LOCUS       sequence-261450-        9690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Interacts with cytoplasmic DNA. Useful for identifying sites of 
            nuclear envelope rupture..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Denais CM, Gilbert RM, Isermann P, McGregor AL, te Lindert M, 
            Weigelin B, Davidson PM, Friedl P, Wolf K, Lammerding J
  TITLE     Nuclear envelope rupture and repair during cancer cell migration.
  JOURNAL   Science. 2016 Apr 15;352(6283):353-8. doi: 10.1126/science.aad7297. 
            Epub 2016 Mar 24.
  PUBMED    27013428
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad7297"; journalName: "Science"; date: 
            "2016-04-15- 15"; volume: "352"; issue: "6283"; pages: "353-8"
COMMENT     Sequence Label: pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..1984
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1935..1955
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1981..2005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3654..3663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3660..4370
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3805..3823)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        4412..4623
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4636..4904
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4931..5530
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4931..4950)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5427..5447
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5537..6125
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5590..5610)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6008..6019)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6199..6432
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6504..6625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6541..6560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6595..6614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6665..6800
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6833..6849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6833..6849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6846..6868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6857..6873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6881..6887,6888..6905,6906..6911))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6926..6947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7064..7081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7235..7823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7994..8785,8786..8854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8617..8636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8855..8959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9027..9045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9083..9105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9205..9224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9418..9440
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9432..9449
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9433..9449
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9464..9690
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgatact agtattatgc ccagtacatg accttatggg actttcctac
     1741 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1801 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1861 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1921 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag
     1981 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2041 tagaagattc tagaaccatg cagccttggc acggaaaggc catgcagaga gcttccgagg
     2101 ccggagccac tgcccccaag gcttccgcac ggaatgccag gggcgccccg atggatccca
     2161 acgagtctcc ggctgccccc gaggccgccc tgcctaaggc gggaaagttc ggccccgcca
     2221 ggaagtcggg atcccggcag aaaaagagcg ccccggacac ccaggagagg ccgcccgtcc
     2281 gcgcaactgg ggcccgcgcc aaaaaggccc ctcagcgcgc ccaggacacg cagccgtctg
     2341 acgccaccag cgcccctggg gcagaggggc tggagcctcc tgcggctcgg gagccggctc
     2401 tttccagggc tggttcttgc cgccagaggg gcgcgcgctg ctccacgaag ccaagacccc
     2461 cgcccgggcc ctgggacgtg cccagccccg gcctgccggt ctcggccccc attctcgtac
     2521 ggagggatgc ggcgcctggg gcctcgaagc tccgggcggt tttggagaag ttgaagctca
     2581 gccgcgatga tatctccacg gcggcgggga tggtgaaagg ggttgtggac cacctgctgc
     2641 tcagactgaa gtgcgactcc gcgttcagag gcgtcgggct gctgaacacc gggagctact
     2701 atgagcacgt gaagatttct gcacctaatg catttgctgt catgtttaaa ctggaagtcc
     2761 ccagaattca actagaagaa tattccaaca ctcgtgcata ttactttgtg aaatttaaaa
     2821 gaaatccgaa agaaaatcat ctgagtcagt ttttagaagg tgaaatatta tcagcttcta
     2881 agatgctgtc aaagtttagg aaaatcatta aggaagaaat taacgacatt aaagatacag
     2941 atgtcatcat gaagaggaaa agaggaggga gccctgctgt aacacttctt attagtgaaa
     3001 aaatatctgt ggatataacc ctggctttgg aatcaaaaag tagctggcct gctagcaccc
     3061 aagaaggcct gcgcattcaa aactggcttt cagcaaaagt taggaagcaa ctacgactaa
     3121 agccatttta ccttgtaccc aagcatgcaa aggaaggaaa tggtttccaa gaagaaacat
     3181 ggcggctatc cttctctcac atcgaaaagg aaattttgaa caatcatgga aaatctaaaa
     3241 cgtgctgtga aaacaaagaa gagaaatgtt gcaggaaaga ttgtttaaaa ctaatgaaat
     3301 accttttaga acagctgaaa gaaaggttta aagacaaaaa acatctggat aaattctctt
     3361 cttatcatgt gaaaactgcc ttctttcacg tatgtaccca gaaccctcaa gacagtcagt
     3421 gggaccgcaa agacctgggc ctctgctttg ataactgcgt gacatacttt cttcagtgcc
     3481 tcaggacaga aaaacttgag aattatttta ttcctgaatt caatctattc tctagcaact
     3541 taattgacaa aagaagtaaa gaatttctga caaagcaaat tgaatatgaa agaaacaatg
     3601 agtttccagt ttttgatgaa tttacggtac cgcgggcccg ggatccaccg gtcgccacca
     3661 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     3721 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     3781 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     3841 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     3901 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaat tgggagcgcg
     3961 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     4021 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     4081 tgcagtgtcg taccatgggc tgggaggcct ccactgagcg gatgtacccc gaggacggcg
     4141 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     4201 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtcg
     4261 acatcaagtt ggacatcctt tcccacaacg aggactacac catcgtggaa cagtacgaac
     4321 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa gcggccgcga
     4381 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4441 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4501 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4561 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4621 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4681 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4741 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     4801 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     4861 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     4921 cctacgctag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4981 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5041 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5101 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5161 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5221 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5281 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5341 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5401 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5461 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5521 cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5581 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5641 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5701 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5761 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5821 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5881 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     5941 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6001 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6061 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6121 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6181 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt
     6241 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6301 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6361 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6421 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6481 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     6541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6601 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     6661 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6721 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6781 aggaggcttt tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc
     6841 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6901 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6961 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9001 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     9061 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9121 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9181 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9241 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     9301 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9361 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9421 agtcacgacg ttgtaaaacg acggccagtg ccaagctgac gcgtgtagtc ttatgcaata
     9481 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     9541 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     9601 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     9661 gtatttaagt gcctagctcg atacataaac
//
