LOCUS       sequence-26145-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) 
            fused to EYFP, for bi-stable optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Berndt A, Yizhar O, Gunaydin LA, Hegemann P, Deisseroth K
  TITLE     Bi-stable neural state switches.
  JOURNAL   Nat Neurosci. 2009 Feb . 12(2):229-34.
  PUBMED    19079251
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2009 Feb . 12(2):229-34."
COMMENT     Sequence Label: pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccgggtangc tctcgccctg ctcaccatgg tggcggccgc tggcacggct ccggcctcgg
       61 cttcgtcttc gacgagagtc tcgacctcga tctccgttcc gccgatgttc agtttggtgg
      121 ttttgcggat atctccgtga atcaatatgt gctcgtggat caggacgcgc aggtagtgtc
      181 ccaacaaccc ccaacaattt ttactcatca gatcaataat cgtgtgacct acggtggagc
      241 catagacgct caggacgcca aaaccttcgg gccccaaaat gaagagaatt gggaacatac
      301 cccagctcac gaaaaacagc catgccatgc cggtcacgac ctggcggcac cgaccctttg
      361 gcacagtatg ataaccctcg atatatgctt tggcggcgtg aaaaaatgtg ttcgcgccat
      421 agcacaatcc aagacaaaag aagatgactt taacatagcc ggttgccatg gcgctggtag
      481 ccccccacac gatagtcccg atgtctgaga caaggagtcc catggttctc ctgctgtagt
      541 cgttgctcag gccggtgagg ttgctcaggt ggataaggat gacaggagta gtgagcagcc
      601 actctgcata gcgcagccac tgcacccggt gtcctgtggc aaggtagagc atagagggat
      661 tcttaaactc aaaaaagaac tcgagaatca ccttaaccat ttcaatggcg cacacataga
      721 tctcctccca gccgcatgta gatttccagg tttggtaggc atagaacatc agcagcaaat
      781 gctgatcctg ctgcagcact gcagaattga ncggtctgac gcgtctgcgc gagatcatca
      841 tcggaagtac atgactagga cagacgaccg tccaccagat atacacaag
//
