LOCUS       sequence-261283-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MUS81.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     493..512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     746..763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1003..1024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1039..1044,1045..1062,1063..1069)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1077..1093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1082..1104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1101..1117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1101..1117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1167..1186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1378..2011
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2058..2183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2680..2913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3098..3142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3435..3552
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3604..3907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3908..4111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4062..4082
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4113..4131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4134..4152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4162..4186
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4273..4282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4285..4311
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4327..4351
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6005..6029)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6048..6547
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6083..6106)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6480..6499
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6568..7167
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6568..6587)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7064..7084
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7234..7822
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7287..7307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7705..7716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7897..8130
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8249..8267)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8335..8439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8440..8508,8509..9300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8658..8677)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
       61 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      121 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      361 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      421 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      661 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      721 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      781 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
      841 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
      901 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
      961 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     1021 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     1081 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
     1141 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1201 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     1261 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     1321 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     1381 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     1441 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     1501 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     1561 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     1621 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     1681 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     1741 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     1801 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     1861 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     1921 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     1981 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     2041 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     2101 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     2161 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     2221 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     2281 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     2341 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     2401 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     2461 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     2521 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     2581 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     2641 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     2701 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     2761 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     2821 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     2881 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     2941 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     3001 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     3061 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     3121 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     3181 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     3241 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     3301 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     3361 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca
     3421 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     3481 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     3541 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac tagtggagtt
     3601 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     3661 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     3721 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     3781 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     3841 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     3901 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     3961 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     4021 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     4081 tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag atctccctat
     4141 cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     4201 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc
     4261 gtttaaacgc tagccaccat ggcgtaccca tacgatgttc cagattacgc tgctcgaggt
     4321 tatcaaacaa gtttgtacaa aaaagttggc atggcggccc cggtccgcct gggccggaag
     4381 cgcccgctgc ctgcctgtcc caacccgctc ttcgttcgct ggctgaccga gtggcgggac
     4441 gaggcgaccc gcagcaggca ccgcacgcgc ttcgtatttc agaaggcgct gcgttccctc
     4501 cgacggtacc cactgccgct gcgcagcggg aaggaagcta agatcctaca gcacttcgga
     4561 gacgggctct gccggatgct ggacgagcgg ctgcagcggc accgaacatc gggcggtgac
     4621 catgccccgg actcaccatc tggagagaac agtccagccc cgcaggggcg acttgcggaa
     4681 gtccaggact cttccatgcc agttcctgcc cagcccaaag cgggaggctc tggcagctac
     4741 tggccagctc ggcactcagg agcccgagtg atactgctgg tgctctaccg ggagcacctg
     4801 aatcctaatg gtcaccactt cttaaccaag gaggagctgc tgcagaggtg tgctcagaag
     4861 tcccccaggg tagcccctgg gagtgcccca ccctggccag ccctccgctc cctccttcac
     4921 aggaacctgg tcctcaggac acaccagcca gccaggtact cattgacccc agagggcctg
     4981 gagctggccc agaagttggc cgagtcagaa ggcctgagct tgctgaatgt gggcatcggg
     5041 cccaaggagc cccctgggga ggagacagca gtgccaggag cagcttcagc agagcttgcc
     5101 agtgaagcag gggtccagca gcagccactg gagctgaggc ctggagagta cagggtgctg
     5161 ttgtgtgtgg acattggcga gacccggggg ggcgggcaca ggccggagct gctccgagag
     5221 ctacagcggc tgcacgtgac ccacacggtg cgcaagctgc acgttggaga ttttgtgtgg
     5281 gtggctcagg agaccaatcc tagagaccca gcaaaccctg gggagttggt actggatcac
     5341 attgtggagc gcaagcgact ggatgacctt tgcagcagca tcatcgacgg ccgcttccgg
     5401 gagcagaagt tccgactgaa gcgctgtggt ctggagcgcc gggtatacct ggtggaagag
     5461 catggttccg tccacaacct cagccttcct gagagcacac tgctgcaggc tgtcaccaac
     5521 actcaggtca ttgatggctt ttttgtgaag cgcacagcag acattaagga gtcagccgcc
     5581 tacctggccc tcttgactcg gggcctgcag agactctacc agggccacac cctacgcagc
     5641 cgcccctggg gaacccctgg gaaccctgaa tcaggggcca tgacctctcc aaaccctctc
     5701 tgctcactcc tcaccttcag tgacttcaac gcaggagcca tcaagaataa ggcccagtcg
     5761 gtgcgagaag tgtttgcccg gcagctgatg caggtgcgcg gagtgagtgg ggagaaggca
     5821 gcagccctgg tggatcgata cagcacccct gccagcctcc tggccgccta tgatgcctgt
     5881 gccaccccca aggaacaaga gacactgctg agcaccatta agtgtgggcg tctacagagg
     5941 aatctggggc ctgctctgag caggacctta tcccagctct actgcagcta cggccccttg
     6001 acctgcccaa ctttcttgta caaagtggtt cgataacgaa ttctaccggg taggggaggc
     6061 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     6121 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     6181 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     6241 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6301 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6361 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6421 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6481 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6541 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     6601 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6661 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6721 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6781 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6841 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6901 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6961 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7021 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7081 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7141 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     7201 aggagcgcac gaccccatgc atcgatagat cctaatcaac ctctggatta caaaatttgt
     7261 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7321 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7381 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7441 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7501 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7561 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7621 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7681 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7741 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7801 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     7861 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7921 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8101 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     8161 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     8221 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     8281 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     8341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8401 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8461 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8521 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8581 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8641 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     8701 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8761 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8821 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8881 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8941 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9001 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9061 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9121 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9181 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9241 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9301 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9361 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatc
//
