LOCUS       sequence-26137-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Boothroyd CE, Dreesen O, Leonova T, Ly KI, Figueiredo LM, Cross GA, 
            Papavasiliou FN
  TITLE     A yeast-endonuclease-generated DNA break induces antigenic switching
            in Trypanosoma brucei.
  JOURNAL   Nature. 2009 May 14;459(7244):278-81. doi: 10.1038/nature07982. Epub
            2009 Apr 15.
  PUBMED    19369939
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07982"; journalName: "Nature"; date: "2009-05-14- 
            14"; volume: "459"; issue: "7244"; pages: "278-81"
COMMENT     Sequence Label: pLew100::NLS-ISceI-HA
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(684..704)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    834..852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    855..873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agtgttcggc agttgtacat catctgcggg atcaggtacg gtttgatcag gttgtagaag
       61 atcaggtaag acatagaatc gatgtagatg atcggtttgt ttttgttgat ttttacgtaa
      121 cagttcagtt ggaatttgtt acgcagaccc ttaaccaggt attctacttc ttcgaaagtg
      181 aaagactggg tgttcagtac gatcgatttg ttggtagagt ttttgttgta atcccattta
      241 ccaccatcat ccatgaacca gtatgccaga gacatcgggg tcaggtagtt ttcaaccagg
      301 ttgttcggga tggttttttt gttgttaacg atgaacaggt tagccagttt gttgaatgct
      361 tggtgtttga aagtctgggc gccccaggtg attaccaggt tacccaggtg gttaacacgt
      421 tcttttttgt gcggcgggga cagtacccac tgatcgtaca gcagacatac gtggtccatg
      481 tatgctttgt ttttccactc gaactgcata cagtaggttt taccttcatc acgagaacgg
      541 atgtaagcat cacccaggat cagaccgata cctgcttcga actgttcgat gttcagttcg
      601 atcagctggg atttgtattc tttcagcagt ttagagttcg gacccaggtt cattacctgg
      661 ttttttttga tgtttttcat atgtaccttt cgcttcttct ttggcataag cttgtgaatt
      721 ttactttttg gtgtaattga agtcttcaag ttgaaaaaaa tggggaaaag gaaaaaaaac
      781 tcgaaggcgc gtgcagcccg ggacgagaac atgttaaact cagtactcag ggatctctat
      841 cactgatagg gagatctcta tcactgatag ggagatccct acccgataac ccctattttc
      901 aatgcacg
//
