LOCUS       sequence-261423-       11875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Tang X, Li J, Millan-Aguinaga N, Zhang JJ, O'Neill EC, Ugalde JA, 
            Jensen PR, Mantovani SM, Moore BS
  TITLE     Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways
            by Target-directed Genome Mining.
  JOURNAL   ACS Chem Biol. 2015 Oct 21.
  PUBMED    26458099
REFERENCE   2  (bases 1 to 11875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2015 Oct 21."
COMMENT     Sequence Label: pCAP03-acc(3)IV
FEATURES             Location/Qualifiers
     source          1..11875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    733..766
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     oriT            773..882
                     /note="incP origin of transfer"
     CDS             1198..1998
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSLAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     protein_bind    2008..2054
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2077..2880
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    2958..3461
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     3502..3520
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3558..3580)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3680..3699
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3720..4000
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             4001..4675
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(4915..4936)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5183..5771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5672..5691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5925..5942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6156..6290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6180..6199)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6234..6253
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6715..6735)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6865..6930
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(7350..8144)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7461..7480)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8071..8090
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(8928..9299)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9332..9441
                     /note="incP origin of transfer"
     CDS             complement(9679..9690)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    9761..9860
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             9870..11687
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 cctacaacaa ctaagaaaat ggctatcatg cggaagctgg tgagaagaac agcatcggga
       61 caagggaagg aagaacaaag acaaagaaaa caaaagaaag caattgaaaa caaaacaaaa
      121 caattttcat tccttctctt atcattcctt ttcttttctt ttctctcatt caacgcactc
      181 catcgtatcc gtattcctct tattttttct ctttctctat atccatttct ttctctctag
      241 gtgtgtcctc tctctctctt caatttctct actccgcatt ccaacgcatc cttcccccaa
      301 cctcccattt cctccttacg gcccgatagc gatcgtcttt ccctcgctat cactcgctac
      361 cggcccctcc tctgcaccgt aacctcctac gtatttacca tatcataaag ttttttccga
      421 cgcttatcgc tgaccccctg ccgccctcct attggcttcc ggattatctt cttgtccata
      481 aggtgatcca tgcttcctga agattcccga aatgtgtcca ctttggcggg gaatcattcc
      541 atccacttct ttctctctcg ctttcctcat tcggcgctcc ccttccgcgt ctcattggtc
      601 ttccgctccg tttttgcttt gccgatgtta cttggggaga ggtgcgataa tcctttcgca
      661 aaaactcggt ttgacgcctc ccatggtata aatagtggct cgagattccg gggatccgtc
      721 gacctgcagt tcgaagttcc tattctctag aaagtatagg aacttcgaag ttcccgccag
      781 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
      841 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
      901 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
      961 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaatgca
     1021 gctcacggta actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac
     1081 gctgaaaatg ccggcctttg aatgggttca tgtgcagctc cataagcaaa aggggatgat
     1141 aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg
     1201 tcattagcgg tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt
     1261 cagcttctca accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt
     1321 agcgtccggc ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctgcgctg
     1381 ggtccgggag ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat
     1441 cctgccacgt cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg
     1501 ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag
     1561 cagatcatct ctgatccatt gcccctgcca cctcactcgc ctgcaagccc ggtcgcccgt
     1621 gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg
     1681 ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt
     1741 cttcaggatg gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc
     1801 tttgccttgg cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt
     1861 catgcctttg ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg
     1921 ggccgagatc cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc
     1981 gctcgccagt cgattggctg agctcataag ttcctattcc gaagttccta ttctctagaa
     2041 agtataggaa cttcgaagca gctccagcct acacatatgt cgaaagctac atataaggaa
     2101 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
     2161 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
     2221 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
     2281 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
     2341 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
     2401 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
     2461 attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg
     2521 atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact
     2581 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
     2641 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
     2701 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
     2761 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
     2821 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
     2881 ggtacctgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2941 ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat
     3001 aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt
     3061 ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc
     3121 ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca
     3181 cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct
     3241 gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc
     3301 tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa
     3361 atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca
     3421 ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtctaaga aaccattatt
     3481 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     3541 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     3601 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     3661 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagatca
     3721 cgacattact atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg
     3781 tactttgcag ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc
     3841 ttgtcacctt acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa
     3901 ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac
     3961 gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac
     4021 aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg
     4081 tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac
     4141 aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc
     4201 aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc
     4261 tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga
     4321 ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa
     4381 catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga
