LOCUS       sequence-261259-        7087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains a reporter gene for Crz1. The 1000bp promoter of 
            the gene pCMK2 driving a Venus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Chen SY, Osimiri LC, Chevalier M, Bugaj LJ, Nguyen TH, Greenstein 
            RA, Ng AH, Stewart-Ornstein J, Neves LT, El-Samad H
  TITLE     Optogenetic Control Reveals Differential Promoter Interpretation of 
            Transcription Factor Nuclear Translocation Dynamics.
  JOURNAL   Cell Syst. 2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009.
  PUBMED    32898473
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009."
COMMENT     Sequence Label: pCMK2-Venus
FEATURES             Location/Qualifiers
     source          1..7087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1109..1822
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1838..2025
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1893..1917)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4627..5442)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4627..5442)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5350..5369
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5434..5453
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5667..6255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6156..6175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6605..7012
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 atctgaatca tgcgcggatg acacgaactc acgacggcga tcacagacat taacccacag
       61 tacagacact gcgacaacgt ggcaattcgt cgcaatacaa cgtgatgctt tcgaagcctg
      121 gtaatctaga gtattcaatg aacatgattg aaaaagcact aggccaggtg attataccgc
      181 agacttacgg taatataagg gcaagagaac taaaaaaatc aataatgtag gcttatatta
      241 cagttctttg tgagatattc ctttctagag cgttttctgt gtccactcaa ttaatatata
      301 cacttcccgt atcctaacat ttactgacac aatgataggc acaacgcgcg aaggagcggt
      361 ggggctttga acaataagga cccttacata gaaaaggcgt tagaacaaca atatggttag
      421 gccatagaaa cctcggtgtc ttttaggaac ttcacagaat agaacatact gcagtagata
      481 taaatacacc actggggcaa tgcagcctgg ttttttccaa atatacaaaa cacttcatga
      541 tacttactaa tggtcgttga tacagtactc attgtatcat tgcaggagag ttaggttggc
      601 ttttttgcat ttatttattt attcatttac tcaattgaca ggcaatgtca atgatttttt
      661 ggaagaaatt tgcctaatgt ttttactcag ttactcaccg ctccctggat aacataaggt
      721 tcatttgctt ccgtactgat tttttttggc agtctataag ccactatgta cttcccaatg
      781 ctcctgcagg catattcttt ttcaattttc atgtttttac ttccagtcgt tgagcatcat
      841 atggattcta tgttgatgag gcttttcaga acgtgctgta ctttcggaga agcgcccgct
      901 ttaacgcagc gcgaagagaa taattgaggc ttatcttaga accctgcccc ctttgaagcc
      961 acccattgct gtttctcttg gtaatcctaa taatataaaa caatacgata ttgttcaaga
     1021 tcagcagaac ttcaattcgt tgatcaatct tttcgtcacc ttttcttcta tcacatcgcc
     1081 aatataaata tagacaccaa aaagatctat gtctaaaggt gaagaattat tcactggtgt
     1141 tgtcccaatt ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg
     1201 tgaaggtgaa ggtgatgcta cttacggtaa attgacctta aaattgattt gtactactgg
     1261 taaattgcca gttccatggc caaccttagt cactacttta ggttatggtt tgcaatgttt
     1321 tgctagatac ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg
     1381 ttatgttcaa gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga
     1441 agtcaagttt gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa
     1501 agaaggtggt aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta
     1561 catcactgct gacaaacaaa agaatggtat caaagctaac ttcaaaatta gacacaacat
     1621 tgaagatggt ggtgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg
     1681 tccagtcttg ttaccagaca accattactt atcctatcaa tctgccttat ccaaagatcc
     1741 aaacgaaaag agagatcaca tggtcttgtt agaatttgtt actgctgctg gtattaccca
     1801 tggtatggat gaattgtaca aaggatccta actcgaggcg aatttcttat gatttatgat
     1861 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     1921 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     1981 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg
     2041 cctgcaaatc gctccccatt tcgctggaaa tctgctcgtc agtggtgctc acactgacga
     2101 atcatgtaca gatcataccg atgactgcct ggcgactcac aactaagcaa gacagccgga
     2161 accagcgccg gcgaacacca ctgcatatat ggcatatcac aacagtccac gtctcaagca
     2221 gttacagaga tgttacgaac cactagtgca ctgcagtaca ttaccaatag atagcggttt
     2281 aatataaggt ttcaacaaga aatattttgt gaaaatcaaa agttaatagt ttcactgtcg
     2341 aaaatatgaa agtttaagtt ttcagttcat cccattgtag ttatcaaatt tctgtgtttc
     2401 ccgtatatgt gtgctctgta ttcatcgcta tcttgaaact ggttatgatc gttgcgcatg
     2461 tgacctgtat cgctcaaaag agcccggcac cggtaccggg caaaagcccc tagcaagcta
     2521 aaatgattca actttcgtag taatttgttc aaccggtcac agtaaagtca atgactaact
     2581 tttgaatcga ggatgtattt ccaattgaaa gcaatgtata tataaaccat tgtgttgaag
