LOCUS       sequence-26139-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: Fret-Stop-Fret TOPO
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(74..107)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    169..290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(180..199)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     234..253
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgccttttgg tgncttatag aatactcaag ctatgcatca agcttggtac cgagctcgga
       61 tccgtcgacg tttgaagttc ctatactttc tagagaatag gaacttcgtt tctcgagctg
      121 cagacttact caagccatgg tggctagctt gcggaattcc agacatgata agatacattg
      181 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      241 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      301 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
      361 aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa
      421 atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata
      481 gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt
      541 ataactgtta tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag
      601 cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa
      661 cacancctgg ctcgaantca aanaacttna ccaattctga aggaagtcct tggcgccttc
      721 tacctgtctc tcctttttgg aggag
//
