LOCUS       sequence-26134-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Wiesner SM, Decker SA, Larson JD, Ericson K, Forster C, Gallardo JL,
            Long C, Demorest ZL, Zamora EA, Low WC, SantaCruz K, Largaespada DA,
            Ohlfest JR
  TITLE     De novo induction of genetically engineered brain tumors in mice 
            using plasmid DNA.
  JOURNAL   Cancer Res. 2009 Jan 15. 69(2):431-9.
  PUBMED    19147555
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2009 Jan 15. 69(2):431-9."
COMMENT     Sequence Label: pT2/shp53/GFP4
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(29..358)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      43..178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     343..363
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     ncRNA           complement(396..522)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(550..564)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(592..713)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gctcctcgcc cttgctcacc atgagctttt tgcaaaagcc taggcctcca aaaaagcctc
       61 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
      121 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
      181 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
      241 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
      301 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
      361 tccacagggt cgaccactgt gctggcgaat tgaaaaaagt gatttaattt ataccatttt
      421 aattcagctt tgtaaaaatg tatcaaagag atagcaaggt attcagtttt agtaaacaag
      481 ataattgctc ctaaagtagc cccttgaatt ccgaggcagt aggcatccac tacaagtaca
      541 tgtgtaatac atctgtggct tcactattac acatgtactt gtagtgggcg ctcactgtca
      601 acagcaatat accttctcga gccttctgtt gggttaacct gaagaagtaa tcccagcaag
      661 tgtttccaag atgtgcaggc aacgattctg taaagtactg aagcctcatt caaacaatta
      721 ccctgttatc
//
