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc atcttcggtt
     3541 tttcccttgg gatgctgatg ttgataagtt caaggcctgg agacaaggca ggaccggtta
     3601 tccgttggtg gatgccggaa tgagagagct ttgggctacc ggatggatgc ataacagaat
     3661 aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc cttccatgga aatggggaat
     3721 gaagtatttc tgggatacac ttttggatgc tgatttggaa tgtgacatcc ttggctggca
     3781 gtatatctct gggagtatcc ccgatggcca cgagcttgat cgcttggaca atcccgcgtt
     3841 acaaggcgcc aaatatgacc cagaaggtga gtacataagg caatggcttc ccgagcttgc
     3901 gagattgcca actgaatgga tccatcatcc atgggacgct cctttaaccg tactcaaagc
     3961 ttctggtgtg gaactcggaa caaactatgc gaaacccatt gtagacatcg acacagctcg
     4021 tgagctacta gctaaagcta tttcaagaac ccgtgaagca cagatcatga tcggagcagc
     4081 agcccgggga tcctaactcg aggccaattg gtgcggcaat tgataataac gaaaatgtct
     4141 tttaatgatc tgggtataat gaggaatttt ccgaacgttt ttactttata tatatatata
     4201 catgtaacat atattctata cgctatagag aaaggaaatt tttcaattaa aaaaaaaata
     4261 gagaaagagt ttcacttctt gattatcgct aacactaatg gttgaagtac tgctacttta
     4321 attttatgct ggatgcacac actggcttaa gatgacaacg cgaggcaagc taaacagatc
     4381 tttatattga attttcaaaa attcttactt tttttttgga tggacgcaaa gaagtttaat
     4441 aatcatatta catggcatta ccaccatata catatccata tacatatcca tatctaatct
     4501 tacttatatg ttgtggaaat gtaaagagcc ccattatctt agcctaaaaa aaccttctct
     4561 ttggaacttt cagtaatacg cttaactgct cattgctata ttgaagtgcg cgcgcgcgtg
     4621 ggcgagcgtg ggcgagcgtg ggcgtctaga ccgtgcgtcc tcgtcttcac cggtcgcgtt
     4681 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact
     4741 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat
     4801 taacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct
     4861 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatggaaaa
     4921 gctgcataac cactttaact aatactttca acattttcag tttgtattac ttcttattca
     4981 aatgtcataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga
     5041 gaaaaaacta taggtactat ggtgtccaaa ggagaggagt taatcaagga aaacatgaga
     5101 atgaaagttg tcatggaggg ctccgttaat ggtcaccaat tcaagtgtac aggggaaggt
     5161 gaaggtaatc cttacatggg tacacaaact atgagaatta aagtaattga aggcggacca
     5221 ctaccatttg catttgacat tctggcaacg tcattcatgt acggatcacg aactttcatc
     5281 aagtacccta aaggtatacc agactttttc aagcaatctt ttccagaggg ttttacatgg
     5341 gaaagggtta caagatacga agatgggggt gtcgtcacag ttatgcaaga tacttcatta
     5401 gaagatggct gccttgtcta tcatgtgcaa gtaagagggg tgaattttcc ttctaacgga
     5461 cctgtgatgc agaaaaagac caaaggttgg gaaccaaata ctgaaatgat gtacccagct
     5521 gatggaggtt tgagaggcta cacacacatg gcgcttaaag ttgatggtgg aggtcatttg
     5581 tcttgtagtt ttgttaccac ttatcgttct aaaaagactg ttggcaatat caaaatgcca
     5641 ggaatacatg ctgtagacca cagactagaa agactcgaag agagcgataa cgaaatgttc
     5701 gttgtacaga gagagcatgc cgtagccaaa tttgctggct taggcggtgg tatggatgaa
     5761 ttgtataagg gatcctaact cgaggcgaat ttcttatgat ttatgatttt tattattaaa
     5821 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     5881 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat
     5941 gaggtcgctc ttattgacca cacctctacc ggcatgccga gcaaatgcct gcaaatcgct
     6001 ccccatttcg ctgagcagtt acagagatgt tacgaaccac tagtgcactg cagtacacgg
     6061 tttccttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag
     6121 cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca
     6181 gtattcttaa cccaactgca cagaacaaaa acgtgcagga aacgaagata aatcatgtcg
     6241 aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt
     6301 aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag
     6361 gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg
     6421 gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc
     6481 aagtacaatt ttttactctt cgaggacaga aaatttgctg acattggtaa tacagtcaaa
     6541 ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac
     6601 ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag
     6661 gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga
     6721 gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt
     6781 attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc
     6841 ggtgtgggtt tagatgacaa gggagatgca ttgggtcaac agtatagaac cgtggatgat
     6901 gtggtttcta caggatctga cattattatt gttggaagag gactatttgc aaagggaagg
     6961 gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga
     7021 tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa
     7081 attagagctt caatttaatt atatcagtta ttacccgagt agagcacttg aatccactgc
     7141 cccgggaatc tcggtcgtaa tgatttctat aatgacgaaa aaaaaaaaat tggaaagaaa
     7201 aagcttcatg gcctttataa aaaggaacta tccaatacct cgccagaacc aagtaacagt
     7261 attttacggg gcacaaatca agaacaataa gacaggactg taaagatgga cgcattgaac
     7321 tccaaagaac aacaagagtt ccaaaaagta gtggaacaaa agcaaatgaa ggatttcatg
     7381 cgtttgtact ctaatctggt agaaagatgt ttcacagact gtgtcaatga cttcacaaca
     7441 tcaaagctaa ccaataagga acaaacatgc atcatgaagt gctcagaaaa gttcttgaag
     7501 catagcgaac gtgtagggca gcgtttccaa gaacaaaacg ctgccttggg acaaggcttg
     7561 ggccgataag gtgtactggc gtatatatat ctaattatgt atctctggtg tagcccattt
     7621 ttagcatgta aatataaaga ccgagcggcc gcgtgttaca accaattaac caattctgat
     7681 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     7741 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     7801 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     7861 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     7921 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     7981 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     8041 gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     8101 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     8161 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     8221 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     8281 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     8341 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     8401 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     8461 ctggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     8521 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     8581 agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat
     8641 cagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8701 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8761 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8821 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8881 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8941 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9001 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9061 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9121 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9181 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9241 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9301 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9361 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg gccgccaatg
     9421 ctaaattcga gtgaaacaca ggaagatcag aaaatcctca tttcatccat attaacaata
     9481 atttcaaatg tttatttgca ttatttgaaa ctaggcaaga caagcaacga aacgtttttg
     9541 aaaattttga gtattttcaa taaatttgta gaggactcag atattgaaaa aaagctacag
     9601 caattaatac ttgataagaa gagtattgag aagggcaacg gttcatcatc tcatggatct
     9661 gcacatgaac aaacaccaga gtcaaacgac gttgaaattg aggctactgc gccaattgat
     9721 gacaatacag acgatgataa caaaccgaag ttatctgatg tagaaaagga ttaaagatgc
     9781 taagagatag tgatgatatt tcataaataa tgtaattcta tatatgttaa ttaccttttt
     9841 tgcgaggcat atttatggtg aaggataagt tttgaccatc aaagaaggtt aatgtggctg
     9901 tggtttcagg gtccataaag cccacatgga taacattacc cctgaattcg catctagact
     9961 gaactggccg ataattgcag acgaacgcct tgccaacagg gagttcttca gagacatgga
    10021 ggctcaaaac gaaattattg acagcctaga catcaatagt catacaacag aaagcgacca
    10081 cccaactttg gctgataata gcgtataaac aatgcatact ttgtacgttc aaaatacaat
    10141 gcagtagata tatttatgca tattacatat aatacatatc acataggaag caacaggcgc
    10201 gttggacttt taattttcga ggaccgcgaa tccttacatc acacccaatc ccccacaagt
    10261 gatcccccac acaccatagc ttcaaaatgt ttctactcct tttttactct tccagatttt
    10321 ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca agcacagcat actaaatttc
    10381 ccctctttct tcctctaggg tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa
    10441 agacaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt
    10501 ctttttcttg aaaatttttt tttttgattt ttttctcttt cgatgacctc ccattgatat
    10561 ttaagttaat aaacggtcat caatttctca agtttcagtt tcatttttct tgttctatta
    10621 caactttttt tacttcttgc tcattagaaa gaaagcatag caatctaatc taagttttaa
    10681 ttacaaaaga tctatggata aagcggaatt aattcccgag cctccaaaaa agaagagaaa
    10741 ggtcgaattg ggtaccgcc
//
