LOCUS       sequence-261442-        9565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Membrane-anchored TF used for yeast directed evolution of TEV 
            protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Sanchez MI, Ting AY
  TITLE     Directed evolution improves the catalytic efficiency of TEV 
            protease.
  JOURNAL   Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7.
  PUBMED    31819267
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7."
COMMENT     Sequence Label: 
            TDH3-STE2-Delta(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-
            tCYC
FEATURES             Location/Qualifiers
     source          1..9565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(354..1448)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFQNHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1449..1854)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     2050..2072
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2064..2081
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2065..2081
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2088..2107
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2088..2106
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2226..2892
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2762..2785
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             3871..3894
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(3904..3906,3907..3909,3910..4620)
                     /codon_start=1
                     /product="blue variant of GFP"
                     /label=BFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3949..3970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4210..4229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4557..4578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4645..5154
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             5788..5808
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             join(5818..6069,6070..6423)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     6361..6382
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             6610..6843
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      6887..7136
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6903..6921)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(7299..7317)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7338..7354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7338..7354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7351..7373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7362..7378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7637..7654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7808..8396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7888..7907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8575..9366,9367..9435))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9198..9217
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9436..9540)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc taactttttg tgtggtgccc tcctccttgt caatattaat
      121 gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt tgcttacctg
      181 tattccttta catcctcctt tttctccttc ttgataaatg tatgtagatt gcgtatatag
      241 tttcgtctac cctatgaaca tattccattt tgtaatttcg tgtcgcatat gtttctatta
      301 tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc tttttaagca
      361 aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact gttggaacca
      421 cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc ttcaatggcc
      481 ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga caagatagtg
      541 gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca tggttcgtac
      601 aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg caacaaaccc
      661 aaggaacctg ggataacgga ggcttcatcg gagatgatgt caccaaacat gttgctggtg
      721 attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc agaatcaatc
      781 aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc cacagttttt
      841 ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat aggcaatggt
      901 ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac ttctggaacg
      961 gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct cttaccaaag
     1021 taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc aaattgtggc
     1081 ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct taagttggcg
     1141 tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac actaccagta
     1201 ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt ggaggcttcc
     1261 agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc aattaaatga
     1321 ttttggaaat cgaacttgac attggaacga acatcagaaa tagctttaag aaccttaatg
