LOCUS       sequence-261267-        7086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains a reporter gene for Crz1. The 1000bp promoter of 
            the gene pGYP7 driving a Venus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Chen SY, Osimiri LC, Chevalier M, Bugaj LJ, Nguyen TH, Greenstein 
            RA, Ng AH, Stewart-Ornstein J, Neves LT, El-Samad H
  TITLE     Optogenetic Control Reveals Differential Promoter Interpretation of 
            Transcription Factor Nuclear Translocation Dynamics.
  JOURNAL   Cell Syst. 2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009.
  PUBMED    32898473
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009."
COMMENT     Sequence Label: pGYP7-Venus
FEATURES             Location/Qualifiers
     source          1..7086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1039..1752
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1768..1955
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1823..1847)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4557..5372)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4557..5372)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5280..5299
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5364..5383
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5597..6185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6086..6105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6535..6942
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 tgcgacaacg tggcaattcg tcgcaataca acgttgtaaa accttaactt ccattctaac
       61 gatgccaaat ctgcctaatg tagagcaaat aactgggaac actgggatga taaattcgaa
      121 ttctctataa tctgtcatag actttttact ataccttata actgcatcag gtgtaaaaat
      181 atctgatatt aattctgtcc agaatgaaag caggccaatc ttacccattg ccgtattcac
      241 ctcatttatt atttcgtaca atttcaacat cgccaaatta gataagtatt ttcttacatg
      301 atacggcacc cgtaacatag ataaagatgc ctgggatgca gaccgtggta acggagcagc
      361 tgccgttgct gctgtagcca tgggatgagg aaaggatgct accggcgtaa acattggcat
      421 agacccgctg ccagggcccc cagttgcatt cgtattctgc gcagagttgt gaaaagtttg
      481 agtcacttgt ggaagctggt taaacacctg ctgttctgat gaaggctgtg gaaattgacc
      541 cccctgtggt tgacctggaa atggcacttg ttgtggataa accgtgggcg gtatattctg
      601 ctgcgcggtg ggaggaaagt taatattacc atttgggtac ggtggtgtgt tattattcac
      661 aatgtaaggc atcggcacag cttgaggagg cggttgcggt gggccttggt acatattgca
      721 gataatcttt gcttttcctt ctgccccatg aacagtgtca agtgttgtct ttattgctcc
      781 atagttaata tttttagttc aggcgggaag ccccacaaga tgagaaaaga aatccgagag
      841 gcttaaacaa tactatatgg ggcaaagttc tgctctatat aaagagaggc gcatttgcaa
      901 gaaatagtta agaactttcc ataaattggt cagatataaa tcaatttatc gtgctcaaat
      961 atactattca gtttctcaga tataggttgc acaaattggc aaagttctac aagagtcatt
     1021 catacatccc ctagatctat gtctaaaggt gaagaattat tcactggtgt tgtcccaatt
     1081 ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa
     1141 ggtgatgcta cttacggtaa attgacctta aaattgattt gtactactgg taaattgcca
     1201 gttccatggc caaccttagt cactacttta ggttatggtt tgcaatgttt tgctagatac
     1261 ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg ttatgttcaa
     1321 gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga agtcaagttt
     1381 gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa agaaggtggt
     1441 aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta catcactgct
     1501 gacaaacaaa agaatggtat caaagctaac ttcaaaatta gacacaacat tgaagatggt
     1561 ggtgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg tccagtcttg
     1621 ttaccagaca accattactt atcctatcaa tctgccttat ccaaagatcc aaacgaaaag
     1681 agagatcaca tggtcttgtt agaatttgtt actgctgctg gtattaccca tggtatggat
     1741 gaattgtaca aaggatccta actcgaggcg aatttcttat gatttatgat ttttattatt
     1801 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     1861 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     1921 catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg cctgcaaatc
     1981 gctccccatt tcgctggaaa tctgctcgtc agtggtgctc acactgacga atcatgtaca
     2041 gatcataccg atgactgcct ggcgactcac aactaagcaa gacagccgga accagcgccg
     2101 gcgaacacca ctgcatatat ggcatatcac aacagtccac gtctcaagca gttacagaga
     2161 tgttacgaac cactagtgca ctgcagtaca ttaccaatag atagcggttt aatataaggt
     2221 ttcaacaaga aatattttgt gaaaatcaaa agttaatagt ttcactgtcg aaaatatgaa
     2281 agtttaagtt ttcagttcat cccattgtag ttatcaaatt tctgtgtttc ccgtatatgt
     2341 gtgctctgta ttcatcgcta tcttgaaact ggttatgatc gttgcgcatg tgacctgtat
     2401 cgctcaaaag agcccggcac cggtaccggg caaaagcccc tagcaagcta aaatgattca
     2461 actttcgtag taatttgttc aaccggtcac agtaaagtca atgactaact tttgaatcga
     2521 ggatgtattt ccaattgaaa gcaatgtata tataaaccat tgtgttgaag cttgtcttac
