LOCUS       sequence-261429-        3976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - RoLuc Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pRoLuc
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1778..1796)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1779..1796)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1812..1828)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1812..1828)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(1943..2531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2023..2042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2646..2748
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2749..3408
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2815..2834)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3521..3949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3608..3627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3818..3839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaagctc acaacttgga
      121 tttcttctgc gcgagaagtt tgcgcaattc ggatcgaacc aacttgcctg tcgcgccttt
      181 aggtatatcg tctacgaaaa ctactccccc tcggatgcgt tttgtggggc taactctttc
      241 cgcaatgtaa tctatgatct cttgttcggt caatgatttt ccttcttgca acacaataca
      301 tgcccccggc aactcgccgg ctgcctcatc gggcacccca gttactcctg cgtccttaat
      361 gctgggatgt tggaggagga gccactcaag ctcggctggg gcgacttgat aacccttgta
      421 cttgatcagc tccttaagcc gatcaacgac aaaaaagtgg ccatcatcgt cgtagtaccc
      481 gacatcccca gagtggagcc acccttcctt atcaatggct tcttcggtgg cttgtttatt
      541 attaaagtag cccttcataa taatttcact tttgaagcac agttcacctc tcttgttagg
      601 ccccagagat tttcccgtgg taagatctac tatctttgct tgtacgtatg gcgcaacccg
      661 gcctgttgag ccggtcttga cgtcgtcgtg cggcgtgatg agcaccgcac aacacgtttc
      721 cgtgagtcca taaccttgca gaatgccgcc gattttcagc cgtttcgcta cggcaacggc
      781 tacttcggtc ccgactgggg caccgccggt ggcaacctcg cgtatagagg acaagtcata
      841 ctggtctacc aacggggatt tggcgaggaa taccataact ggtggcgcaa gcacgatggt
      901 cggaatgcgg tatttctcga tagtagacag gaaaaatttc tcttcgaagc gcttaaggag
      961 tactaccctc aagcccacga tgaagtaaga caatgttgtg aacattccga atgcatgatg
     1021 aaatggcact atactcagaa tggaagtact aggtattgtc cttgtcccga aaagtggatc
     1081 cttgcagtga acaaatctta ctgagaggtt tctgtgcgtg agtaccactc cttttggaag
     1141 tccggtggtc ccggaagagg acataatgag agcaacttgt tcctttgcat caaagtcgcg
     1201 gggtttaaaa ttccgaacgt ccaaatttgg ttcggaatac cgagccataa agttgctcag
     1261 acattgggcc tcgcccatat cctccttgga atcgaggagc acgactttct ttataaagtt
     1321 tacatttctt ttgagagcca tcatgttctt gattgctttt ttactgcaaa ataccaactc
     1381 cggctttgaa atgttgagcg tttcaagcaa ttcgcgttcg gtataagagt cattcacagt
     1441 ggcagtaatt acacccatat agagggcagc aatcacgggg tagaaaaaaa ttgttgagtt
     1501 ttcggagcaa acacttatca ctgagttttg gcgcaggccg taattctcat agcatttggc
     1561 aagtcggcag ctgttttcca atatatccgc ataactaaca acttcttcgg tgtgcgcatc
     1621 aatcaaggcc tcccgcaaaa aggagaagtt agtcagagcg cgatacaact ggatacccgc
     1681 cgtcccgagg tcgaggggat ctcggggctt tgccccgtgg agtatgattt cgttaggcat
     1741 tcgagaccac tcatcgccca atcactagtg aattcgttct atagtgtcac ctaaatacca
     1801 tagagccgtt ggtcatagct gtttcctgtc caggcatcat gacacggtta tccacagaat
     1861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2641 tgtgatgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     2701 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     2761 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     2821 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2881 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2941 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     3001 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     3061 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     3121 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     3181 gtgtcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     3241 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     3301 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     3361 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     3421 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg
     3481 aatggcagaa attcgatgat aagctgtcaa acatgatgtt acgcgccctg tagcggcgca
     3541 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3601 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3661 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3721 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt
     3781 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3841 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3901 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3961 ttaacgctta caattt
//
