LOCUS       sequence-261317-       10359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   De Iaco A, Luban J
  TITLE     Inhibition of HIV-1 infection by TNPO3 depletion is determined by 
            capsid and detectable after viral cDNA enters the nucleus.
  JOURNAL   Retrovirology. 2011 Dec 6;8:98. doi: 10.1186/1742-4690-8-98.
  PUBMED    22145813
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Retrovirology."; date: "2011-12-6"; volume: "8:98. doi"; pages: " 
            10.1186/1742-4690-8-98"
COMMENT     Sequence Label: p8.9 NdeltaSB D185K/D186L
FEATURES             Location/Qualifiers
     source          1..10359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             479..1981
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1774..4785
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMK
                     LLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4470..4587
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5359..5592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5777..5821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6115..6141
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        complement(6764..6782)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6765..6782)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7072..7091)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7305..7324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7424..7446
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        7570..7674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7675..7743,7744..8535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7893..7912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8706..9294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9195..9214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9448..9465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9535..9555)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9540..9869
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9720..9855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        10056..10359
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
      301 cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc ctatagagtc
      361 tataggccca cccccttggc ttcttatgcg acggatcgat cccgtaataa gcttcgaggt
      421 ccgcggccgg ccgcgttgac gcgcacggca agaggcgagg ggcggccgct ggtgagagat
      481 gggtgcgaga gcgtcggtat taagcggggg agaattagat aaatgggaaa aaattcggtt
      541 aaggccaggg ggaaagaaac aatataaact aaaacatata gtatgggcaa gcagggagct
      601 agaacgattc gcagttaatc ctggcctttt agagacatca gaaggctgta gacaaatact
      661 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
      721 aatagcagtc ctctattgtg tgcatcaaag gatagatgta aaagacacca aggaagcctt
      781 agataagata gaggaagagc aaaacaaaag taagaaaaag gcacagcaag cagcagctga
      841 cacaggaaac aacagccagg tcagccaaaa ttaccctata gtgcagaacc tccaggggca
      901 aatggtacat caggccatat cacctagaac tttaaatgca tgggtaaaag tagtagaaga
      961 gaaggctttc agcccagaag taatacccat gttttcagca ttatcagaag gagccacccc
     1021 acaagattta aataccatgc taaacacagt ggggggacat caagcagcca tgcaaatgtt
     1081 aaaagagacc atcaatgagg aagctgcaga atgggataga ttgcatccag tgcatgcagg
     1141 gcctattgca ccaggccaga tgagagaacc aaggggaagt gacatagcag gaactactag
     1201 tacccttcag gaacaaatag gatggatgac acataatcca cctatcccag taggagaaat
     1261 ctataaaaga tggataatcc tgggattaaa taaaatagta agaatgtata gccctaccag
     1321 cattctggac ataagacaag gaccaaagga accctttaga gactatgtag accgattcta
     1381 taaaactcta agagccgagc aagcttcaca agaggtaaaa aattggatga cagaaacctt
     1441 gttggtccaa aatgcgaacc cagattgtaa gactatttta aaagcattgg gaccaggagc
     1501 gacactagaa gaaatgatga cagcatgtca gggagtgggg ggacccggcc ataaagcaag
     1561 agttttggct gaagcaatga gccaagtaac aaatccagct accataatga tacagaaagg
     1621 caattttagg aaccaaagaa agactgttaa gtgtttcaat tgtggcaaag aagggcacat
     1681 agccaaaaat tgcagggccc ctaggaaaaa gggctgttgg aaatgtggaa aggaaggaca
     1741 ccaaatgaaa gattgtactg agagacaggc taatttttta gggaagatct ggccttccca
     1801 caagggaagg ccagggaatt ttcttcagag cagaccagag ccaacagccc caccagaaga
     1861 gagcttcagg tttggggaag agacaacaac tccctctcag aagcaggagc cgatagacaa
     1921 ggaactgtat cctttagctt ccctcagatc actctttggc agcgacccct cgtcacaata
     1981 aagatagggg ggcaattaaa ggaagctcta ttagatacag gagcagatga tacagtatta
     2041 gaagaaatga atttgccagg aagatggaaa ccaaaaatga tagggggaat tggaggtttt
