LOCUS       sequence-261344-       11425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen
            TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, 
            El-Samad H, Baker D
  TITLE     De novo design of bioactive protein switches.
  JOURNAL   Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 
            10.1038/s41586-019-1432-8.
  PUBMED    31341284
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 
            10.1038/s41586-019-1432-8."
COMMENT     Sequence Label: pAN2981
FEATURES             Location/Qualifiers
     source          1..11425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..504
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(40..63)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     437..456
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             685..1380
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             1387..1443
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1459..2166
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1604..1622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1872..1891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3030..3618
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3083..3103)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3501..3512)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3633..3649)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3634..3650)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3740..3973
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3998..4016)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3999..4016)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4306..4325)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4539..4558)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4658..4680
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4718..4736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(4915..5503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4995..5014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(5717..5736)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5728..6543
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             5728..6543
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5801..5820)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6718..6735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6805..6825)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6810..7139
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6990..7125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8273..8338
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8468..8488
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8913..9047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8950..8969)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9004..9023
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9215..9848
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9895..10020
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10517..10750
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10935..10979
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11264..11381
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aacggggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
       61 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
      121 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
      181 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
      241 gcatgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
      301 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
      361 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
      421 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
      481 tcttcctcat ctccgggcct ttcggccact atggaggcca ggaaagcgat tgcccgagtt
      541 aaaagggaat ctaagagaat tgtagaagat gcggaaaggt tgatacgcga agccgccgct
      601 gcttcagaaa aaatctcacg agaggcggaa cgactcatcc gagaggcggc tgccgcttct
      661 gaaaaaatat ccagggaggg ttctagcgag ctgattaagg agaacatgca catgaagctg
      721 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
      781 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
      841 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
      901 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
      961 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     1021 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     1081 cagaagaaaa cactcggctg ggaggccttc accgaaacgc tgtaccccgc tgacggcggc
     1141 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     1201 atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     1261 tatgtggact acagactgga aagaatcaag gaggccaaca acgaaaccta cgtcgagcag
     1321 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     1381 ggatccgcca ctaacttctc tctgctgaaa caagccgggg acgtggagga gaatccaggt
     1441 cccgctgcca aaacgggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     1501 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1561 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1621 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1681 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1741 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1801 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1861 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1921 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1981 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2041 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2101 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     2161 tacaagggat cctctgagtt ggctagaaaa ttgttagagg catctaccaa gttacagaga
     2221 ttgaatatta ggttggctga agctttattg gaagctatag ctagattgca agagttaaat
     2281 ttggaattag tttacttggc cgttgaatta accgatccta aaaggataag ggatgaaata
     2341 aaggaggtta aagataagtc taaagagatc attaggagag ctgagaagga aattgatgat
     2401 gctgccaaag aatcagaaaa gattttagaa gaagccaggg aggctatctc aggttcaggt
     2461 tcagaattgg ccaaattatt attaaaagca attgcagaaa ctcaagattt gaacttaaga
     2521 gcagcaaagg ccttcttgga ggccgcagcc aaacttcaag aactaaacat tagagctgtc
     2581 gaattgttgg taaagttgac tgatccagct acaatcagag aggcattaga acatgctaag
     2641 agaagatcaa aggaaatcat agacgaggcc gaaagagcaa taagggccgc taaaagagag
     2701 tctgaacgta tcatcgaaga agctcgtaga ttaattgaaa agggcagcgg ttccggatct
     2761 gaattagcaa gagaattgtt gagggctcat gcccagttgc aaagattaaa tttggaacta
     2821 ttacgtgagt tgttaagagc cttagcacag ttacaggagc ttaatctgga tttattaagg
     2881 ttggcctcag agttaacaga cccagatgag gccaggaaag ctatcgcccg tgtaaaacgt
     2941 ttatcaatga gctgcgctca agaatcagag aggttgatca gagaagcggc tgctgcatca
     3001 gagaagatta agtaatggcg cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3061 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3121 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3181 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3241 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3301 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3361 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3421 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3481 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3541 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3601 tttgggccgc ctccccgcat gctgagcata cacgataccg tcgacctcga gggaattaat
     3661 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3721 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3781 tttgcttgta ctgggtttct ctggttagac cagatctgag cctgggagct ctctggctaa
     3841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3961 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
     4021 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
     4081 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
     4141 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
     4201 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
     4261 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     4321 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     4381 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     4441 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     4501 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
     4561 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4621 ctcccggcat ccgcttacag acaagctgtg accatctccg ggagctgcat gtgtcagagg
     4681 ttttcaccgt catcaccgaa acgcgcgaga tgaaagggcc tcgtgatacg cctattttta
     4741 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4801 gtgccgagcg gccgcacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5101 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5341 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgac
     5581 tgatccttca actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat
     5641 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     5701 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcc
     5761 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     5821 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
     5881 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     5941 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     6001 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa
     6061 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     6121 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg cctcgctcag
     6181 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     6241 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
     6301 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     6361 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     6421 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     6481 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     6541 taatcagaat tggttaattg gttgtaacac gcggccgcca attttttacg gttcctggcc
     6601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6781 tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6901 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6961 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7021 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7081 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7141 agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca
     7201 gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca
     7261 gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa
     7321 caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc
     7381 catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc
     7441 gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg
     7501 tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga
     7561 aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg
     7621 gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa
     7681 aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga
     7741 ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt
     7801 ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt
     7861 ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc
     7921 aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca
     7981 caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt
     8041 aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca
     8101 cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc
     8161 taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt
     8221 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     8281 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     8341 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     8401 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     8461 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     8521 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     8581 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     8641 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     8701 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     8761 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     8821 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     8881 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     8941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     9061 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     9121 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     9181 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     9241 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     9301 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     9361 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     9421 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     9481 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     9541 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     9601 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     9661 ctgtactggg actctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     9901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     9961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    10021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    10081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    10141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    10201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    10261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    10321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
    10381 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
    10441 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
    10501 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
    10561 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
    10621 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    10681 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    10741 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    10801 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    10861 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10921 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10981 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    11041 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    11101 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    11161 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
    11221 acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg
    11281 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    11341 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    11401 gcagagatcc agtttggccc tctga
//
