LOCUS       sequence-261433-        2974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Lucia Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pLucia
FEATURES             Location/Qualifiers
     source          1..2974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(776..794)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(777..794)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(810..826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(810..826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(941..1529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1021..1040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1644..1746
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1747..2406
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1813..1832)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2519..2947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2606..2625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2816..2837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaagctc accgatctcc
      121 agccaatccc ttgatcttat cgactcgtcc ctggatcttg gatgcaaaag tcgtacagcg
      181 ttgcggcagc cactttttga gcaaatcgct gcattggaca ttagccagac ccttcagaca
      241 acccgtcgtg cagtcggcac agaggtccac ttgggcaata aactggtcca atggttcctt
      301 gtccttaaaa cctggtattt cagggatgtc aacgatagcc tctcctatgc caccctgtgc
      361 gctttccttt tcaccctcat acgtatgaca acgtcccgga ataaatttct tcattttagg
      421 tgtacatttg atatggctca ggcatatgag gcaaccgcga gtacaacctg cgcgacgcgc
      481 attcgcttcg agttcacgca ggacatcagg aggcaatttc tttccaggga gcttgccacg
      541 gtcagcgtcc gtattcactt gctcagtatc ggtggatata acgttcatag tctcccaatt
      601 cgtaaacaag tctgtctcga ggtccgttgt ggcgaagttg gaggctacgg ctgcaatgtt
      661 gaggtcctca tttatctctg tcggcttggc ctcggcaaca gcaatacaga tcagagcgaa
      721 cagaaccttg atctccattc gagaccactc atcgcccaat cactagtgaa ttcgttctat
      781 agtgtcacct aaataccata gagccgttgg tcatagctgt ttcctgtcca ggcatcatga
      841 cacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      901 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      961 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1021 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1081 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1141 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1201 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1261 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1321 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1381 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1441 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1501 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1561 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1621 tcttcaccta gatccttttg tgatgatcgg cacgtaagag gttccaactt tcaccataat
     1681 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     1741 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     1801 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     1861 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     1921 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     1981 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     2041 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     2101 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     2161 attgagaata tgtttttcgt gtcagccaat ccctgggtga gtttcaccag ttttgattta
     2221 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2281 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     2341 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     2401 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata
     2461 agtgataata agcggatgaa tggcagaaat tcgatgataa gctgtcaaac atgatgttac
     2521 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2581 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2641 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2701 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2761 gcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2821 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2881 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2941 gcgaatttta acaaaatatt aacgcttaca attt
//
