LOCUS       sequence-261439-        3791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of Lucia luciferase
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:Lucia:bgH
FEATURES             Location/Qualifiers
     source          1..3791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1257..1481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1601..2189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1681..1700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2304..2406
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2407..3222
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2407..3222
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2480..2499)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3336..3764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3423..3442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3633..3654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacaagctt cgtctcagtc aggagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatcaagctc aaatggagat caaggttctg ttcgctctga tctgtattgc
      661 tgttgccgag gccaagccga cagagataaa tgaggacctc aacattgcag ccgtagcctc
      721 caacttcgcc acaacggacc tcgagacaga cttgtttacg aattgggaga ctatgaacgt
      781 tatatccacc gatactgagc aagtgaatac ggacgctgac cgtggcaagc tccctggaaa
      841 gaaattgcct cctgatgtcc tgcgtgaact cgaagcgaat gcgcgtcgcg caggttgtac
      901 tcgcggttgc ctcatatgcc tgagccatat caaatgtaca cctaaaatga agaaatttat
      961 tccgggacgt tgtcatacgt atgagggtga aaaggaaagc gcacagggtg gcataggaga
     1021 ggctatcgtt gacatccctg aaataccagg ttttaaggac aaggaaccat tggaccagtt
     1081 tattgcccaa gtggacctct gtgccgactg cacgacgggt tgtctgaagg gtctggctaa
     1141 tgtccaatgc agcgatttgc tcaaaaagtg gctgccgcaa cgctgtacga cttttgcatc
     1201 caagatccag ggacgagtcg ataagatcaa gggattggct ggagatcggt gagcttctgt
     1261 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     1321 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     1381 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     1441 agacaatagc aggcatgctg gggatgcggt gggctctatg gcgcttgaga cgaagcttga
     1501 cacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2041 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2281 tcttcaccta gatccttttg tgatgatcgg cacgtaagag gttccaactt tcaccataat
     2341 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     2401 gctaaaatga gccatattca acgggaaacg tcttgctcaa ggccgcgatt aaattccaac
     2461 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     2521 acaatctacc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     2581 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     2641 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     2701 actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     2761 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     2821 tgtcctttta acagcgatcg cgtatttcga ctcgctcagg cgcaatcacg aatgaataac
     2881 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2941 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3001 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3061 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3121 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     3181 aataaattgc agtttcattt gatgctcgat gagtttttct gatttttttt aaggcagtta
     3241 ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg
     3301 cagaaattcg atgataagct gtcaaacatg atgttacgcg ccctgtagcg gcgcattaag
     3361 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3421 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3481 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3541 aaaacttgat tagggtgatg gttcacgtag tgggccagcg ccctgataga cggtttttcg
     3601 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3661 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3721 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3781 gcttacaatt t
//