     4441 agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca
     4501 agagagcccc gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg
     4561 tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa
     4621 tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat
     4681 tactgagtag tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat
     4741 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg
     4801 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     4861 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     4921 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     4981 tatcagggcg atggcccact acgtgaacct aactgtcaga ccaagtttac tcatatatac
     5041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5941 aggaagcgga agagcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     6001 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     6061 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     6121 ctcaacgaca ggagcacgat catgcgcacc cgtcagatcc agacatgata agatacattg
     6181 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6241 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     6301 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     6361 aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa
     6421 atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata
     6481 gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt
     6541 ataactgtta tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag
     6601 cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa
     6661 cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt
     6721 tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga
     6781 tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc actgctccca
     6841 ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca gtagtttaac
     6901 acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg taggtagttt
     6961 gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatccgga ccaaagcggc
     7021 catcgtgcct ccccactcct gcagttcggg ggcatggatg cgcggatagc cgctgctggt
     7081 ttcctggatg ccgacggatt tgcactgccg gtagaactcc gcgaggtcgt ccagcctcag
     7141 gcagcagctg aaccaactcg cgaggggatc gagcccgggg tgggcgaaga actccagcat
     7201 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     7261 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     7321 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     7381 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     7441 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     7501 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     7561 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catccgcgcc
     7621 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     7681 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     7741 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     7801 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     7861 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     7921 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca
     7981 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     8041 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     8101 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     8161 cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     8221 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     8281 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     8341 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     8401 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag
     8461 cagcccttgc gccctgagtg cttgcggcag cgtgagagct catgagcgga gaacgagatg
     8521 acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgtggagcgg atcggggatt
     8581 gtctttcttc agctcgctga tgatatgctg acgctcaatg ccgtttggcc tccgactaac
     8641 gaaaatcccg catttggacg gctgatccga ttggcacggc ggacggcgaa tggcggagca
     8701 gacgctcgtc cgggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtatcgggc
     8761 cctggccagc tagctagagt cgacctgcag gtccccgggg atcggtcttg ccttgctcgt
     8821 cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc atggggacgt
     8881 gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca tggctctgcc
     8941 ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc ttcgatcttc
     9001 gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc
     9061 cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc cagctcgcgg
     9121 acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc cagcaggtag
     9181 gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga
     9241 aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc
     9301 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
     9361 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     9421 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
     9481 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
     9541 ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgacctgc aggcatgcaa
     9601 gctctagcga ttccagacgt cccgaaggcg tggcgcggct tccccgtgcc ggagcaatcg
     9661 ccctgggtgg gttacacgac gcccctctat ggcccgtact gacggacaca ccgaagcccc
     9721 ggcggcaacc ctcagcggat gccccggggc ttcacgtttt cccaggtcag aagcggtttt
     9781 cgggagtagt gccccaactg gggtaacctt tgagttctct cagttggggg cgtagggtcg
     9841 ccgacatgac acaaggggtt gtgaccgggg tggacacgta cgcgggtgct tacgaccgtc
     9901 agtcgcgcga gcgcgaaaat tcgagcgcag caagcccagc gacacagcgt agcgccaacg
     9961 aagacaaggc ggccgacctt cagcgcgaag tcgagcgcga cgggggccgg ttcaggttcg
    10021 tcgggcattt cagcgaagcg ccgggcacgt cggcgttcgg gacggcggag cgcccggagt
    10081 tcgaacgcat cctgaacgaa tgccgcgccg ggcggctcaa catgatcatt gtctatgacg
    10141 tgtcgcgctt ctcgcgcctg aaggtcatgg acgcgattcc gattgtctcg gaattgctcg
    10201 ccctgggcgt gacgattgtt tccactcagg aaggcgtctt ccggcaggga aacgtcatgg
    10261 acctgattca cctgattatg cggctcgacg cgtcgcacaa agaatcttcg ctgaagtcgg
    10321 cgaagattct cgacacgaag aaccttcagc gcgaattggg cgggtacgtc ggcgggaagg
    10381 cgccttacgg cttcgagctt gtttcggaga cgaaggagat cacgcgcaac ggccgaatgg
    10441 tcaatgtcgt catcaacaag cttgcgcact cgaccactcc ccttaccgga cccttcgagt
    10501 tcgagcccga cgtaatccgg tggtggtggc gtgagatcaa gacgcacaaa caccttccct
    10561 tcaagccggg cagtcaagcc gccattcacc cgggcagcat cacggggctt tgtaagcgca
    10621 tggacgctga cgccgtgccg acccggggcg agacgattgg gaagaagacc gcttcaagcg
    10681 cctgggaccc ggcaaccgtt atgcgaatcc ttcgggcccc gcgtattgcg ggcttcgccg
    10741 ctgaggtgat ctacaagaag aagccggacg gcacgccgac cacgaagatt gagggttacc
    10801 gcattcagcg cgacccgatc acgctccggc cggtcgagct tgattgcgga ccgatcatcg
    10861 agcccgctga gtggtatgag cttcaggcgt ggttggacgg cagggggcgc ggcaaggggc
    10921 tttcccgggg gcaagccatt ctgtccgcca tggacaagct gtactgcgag tgtggcgccg
    10981 tcatgacttc gaagcgcggg gaagaatcga tcaaggactc ttaccgctgc cgtcgccgga
    11041 aggtggtcga cccgtccgca cctgggcagc acgaaggcac gtgcaacgtc agcatggcgg
    11101 cactcgacaa gttcgttgcg gaacgcatct tcaacaagat caggcacgcc gaaggcgacg
    11161 aagagacgtt ggcgcttctg tgggaagccg cccgacgctt cggcaagctc actgaggcgc
    11221 ctgagaagag cggcgaacgg gcgaaccttg ttgcggagcg cgccgacgcc ctgaacgccc
    11281 ttgaagagct gtacgaagac cgcgcggcag gcgcgtacga cggacccgtt ggcaggaagc
    11341 acttccggaa gcaacaggca gcgctgacgc tccggcagca aggggcggaa gagcggcttg
    11401 ccgaacttga agccgccgaa gccccgaagc ttccccttga ccaatggttc cccgaagacg
    11461 ccgacgctga cccgaccggc cctaagtcgt ggtgggggcg cgcgtcagta gacgacaagc
    11521 gcgtgttcgt cgggctcttc gtagacaaga tcgttgtcac gaagtcgact acgggcaggg
    11581 ggcagggaac gcccatcgag aagcgcgctt cgatcacgtg ggcgaagccg ccgaccgacg
    11641 acgacgaaga cgacgcccag gacggcacgg aagacgtagc ggcgtagcga gacacccggg
    11701 aagcctgatc tacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
    11761 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
    11821 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcga ctagt
//