     2641 cttgtcttac agttttcttg tatcctccca ttgttcatat agtacttaaa gtaccataat
     2701 ccttaaagaa caaacttgaa agagaataca ctatacacaa ctaataaata aagaataatc
     2761 atggctgtga ccaagacaat tgtagttcta ccaggtgacc atgttggtca agaaatcact
     2821 gaagaggcca ttaaagtttt aaatgctatt caggaatgtc gtccagacaa ggtcaatttc
     2881 aagtttgatc atcatttgat cggtggtgct gcaattgatg ccactggtgt tccattacca
     2941 gacgaagctt tggaggcctc taagaaagct gatgccgtgc ttctgggtgc tgttggtggt
     3001 ccaaaatggg gtactggcgc tgtcagacca gaacaaggtc ttttgaaaat ccgtaaggag
     3061 ttgcaattgt atgctaatct aagaccatgt aattttgcat ctgattcctt actagatcta
     3121 tcgccattga agcctgaaat tgcaagaggt acagatttcg tcgttgttag agaactagtg
     3181 ggtggtattt atttcgggga gagaaaagaa gatgaaggtg atggtgtcgc ctgggatagc
     3241 gaaaagtatt ctgtgcccga agttcaaaga atcactagaa tggctgcttt catggctctg
     3301 caacacaacc cacctttacc tatttggact ctagataaag ccaacgttct agcatcatca
     3361 agattatgga gaaaaacagt ggaaaagacg attaagaatg aattcccaca attgactgtt
     3421 caacatcaat tgattgattc cgctgctatg atcttggtta agaacccaac tcatctaaat
     3481 ggtattatta tcacaaacaa tatgtttggt gatattatct ccgatgaagc ctccgtgatc
     3541 ccaggttcgt taggattact tccatctgcc tccttggcat ctcttccaga taagaatacg
     3601 gcatttggtt tatatgagcc atgtcatggc tctgctccag acttaccaaa gggaaaggtt
     3661 aaccccgtag caacaatcct atctgctgct atgatgttga agttgtctct ggacctgttc
     3721 gaagaaggtg aaattataga acaagcagtc aagaaagtgt tggattctgg tatcagaaca
     3781 gcggatctaa agggtaccaa ctctactact gaggtcggtg atgcagtagc gaaagctgtc
     3841 agggaactat tagcttagac ttagtagatg tacagttgca tacataagtc tgaacagaaa
     3901 gaagtaaata tcatatctag ttcggtttag aaatcagtga ctatattggt tttgaccaga
     3961 tcgcaatcct tgcaagcatt tagtaatgac tatcaaaccg gtgaatcatc tgataaggct
     4021 atattagctt gtgcattcgc atgtatcggg aaacgaacga gtccaccgaa gtcggtgatg
     4081 ctgtcgccga agaagttaag aaaatccttg cttaaaaaga ttctcttttt ttatgatatt
     4141 tgtacataaa ctttataaat gaaattcata atagaaacga cacgaaatta caaaatggaa
     4201 tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga
     4261 aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa
     4321 ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt
     4381 taggtgtaac agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc
     4441 taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt
     4501 taagtcaata cctccttgaa ccatttccca taatggtgaa agttccctca agaattttac
     4561 tctgtcagaa acggccttaa cgacgtaccg agcggccgcg tgttacaacc aattaaccaa
     4621 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     4681 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     4741 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     4801 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     4861 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
     4921 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     4981 tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     5041 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     5101 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     5161 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     5221 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     5281 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     5341 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     5401 tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     5461 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     5521 acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt
     5581 gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5641 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5701 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5761 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5821 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5881 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5941 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6001 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6061 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc
     6361 gccaatgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
     6421 gaatcccaac gattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
     6481 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
     6541 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
     6601 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
     6661 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
     6721 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
     6781 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
     6841 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
     6901 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
     6961 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taccctgaat
     7021 tcgcatctag actgatgaga cgtggtagag ccacaaacag ccggtacaag caacgatctc
     7081 caggacc
//