     1381 gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat cttcttaggg
     1441 gcagacatta gaatggtata tccttgaaat atatatatat attgctgaaa tgtaaaaggt
     1501 aagaaaagtt agaaagtaag acgattgcta accacctatt ggaaaaaaca ataggtcctt
     1561 aaataatatt gtcaacttca agtattgtga tgcaagcatt tagtcatgaa cgcttctcta
     1621 ttctatatga aaagccggtt ccggcgctct cacctttcct ttttctccca atttttcagt
     1681 tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa
     1741 tttgattctg tgcgatagcg cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg
     1801 ttgctaagag attcgaactc ttgcatctta cgatacctga gtattcccac agttaactgc
     1861 ggtcaagata tttcttgaat caggcgcctt agaccgctcg gccaaacaac caattacttg
     1921 ttgagaaata gagtataatt atcctataaa tataacgttt ttgaacacac atgaacaagg
     1981 aagtacagga caattgattt tcgatcggat gtgctgcaag gcgattaagt tgggtaacgc
     2041 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
     2101 tatagggcga attggagaaa cagaataata tataaacctg tataatataa ccttgaagac
     2161 tatatttctt ttcgcggccg cctatagggc gaattggagc tcccgctcta gacagttcga
     2221 gtttatcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata gtagtgattt
     2281 tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc gtacatgccc
     2341 aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt gaacagttta
     2401 ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc agaaaaaaaa
     2461 agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc
     2521 ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg
     2581 agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac gcatgtatct
     2641 atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa
     2701 aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa gtatataaag
     2761 acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt
     2821 tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa taaacacaca
     2881 taaacaaaca aaactagtgg aacccccggg ctgcaggaat tcgatatcaa gcttatgtct
     2941 gatgcggctc cttcattgag caatctattt tatgatccaa cgtataatcc tggtcaaagc
     3001 accattaact acacttccat atatgggaat ggatctacca tcactttcga tgagttgcaa
     3061 ggtttagtta acagtactgt tactcaggcc attatgtttg gtgtcagatg tggtgcagct
     3121 gctttgactt tgattgtcat gtggatgaca tcgagaagca gaaaaacgcc gattttcatt
     3181 atcaaccaag tttcattgtt tttaatcatt ttgcattctg cactctattt taaatattta
     3241 ctgtctaatt actcttcagt gacttacgct ctcaccggat ttcctcagtt catcagtaga
     3301 ggtgacgttc atgtttatgg tgctacaaat ataattcaag tccttcttgt ggcttctatt
     3361 gagacttcac tggtgtttca gataaaagtt attttcacag gcgacaactt caaaaggata
     3421 ggtttgatgc tgacgtcgat atctttcact ttagggattg ctacagttac catgtatttt
     3481 gtaagcgctg ttaaaggtat gattgtgact tataatgatg ttagtgccac ccaagataaa
     3541 tacttcaatg catccacaat tttacttgca tcctcaataa actttatgtc atttgtcctg
     3601 gtagttaaat tgattttagc tattagatca agaagattcc ttggtctcaa gcagttcgat
     3661 agtttccata ttttactcat aatgtcatgt caatctttgt tggttccatc gataatattc
     3721 atcctcgcat acagtttgaa accaaaccag ggaacagatg tcttgactac tgttgcaaca
     3781 ttacttgctg tattgtcttt accattatca tcaatgtggg ccacggctgc taatggaggg
     3841 tcaggcacag gtggctctcc atggggttca gattacaagg atgacgacga taaggggtct
     3901 ggaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3961 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     4021 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     4081 accctcgtga ccaccctgag ccacggcgtc cagtgcttca gccgctaccc cgaccacatg
     4141 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     4201 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     4261 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     4321 cacaagctgg agtacaactt caacagccac aacgtctata tcatggccga caagcagaag
     4381 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     4441 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacagc
     4501 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     4561 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4621 ccatggggat ctggcacagg cgggatgaat ggagctatag gaggtgacct tttgctcaat
     4681 tttcctgaca tgtcggtcct agagcgccaa agggctcacc tcaagtacct caatcccacc
     4741 tttgattctc ctctcgccgg cttctttgcc gattcttcaa tgattaccgg cggcgagatg
     4801 gacagctatc tttcgactgc cggtttgaat cttccgatga tgtacggtga gacgacggtg
     4861 gaaggtgatt caagactctc aatttcgccg gaaacgacgc ttgggactgg aaatttcaag
     4921 gcagcgaagt ttgatacaga gactaaggat tgtaatgagg cggcgaagaa gatgacgatg
     4981 aacagagatg acctagtaga agaaggagaa gaagagaagt cgaaaataac agagcaaaac
     5041 aatgggagca caaaaagcat caagaagatg aaacacaaag ccaagaaaga agagaacaat
     5101 ttctctaatg attcatctaa agtgacgaag gaattggaga aaacggatta tattcatggt
     5161 ggctctggta gcttcaacgc tcgcaggaag ctggctggtg ctatcctgac taccatgctc
     5221 ttcacaagaa acttcagcgg cagctttaat gcgcggcgca agctagccgg agccatactt