     2101 atcaaagtaa gacagtatga tcagatactc atagaaatct gcggacataa agctataggt
     2161 acagtattag taggacctac acctgtcaac ataattggaa gaaatctgtt gactcagatt
     2221 ggctgcactt taaattttcc cattagtcct attgagactg taccagtaaa attaaagcca
     2281 ggaatggatg gcccaaaagt taaacaatgg ccattgacag aagaaaaaat aaaagcatta
     2341 gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt caaaaattgg gcctgaaaat
     2401 ccatacaata ctccagtatt tgccataaag aaaaaagaca gtactaaatg gagaaaatta
     2461 gtagatttca gagaacttaa taagagaact caagatttct gggaagttca attaggaata
     2521 ccacatcctg cagggttaaa acagaaaaaa tcagtaacag tactggatgt gggcgatgca
     2581 tatttttcag ttcccttaga taaagacttc aggaagtata ctgcatttac catacctagt
     2641 ataaacaatg agacaccagg gattagatat cagtacaatg tgcttccaca gggatggaaa
     2701 ggatcaccag caatattcca gtgtagcatg acaaaaatct tagagccttt tagaaaacaa
     2761 aatccagaca tagtcatcta tcaatacatg aagcttttgt atgtaggatc tgacttagaa
     2821 atagggcagc atagaacaaa aatagaggaa ctgagacaac atctgttgag gtggggattt
     2881 accacaccag acaaaaaaca tcagaaagaa cctccattcc tttggatggg ttatgaactc
     2941 catcctgata aatggacagt acagcctata gtgctgccag aaaaggacag ctggactgtc
     3001 aatgacatac agaaattagt gggaaaattg aattgggcaa gtcagattta tgcagggatt
     3061 aaagtaaggc aattatgtaa acttcttagg ggaaccaaag cactaacaga agtagtacca
     3121 ctaacagaag aagcagagct agaactggca gaaaacaggg agattctaaa agaaccggta
     3181 catggagtgt attatgaccc atcaaaagac ttaatagcag aaatacagaa gcaggggcaa
     3241 ggccaatgga catatcaaat ttatcaagag ccatttaaaa atctgaaaac aggaaagtat
     3301 gcaagaatga agggtgccca cactaatgat gtgaaacaat taacagaggc agtacaaaaa
     3361 atagccacag aaagcatagt aatatgggga aagactccta aatttaaatt acccatacaa
     3421 aaggaaacat gggaagcatg gtggacagag tattggcaag ccacctggat tcctgagtgg
     3481 gagtttgtca atacccctcc cttagtgaag ttatggtacc agttagagaa agaacccata
     3541 ataggagcag aaactttcta tgtagatggg gcagccaata gggaaactaa attaggaaaa
     3601 gcaggatatg taactgacag aggaagacaa aaagttgtcc ccctaacgga cacaacaaat
     3661 cagaagactg agttacaagc aattcatcta gctttgcagg attcgggatt agaagtaaac
     3721 atagtgacag actcacaata cgcgttggga atcattcaag cacaaccaga taagagtgaa
     3781 tcagagttag tcagtcaaat aatagagcag ttaataaaaa aggaaaaagt ctacctggca
     3841 tgggtaccag cacacaaagg aattggagga aatgaacaag tagataaatt ggtcagtgct
     3901 ggaatcagga aagtactatt tttagatgga atagataagg cccaagaaga acatgagaaa
     3961 tatcacagta attggagagc aatggctagt gattttaacc taccacctgt agtagcaaaa
     4021 gaaatagtag ccagctgtga taaatgtcag ctaaaagggg aagccatgca tggacaagta
     4081 gactgtagcc caggaatatg gcagctagat tgtacacatt tagaaggaaa agttatcttg
     4141 gtagcagttc atgtagccag tggatatata gaagcagaag taattccagc agagacaggg
     4201 caagaaacag catacttcct cttaaaatta gcaggaagat ggccagtaaa aacagtacat
     4261 acagacaatg gcagcaattt caccagtact acagttaagg ccgcctgttg gtgggcgggg
     4321 atcaagcagg aatttggcat tccctacaat ccccaaagtc aaggagtaat agaatctatg
     4381 aataaagaat taaagaaaat tataggacag gtaagagatc aggctgaaca tcttaagaca
     4441 gcagtacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     4501 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4561 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     4621 tggaaaggac cagcaaagct cctctggaaa ggtgaagggg cagtagtaat acaagataat
     4681 agtgacataa aagtagtgcc aagaagaaaa gcaaagatca tcagggatta tggaaaacag
     4741 atggcaggtg atgattgtgt ggcaagtaga caggatgagg attaacacat ggaattctgc
     4801 aacaactgct gtttatccat ttcagaattg ggtgtcgaca tagcagaata ggcgttactc
     4861 gacagaggag agcaagaaat ggagccagta gatcctagac tagagccctg gaagcatcca
     4921 ggaagtcagc ctaaaactgc ttgtaccaat tgctattgta aaaagtgttg ctttcattgc
     4981 caagtttgtt tcatgacaaa agccttaggc atctcctatg gcaggaagaa gcggagacag
     5041 cgacgaagag ctcatcagaa cagtcagact catcaagctt ctctatcaaa gcagtaagta
     5101 gtacatgtaa tgcaacctat aatagtagca atagtagcat tagtagtagc aataataata
     5161 gcaatagttg tgtggtccat agtaatcata gaatatagga aaatggccgc tgatcttcag
     5221 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     5281 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     5341 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     5401 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     5461 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     5521 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     5581 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     5641 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     5701 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     5761 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     5821 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     5881 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     5941 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     6001 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     6061 gaacggatcc ttggcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca
     6121 ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg
     6181 gtgggaagcc ctcaaatatt ggtggaatct cctacaatat tggagtcagg agctaaagaa
     6241 tagtgctgtt agcttgctca atgccacagc catagcagta gctgagggga cagatagggt
     6301 tatagaagta gtacaaggag cttgtagagc tattcgccac atacctagaa gaataagaca
     6361 gggcttggaa aggattttgc tataagctcg aggccgcccc ggtgaccttc agaccttggc
     6421 actggaggtg gcccggcaga agcgcggcat cgtggatcag tgctgcacca gcatctgctc
     6481 tctctaccaa ctggagaact actgcaacta ggcccaccac taccctgtcc acccctctgc
     6541 aatgaataaa acctttgaaa gagcactaca agttgtgtgt acatgcgtgc atgtgcatat
     6601 gtggtgcggg gggaacatga gtggggctgg ctggagtggc gatgataagc tgtcaaacat
     6661 gagaattaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6721 catgataata atggtttctt agtctagaat taattccgtg tattctatag tgtcacctaa
     6781 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata
     6841 tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt
     6901 aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc
     6961 cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg
     7021 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     7081 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     7141 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     7201 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     7261 tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat
     7321 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7381 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7441 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     7501 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7561 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7621 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7681 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7741 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7801 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7861 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7921 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7981 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8041 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8101 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8161 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8221 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8281 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8341 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8401 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8461 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8521 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8581 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8641 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8821 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9061 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9301 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9361 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9421 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9481 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg
     9541 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9601 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9661 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9721 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9781 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     9841 gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata
     9901 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     9961 tcatgtgaaa tactggtttt tagtgcgcca gatcctagtt attaatagta atcaattacg
    10021 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
    10081 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
    10141 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
    10201 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
    10261 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
    10321 tggcagtaca tctacgtatt agtcatcgct attaccatg
//