     2581 agttttcttg tatcctccca ttgttcatat agtacttaaa gtaccataat ccttaaagaa
     2641 caaacttgaa agagaataca ctatacacaa ctaataaata aagaataatc atggctgtga
     2701 ccaagacaat tgtagttcta ccaggtgacc atgttggtca agaaatcact gaagaggcca
     2761 ttaaagtttt aaatgctatt caggaatgtc gtccagacaa ggtcaatttc aagtttgatc
     2821 atcatttgat cggtggtgct gcaattgatg ccactggtgt tccattacca gacgaagctt
     2881 tggaggcctc taagaaagct gatgccgtgc ttctgggtgc tgttggtggt ccaaaatggg
     2941 gtactggcgc tgtcagacca gaacaaggtc ttttgaaaat ccgtaaggag ttgcaattgt
     3001 atgctaatct aagaccatgt aattttgcat ctgattcctt actagatcta tcgccattga
     3061 agcctgaaat tgcaagaggt acagatttcg tcgttgttag agaactagtg ggtggtattt
     3121 atttcgggga gagaaaagaa gatgaaggtg atggtgtcgc ctgggatagc gaaaagtatt
     3181 ctgtgcccga agttcaaaga atcactagaa tggctgcttt catggctctg caacacaacc
     3241 cacctttacc tatttggact ctagataaag ccaacgttct agcatcatca agattatgga
     3301 gaaaaacagt ggaaaagacg attaagaatg aattcccaca attgactgtt caacatcaat
     3361 tgattgattc cgctgctatg atcttggtta agaacccaac tcatctaaat ggtattatta
     3421 tcacaaacaa tatgtttggt gatattatct ccgatgaagc ctccgtgatc ccaggttcgt
     3481 taggattact tccatctgcc tccttggcat ctcttccaga taagaatacg gcatttggtt
     3541 tatatgagcc atgtcatggc tctgctccag acttaccaaa gggaaaggtt aaccccgtag
     3601 caacaatcct atctgctgct atgatgttga agttgtctct ggacctgttc gaagaaggtg
     3661 aaattataga acaagcagtc aagaaagtgt tggattctgg tatcagaaca gcggatctaa
     3721 agggtaccaa ctctactact gaggtcggtg atgcagtagc gaaagctgtc agggaactat
     3781 tagcttagac ttagtagatg tacagttgca tacataagtc tgaacagaaa gaagtaaata
     3841 tcatatctag ttcggtttag aaatcagtga ctatattggt tttgaccaga tcgcaatcct
     3901 tgcaagcatt tagtaatgac tatcaaaccg gtgaatcatc tgataaggct atattagctt
     3961 gtgcattcgc atgtatcggg aaacgaacga gtccaccgaa gtcggtgatg ctgtcgccga
     4021 agaagttaag aaaatccttg cttaaaaaga ttctcttttt ttatgatatt tgtacataaa
     4081 ctttataaat gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata
     4141 gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga
     4201 tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa
     4261 ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt taggtgtaac
     4321 agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc taaaaaaaaa
     4381 aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt taagtcaata
     4441 cctccttgaa ccatttccca taatggtgaa agttccctca agaattttac tctgtcagaa
     4501 acggccttaa cgacgtaccg agcggccgcg tgttacaacc aattaaccaa ttctgattag
     4561 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     4621 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     4681 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     4741 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     4801 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     4861 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     4921 tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     4981 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     5041 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     5101 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     5161 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     5221 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     5281 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg
     5341 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     5401 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     5461 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     5521 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6121 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6181 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6241 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc gccaatgagg
     6301 agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg gaatcccaac
     6361 gattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt
     6421 gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca agtaattggt
     6481 tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc agttaactgt
     6541 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt
     6601 tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg
     6661 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg
     6721 ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt
     6781 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg
     6841 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt
     6901 tcagcaatat atatatatat atttcaagga tataccattc taccctgaat tcgcatctag
     6961 actgatgaga cgtggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc
     7021 tgaatcatgc gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac
     7081 agacac
//