     5281 acgacaatgt tgtttaccag aaatttttcg gagctggccg agaagctggc cggcctggac
     5341 atcaacggtg gagctagcgg cagccgggcg accaccctgg aacgtattga aaagagcttt
     5401 gtgattaccg atccgcgtct gccggataac ccgattattt ttgtgagcga tagctttctg
     5461 cagctgaccg aatatagccg tgaagaaatt ctgggccgta actgccgttt tctgcagggc
     5521 ccggaaaccg atcgtgcgac cgtgcgtaaa attcgtgatg cgattgataa ccagaccgaa
     5581 gtgaccgtgc agctgattaa ctataccaaa agcggcaaaa aattttggaa cctgtttcat
     5641 ctgcagccga tgcgtgatca gaaaggcgat gtgcagtatt ttattggcgt gcagctggat
     5701 ggtaccgaac gtgtgcgtga tgcggcggaa cgtgaagctg tgatgctggt caagaaaacc
     5761 gcggaggaaa tcgacgaagc cgctaaggaa aatctctact tccagatggg cgggcctatg
     5821 aaagcgttaa cggccaggca acaagaggtg tttgatctca tccgtgatca catcagccag
     5881 acaggtatgc cgccgacgcg tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac
     5941 gcggctgaag aacatctgaa ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc
     6001 gcatcacgcg ggattcgtct gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt
     6061 gtggctgccg gtgaaccact tctggcgcaa cagcatattg aaggtcatta tcaggtcgat
     6121 ccttccttat tcaagccgaa tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa
     6181 gacatcggca ttatggatgg tgacttgctg gcagtgcata aaactcagga tgtacgtaac
     6241 ggtcaggtcg ttgtcgcacg tattgatgac gaggttaccg ttaagcgcct gaaaaaacag
     6301 ggcaataaag tcgaactgtt gccagaaaat agcgagttta aaccaattgt cgtagatctt
     6361 cgtcagcaga gcttcaccat tgaagggctg gcggttgggg ttattcgcaa cggcgactgg
     6421 ctgggtgata tcatgcagga tctgccgggc aacgataaca gcaccgcggg cgcgcgtacg
     6481 aaaaacaatt acgggtctac catcgagggc ctgctcgatc tcccggacga cgacgccccc
     6541 gaagaggcgg ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca cacgcgcaga
     6601 ctgtcgacgg cccccccgac cgatgtcagc ctgggggacg aattgcactt agacggcgag
     6661 gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac
     6721 ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat
     6781 atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga cgagtacggt
     6841 gggaagctct aatagctcga gacaggcccc ttttcctttg tctatatcat gtaattagtt
     6901 atgtcacgct tacattcacg ccctcctccc acatccgctc taaccgaaaa ggaaggagtt
     6961 agacaacctg aagtctaggt ccctatttat tttttttaat agttatgtta gtattaagaa
     7021 cgttatttat atttcaaatt tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca
     7081 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagg
     7141 ttcgcagttt acactctcat cgtcgctctc atcatcgctt ccgttgttgt tttccttagt
     7201 agcgtctgct tggtacccag cttcggccgt atttacagat aacgatgtcg ttattatata
     7261 tatatatata tattgtcact tgtacccagc ttttgttccc tttagtgagg gttaatttcg
     7321 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaaca
     7381 gctgtaatat atagtagtaa cctgaaaaat agtcaatgcc cgtggttcat ttgccaatag
     7441 aacagtcgat gtgatcaatg tacacgaaag tcaatataaa atatccaaat agccaatggt
     7501 gtcaggacgg ctaaggcaaa tgcaactttg aaatacttat tcggtttgca tcttggcgcg
     7561 cctcttcctg caggcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7621 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7741 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7801 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7861 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7921 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7981 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8041 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8101 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8161 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8221 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8401 atcctttgat cttttgttta aacctacggg gtctgacgct cagtggaacg aaaactcacg
     8461 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8521 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8581 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8641 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8701 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8761 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8821 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8881 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8941 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9001 ctccttcggt cctccgatgg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9061 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9121 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9181 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9241 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9301 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9361 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9421 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9481 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9541 cacatttccc cgaaaagtgc cacct
//
