LOCUS       sequence-261371-        2481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes permuteposon P1 for MuA transposase-mediated circular 
            permutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Atkinson JT, Wu B, Segatori L, Silberg JJ
  TITLE     Overcoming component limitations in synthetic biology through 
            transposon-mediated protein engineering.
  JOURNAL   Methods Enzymol. 2019;621:191-212. doi: 10.1016/bs.mie.2019.02.014. 
            Epub 2019 Apr 2.
  PUBMED    31128779
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2019"; volume: "621:191-212. doi"; pages: " 
            10.1016/bs.mie.2019.02.014. Epub 2019 Apr 2"
COMMENT     Sequence Label: pMT-P1
FEATURES             Location/Qualifiers
     source          1..2481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..88)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     RBS             809..817
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     rep_origin      complement(1767..2355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1847..1866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(join(2466..2481,1..4))
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(2477..2481,1..811)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALTVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga
       61 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
      121 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
      181 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
      241 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
      301 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
      361 tgcgctgaca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
      421 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
      481 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
      541 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
      601 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
      661 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
      721 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
      781 gtttcatttg atgctcgatg agtttttcta aggaggtcag ctatgggttt tcgcatttat
      841 cgtgaaacgc tttcgcgttt ttcgtgcgcc gctcaagatc taatgatggc ttatctggtg
      901 tttctcggac ctccaggggc cggaaaaggt acgtacgcaa agcgaatcca agaaaagacg
      961 ggtattcccc atatatccac cggcgacatc ttcagagata ttgtgaagaa agaaaacgat
     1021 gaacttggaa agaaaataaa ggagataatg gaaaagggag aactggttcc tgatgaactg
     1081 gtgaacgagg ttgtgaaaag aagactttca gagaaagatt gtgaaaaggg gttcattctg
     1141 gatgggtatc caaggaccgt agcccaggcg gagtttctcg actctttcct ggaatcccaa
     1201 aacaagcaac tcacagctgc cgtgcttttt gatgttccag aggacgtggt tgtccagagg
     1261 ctcacctcga gaagaatctg cccaaagtgc ggtagaatct acaacgtgat ttcacttcaa
     1321 ccaaaagaag acgaactgtg cgatgactgt aaagtaaaac tcgttcagcg agacgacgac
     1381 aaagaggaaa cggtcagaca cagatacaag gtttacctcg aaaaaactca acccgtgata
     1441 gactattacg ggaaaaaggg tatcctcaaa agagtggacg gcacgatagg gatcgacaac
     1501 gtggttgccg aggtactcaa aataataggg tggagtgata aatgagatct tgagcggcgc
     1561 acgaaaaacg cgaaagcgtt tcacgataaa tgcgaaaaaa aggatctcaa gaagatcctt
     1621 tgattttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1681 agaatcaggg gataacgcag gaaagaacat gtgagcaaag gccagcaaaa ggccaggaac
     1741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2401 gaaaactcac gttaagggat tttggtcatg aacaataaaa ctgtctgctt acataaacag
     2461 taatacaagg ggtgttatga g
//
