LOCUS       sequence-26131-e         466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Wiesner SM, Decker SA, Larson JD, Ericson K, Forster C, Gallardo JL,
            Long C, Demorest ZL, Zamora EA, Low WC, SantaCruz K, Largaespada DA,
            Ohlfest JR
  TITLE     De novo induction of genetically engineered brain tumors in mice 
            using plasmid DNA.
  JOURNAL   Cancer Res. 2009 Jan 15. 69(2):431-9.
  PUBMED    19147555
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2009 Jan 15. 69(2):431-9."
COMMENT     Sequence Label: pT/Caggs-NRASV12
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(4..22)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5..22)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             56..73
                     /codon_start=1
                     /product="epitope tag from middle T antigen of mouse 
                     polyoma virus"
                     /label=Glu-Glu tag
                     /translation="EYMPME"
ORIGIN
        1 tattctatag tgtcacctaa atgctagagc tcgctgatcc accanggagg aagaggaata
       61 tatgcctatg gaaatgactg agtacaaact ggtggtggtt ggagcagttg gtgttgggaa
      121 aagcgcactg accatccagc taatccagaa ccactttgta gatgaatatg atcccaccat
      181 agaggattct tacagaaaac aagtggttat agatggtgaa acctgtttgt tggacatact
      241 ggatacagct ggacaagaag agtacagtgc catgagagac caatacatga ggacaggcga
      301 aggcttcctc tgtgtatttg ccatcaataa tagcaagtca tttgcggata ttaacctcta
      361 cagggaacag attaagcgag taaaagactc ggatgatgta cctatggtgc tagtgggaaa
      421 caagtgtgat ttgccaacaa ggacagttga tacaaaacaa gcccac
//
