LOCUS       sequence-261374-        5111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adeno-Associated viral (AAV) vector plasmid expressing exon1 of 
            mutant HTT (mHTT, 66 polyQ repeats) in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Pircs K, Petri R, Madsen S, Brattas PL, Vuono R, Ottosson DR, 
            St-Amour I, Hersbach BA, Matusiak-Bruckner M, Lundh SH, Petersen A, 
            Deglon N, Hebert SS, Parmar M, Barker RA, Jakobsson J
  TITLE     Huntingtin Aggregation Impairs Autophagy, Leading to Argonaute-2 
            Accumulation and Global MicroRNA Dysregulation.
  JOURNAL   Cell Rep. 2018 Aug 7;24(6):1397-1406. doi: 
            10.1016/j.celrep.2018.07.017.
  PUBMED    30089251
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.07"; journalName: "Cell Rep"; date: 
            "2018-08-7- 7"; volume: "24"; issue: "6"; pages: "1397-1406"
COMMENT     Sequence Label: pAAV-hSyn-Htt171-66Q-myc-WPRE
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   34..163
                     /label=AAV2 ITR
     promoter        223..670
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             1364..1393
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1409..1426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    1448..2036
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1501..1521)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1919..1930)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2084..2218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2121..2140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2175..2194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2524..3112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2604..2623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3283..4074,4075..4143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3906..3925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4144..4248)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4316..4334
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4372..4394)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4494..4513
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4580..5035)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4717..4738)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4929..4948
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gggggggggg gggggggggg ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       61 gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtggc caactccatc actaggggtt cctcagatcg ggctgcagga
      181 attggccgct ctagactcta gctgcagagg gacctgcgta tgagtgcaag tgggttttag
      241 gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa
      301 gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag
      361 gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc
      421 gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg
      481 tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc
      541 cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg
      601 cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct
      661 gagagcgcag tcgaattcaa gctgctagca aggatccacc ggtaccatgg cgaccctgga
      721 aaagctgatg aaggccttcg agtccctcaa gtccttccag cagcagcagc agcagcagca
      781 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
      841 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
      901 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc aacagccgcc
      961 accgccgccg ccgccgccgc cgcctcctca gcttcctcag ccgccgccgc aggcacagcc
     1021 gctgctgcct cagccgcagc cgcccccgcc gccgcccccg ccgccacccg gcccggctgt
     1081 ggctgaggag ccgctgcacc gaccaaagaa agaactttca gctaccaaga aagaccgtgt
     1141 gaatcattgt ctgacaatat gtgaaaacat agtggcacag tctgtcagaa attctccaga
     1201 atttcagaaa cttctgggca tcgctatgga actttttctg ctgtgcagtg atgacgcaga
     1261 gtcagatgtc aggatggtgg ctgacgaatg cctcaacaaa gttatcaaag ctttgatgga
     1321 ttctaatctt ccaaggttac agctcgagtc tagagggccc ttcgaacaaa aactcatctc
     1381 agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgaa ctagtggatc
     1441 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1501 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1561 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1621 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1681 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1741 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1801 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     1861 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     1921 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     1981 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     2041 aattcgagct cggtacagct tatcgatacc gtcgacttcg agcaacttgt ttattgcagc
     2101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgt
     2221 ctagcatcga agatcccccg atctgaggaa cccctagtga tggagttggc cactccctct
     2281 ctgcagcctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2701 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2941 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4141 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4261 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4321 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4381 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4441 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4501 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4561 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     4621 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     4681 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     4741 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4801 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4861 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4921 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     4981 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt
     5041 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5101 gccaggctgc a
//
